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Article

Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p

  • Authors:
    • Yanming Pan
    • Fengyun Ren
    • Wei Zhang
    • Guibo Liu
    • Dan Yang
    • Jing Hu
    • Kejian Feng
    • Yukuan Feng
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Tumor Prevention and Treatment (Heilongjiang Higher Education Institutions), Mudanjiang Medical University, Mudanjiang 157011, P.R. China, Department of Respiration, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157011, P.R. China, School of Basic Medical Science, Mudanjiang Medical University, Mudanjiang 157011, P.R. China
  • Pages: 2549-2556
    |
    Published online on: October 14, 2014
       https://doi.org/10.3892/or.2014.3546
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Abstract

MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assessed via microarray and bioinformatics analysis, and western blotting. The protein content detection showed that miRNA‑135a-5p expression was inversely correlated with AP-2α. Bioinformatics analysis revealed that AP-2α contains a putative miRNA-135a-5p target, which was confirmed as a direct target using the 3'-UTR luciferase reporter system. Additionally, an increase and decrease of miRNA-135a-5p inhi­bited or impaired adriamycin-induced apoptosis in BGC-823 cells (p<0.05, compared with the group without gene intervention), respectively. Luciferase reporter experiments confirmed that AP-2α bound to the BCL-2 promoter and affected its transcription. Therefore, miRNA-135a-5p increased BCL-2 via AP-2α and consequently enhanced cell resistance to apoptosis. This newly identified miRNA-135a-5p-AP-2α-BCL-2 pathway provides insight for the treatment of gastric cancer and solution for insensitivity of gastric cancer to chemotherapy drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Pan Y, Ren F, Zhang W, Liu G, Yang D, Hu J, Feng K and Feng Y: Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p. Oncol Rep 32: 2549-2556, 2014.
APA
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J. ... Feng, Y. (2014). Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p. Oncology Reports, 32, 2549-2556. https://doi.org/10.3892/or.2014.3546
MLA
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J., Feng, K., Feng, Y."Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p". Oncology Reports 32.6 (2014): 2549-2556.
Chicago
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J., Feng, K., Feng, Y."Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p". Oncology Reports 32, no. 6 (2014): 2549-2556. https://doi.org/10.3892/or.2014.3546
Copy and paste a formatted citation
x
Spandidos Publications style
Pan Y, Ren F, Zhang W, Liu G, Yang D, Hu J, Feng K and Feng Y: Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p. Oncol Rep 32: 2549-2556, 2014.
APA
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J. ... Feng, Y. (2014). Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p. Oncology Reports, 32, 2549-2556. https://doi.org/10.3892/or.2014.3546
MLA
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J., Feng, K., Feng, Y."Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p". Oncology Reports 32.6 (2014): 2549-2556.
Chicago
Pan, Y., Ren, F., Zhang, W., Liu, G., Yang, D., Hu, J., Feng, K., Feng, Y."Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p". Oncology Reports 32, no. 6 (2014): 2549-2556. https://doi.org/10.3892/or.2014.3546
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