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The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells

  • Authors:
    • Tein-Ming Yuan
    • Ruei-Yue Liang
    • Nai-Wan Hsiao
    • Show-Mei Chuang
  • View Affiliations / Copyright

    Affiliations: Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, R.O.C., Institute of Biotechnology, National Changhua University of Education, Changhua 50007, Taiwan, R.O.C.
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2307-2318
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    Published online on: October 13, 2014
       https://doi.org/10.3892/or.2014.3540
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Abstract

Upregulation of the metastasis-promoting S100A4 protein has been linked to tumor migration and invasion, and clinical studies have demonstrated that significant expression of S100A4 in primary tumors is indicative of poor prognosis. However, the involvement of S100A4 in the drug responsiveness of gastric cancer remains unclear. In the present study, we used gastric cancer cell lines as a model to investigate the involvement of S100A4 in drug responsiveness. We overexpressed S100A4 in AGS and SCM-1 cells, which are characterized by relatively low-level expression of endogenous S100A4, and found that this significantly enhanced cell migration but did not affect cell survival in the presence of six common anticancer drugs. Moreover, in vitro cell proliferation was unchanged. Using RNA interference, we suppressed S100A4 expression in MKN-45 and TMK-1 cells (which are characterized by high-level expression of endogenous S100A4), and found that knockdown of S100A4 markedly attenuated cell motility but did not affect cell survival in the presence of six common anticancer drugs. Further study revealed that a single nucleotide polymorphism (SNP) of S100A4 (rs1803245; c.29A>T), which substitutes an Asp residue with Val (D10V), is localized within the conserved binding surface for Annexin II. Cells overexpressing S100A4D10V showed a significant reduction in cell migration ability, but no change in cell survival, upon anticancer drug treatment. Taken together, our novel results indicate that the expression level of S100A4 does not significantly affect cell survival following anticancer drug treatment. Thus, depending on the cell context, the metastasis-promoting effects of S100A4 may not be positively correlated with anticancer drug resistance in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan T, Liang R, Hsiao N and Chuang S: The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncol Rep 32: 2307-2318, 2014.
APA
Yuan, T., Liang, R., Hsiao, N., & Chuang, S. (2014). The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncology Reports, 32, 2307-2318. https://doi.org/10.3892/or.2014.3540
MLA
Yuan, T., Liang, R., Hsiao, N., Chuang, S."The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells". Oncology Reports 32.6 (2014): 2307-2318.
Chicago
Yuan, T., Liang, R., Hsiao, N., Chuang, S."The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells". Oncology Reports 32, no. 6 (2014): 2307-2318. https://doi.org/10.3892/or.2014.3540
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan T, Liang R, Hsiao N and Chuang S: The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncol Rep 32: 2307-2318, 2014.
APA
Yuan, T., Liang, R., Hsiao, N., & Chuang, S. (2014). The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncology Reports, 32, 2307-2318. https://doi.org/10.3892/or.2014.3540
MLA
Yuan, T., Liang, R., Hsiao, N., Chuang, S."The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells". Oncology Reports 32.6 (2014): 2307-2318.
Chicago
Yuan, T., Liang, R., Hsiao, N., Chuang, S."The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells". Oncology Reports 32, no. 6 (2014): 2307-2318. https://doi.org/10.3892/or.2014.3540
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