Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
January-2015 Volume 33 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 33 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma

  • Authors:
    • Peng-Xi Li
    • Jiang-Hong Mu
    • Hua-Lang Xiao
    • Dong-Hong Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Trauma, Burn and Combined Injury, The Second Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, Sichuan 400042, P.R. China, Department of Pathology, Daping Hospital, Third Military Medical University, Chongqing, Sichuan 400042, P.R. China
  • Pages: 125-132
    |
    Published online on: November 7, 2014
       https://doi.org/10.3892/or.2014.3593
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The antitumor effect of photodynamic therapy (PDT) mediated by a novel photosensitizer I (Ps I; {γ-[N-poly(ethyleneglycol)]folic acid}-5,10,15-tris(3-hydroxyphenyl)-20-(4-carboxyphenyl)chlorin), in which chlorin was used as a photoactive unit, folic acid as a tumor‑targeting warhead, and polyethylene glycol as a linker, on cervical carcinoma was studied in vitro and in vivo. Ps I exhibited a considerably higher cellular uptake by HeLa cells than folic acid-free analogue Ps A (tert-butyl N-poly(ethyleneglycol)ethylcarbamate-5,10,15-tris(3-hydroxyphenyl)-20-(4-carboxyphenyl)chlorin), and the cellular uptake by HeLa cells of Ps I could be competitively inhibited by excess folic acid. Moreover, at different time points after the intravenous (i.v.) injection of Ps I and A, Ps I produced a >2-fold higher tumor to normal tissue ratio in tumor-bearing nude mice as compared to Ps A. MTT assay indicated that the HeLa cell proliferation inhibition ratio was increased 34% after Ps I-PDT compared with Ps A-PDT with a photosensitizer concentration of 15.2 µmol/l. Administration of Ps I (7 mg/kg, i.v.) followed by light exposure (80 J/cm2) markedly suppressed the growth of xenograft tumors, and the tumor volume was 10-fold smaller than that of the control group. Tumor growth inhibition in vitro and in vivo had an obvious dependency on the Ps I concentration and irradiation dose. The mode of cell death post-Ps I-PDT was analyzed by flow cytometry, confocal laser scanning microscopy, and electron microscope, and the results suggested that apoptosis was the primary mode of HeLa cell death induced by Ps I-PDT. The results also demonstrated that tumor targeting of Ps I was clearly improved because of the endocytosis mediated by the folate receptor. As a result, Ps I-PDT exhibited higher antitumor activity than Ps A-PDT and has potential as an alternative treatment modality for cervical cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Downs LS, Smith JS, Scarinci I, Flowers L and Parham G: The disparity of cervical cancer in diverse populations. Gynecol Oncol. 109:22–30. 2008. View Article : Google Scholar

2 

Wells SF: Cervical cancer: an overview with suggested practice and policy goals. Medsurg Nurs. 17:43–50. 2008.PubMed/NCBI

3 

He GF, Bian ML, Zhao YW, Xiang Q, Li HY and Xiao C: A study on the mechanism of 5-aminolevulinic acid photodynamic therapy in vitro and in vivo in cervical cancer. Oncol Rep. 21:861–868. 2009.PubMed/NCBI

4 

Fayter D, Corbett M, Heirs M, Fox D and Eastwood A: A systematic review of photodynamic therapy in the treatment of pre-cancerous skin conditions, Barrett’s oesophagus and cancers of the biliary tract, brain, head and neck, lung, oesophagus and skin. Health Technol Asses. 14:1–288. 2010.

5 

Benov L: Photodynamic therapy: current status and future directions. Med Princ Pract. May 10–2014.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

6 

Syu WJ, Yu HP, Hsu CY, et al: Improved photodynamic cancer treatment by folate conjugated polymeric micelles in a KB xeno-grafted animal model. Small. 8:2060–2069. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Dabrowski JM, Arnaut LG, Pereira MM, Monteiro CJ, Urbanska K, Simoes S and Stochel G: New halogenated water-soluble chlorin and bacteriochlorin as photostable PDT sensitizers: synthesis, spectroscopy, photophysics, and in vitro photosensitizing efficacy. Chem Med Chem. 5:1770–1780. 2010. View Article : Google Scholar

8 

Eshghi H, Sazgarnia MSc A, Rahimizadeh M, Attaran N, Bakavoli M and Soudmand S: Protoporphydin IX-gold nanoparticle conjugates as an efficient photosensitizer in cervial cancer therapy. Photodiagnosis Photodyn Ther. 10:304–312. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Shupin-Mrugalska P, Piskorz J, Goslinski T, Mielcarek J, Konopka K and Düzgüneş N: Current status of liposomal porphyrinoid photosensitizers. Drug Discov Today. 18:776–784. 2013. View Article : Google Scholar

10 

Gijsens A, Missiaen L, Merlevede W and de Witte P: Epidermal growth factor-mediated targeting of chlorin e6 selectively potentiates its photodynamic activity. Cancer Res. 60:2197–2202. 2000.PubMed/NCBI

11 

Stefflova K, Li H, Chen J and Zheng G: Peptide-based pharmaco-modulation of a cancer-targeted optical imaging and photodynamic therapy agent. Bioconjug Chem. 18:379–388. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Vrouenraets MB, Visser GWM, Loup C, et al: Targeting of a hydrophilic photosensitizer by use of internalizing monoclonal antibodies: A new possibility for use in photodynamic therapy. Int J Cancer. 88:108–114. 2000. View Article : Google Scholar : PubMed/NCBI

13 

McCarthy JR, Bhaumik J, Merbouh N and Weissleder R: High-yielding syntheses of hydrophilic, conjugatable chlorins and bacteriochlorins. Org Biomol Chem. 7:3430–3436. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Vlahov IR and Leamon CP: Engineering folate-drug conjugates to target cancer: from chemistry to clinic. Bioconjug Chem. 23:1357–1369. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Bugaj AM: Targeted photodynamic therapy - a promising strategy of tumor treatment. Photochem Photobiol Sci. 10:1097–1109. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Leung WN, Sun X, Mak NK and Yow CM: Photodynamic effects of mTHPC on human colon adenocarcinoma cells: photo-cytotoxicity, subcellular localization and apoptosis. Photochem Photobiol. 75:406–411. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Wang L, Li M and Zhang N: Folate-targeted docetaxel-lipid-based-nanosuspensions for active-targeted cancer therapy. Int J Nanomedicine. 7:3281–3294. 2012.PubMed/NCBI

18 

Li D, LI P, Lin H, Jiang Z, Guo L and Li B: A novel chlorin-PEG-folate conjugate with higher water solubility, lower cytotoxicity, better tumor targeting and photodynamic activity. J Photochem Photobiol B. 127:28–37. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Vaidya A, Sun Y, Feng Y, Emerson L, Jeong EK and Lu ZR: Contrast-enhanced MRI-guided photodynamic cancer therapy with a pegylated bifunctional polymer conjugate. Pharm Res. 25:2002–2011. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Chin WWL, Heng PWS, Thong PSP, et al: Improved formulation of photosensitizer chlorin e6 polyvinylpyrrolidone for fluorescence diagnostic imaging and photodynamic therapy of human cancer. Eur J Pharm Biopharm. 69:1083–1093. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Gui T, Wang Y, Mao Y, Liu J, Sun S, Cao D, Yang J and Shen K: Comparisons of 5-aminolevulinic acid photodynamic therapy and after-loading radiotherapy in vivo in cervical cancer. Clin Transl Oncol. 15:434–442. 2013. View Article : Google Scholar

22 

Hiorth M, Liereng L, Reinertsen R and Tho I: Formulation of bioadhesive hexylaminolevulinate pellets intended for photo-dynamic therapy in treatment of cervical cancer. Int J Pharm. 441:544–554. 2013. View Article : Google Scholar

23 

Saul JM, Annapragada A, Natarajan JV and Bellamkonda RV: Controlled targeting of liposomal doxorubicin via the folate receptor in vitro. J Control Release. 92:49–67. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Westermann P, Glanzmann T, Andrejevic S, et al: Long circulating half-life and high tumor selectivity of the photosensitizer meta-tetrahydroxyphenylchlorin conjugated to polyethylene glycol in nude mice grafted with a human colon carcinoma. Int J Cancer. 76:842–850. 1998. View Article : Google Scholar

25 

Evensen JF, Moan J, Hindar A and Sommer S: Tissue distribution of 3H-hematoporphyrin derivative and its main components, 67Ga- and 131I-albumin in mice bearing Lewis lung carcinoma. Prog Clin Biol Res. 170:541–562. 1984.

26 

Leamon CP and Reddy JA: Folate-targeted chemotherapy. Adv Drug Deliv Rev. 56:1127–1141. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Zhang JL, Deng L, Yao JZ, et al: Synthesis and photobiological study of a novel chlorin photosensitizer BCPD-18MA for photo-dynamic therapy. Bioorg Med Chem. 19:5520–5528. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li P, Mu J, Xiao H and Li D: Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma. Oncol Rep 33: 125-132, 2015.
APA
Li, P., Mu, J., Xiao, H., & Li, D. (2015). Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma. Oncology Reports, 33, 125-132. https://doi.org/10.3892/or.2014.3593
MLA
Li, P., Mu, J., Xiao, H., Li, D."Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma". Oncology Reports 33.1 (2015): 125-132.
Chicago
Li, P., Mu, J., Xiao, H., Li, D."Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma". Oncology Reports 33, no. 1 (2015): 125-132. https://doi.org/10.3892/or.2014.3593
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Mu J, Xiao H and Li D: Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma. Oncol Rep 33: 125-132, 2015.
APA
Li, P., Mu, J., Xiao, H., & Li, D. (2015). Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma. Oncology Reports, 33, 125-132. https://doi.org/10.3892/or.2014.3593
MLA
Li, P., Mu, J., Xiao, H., Li, D."Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma". Oncology Reports 33.1 (2015): 125-132.
Chicago
Li, P., Mu, J., Xiao, H., Li, D."Antitumor effect of photodynamic therapy with a novel targeted photosensitizer on cervical carcinoma". Oncology Reports 33, no. 1 (2015): 125-132. https://doi.org/10.3892/or.2014.3593
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team