Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells

Liu,Xiaojun; Yang,Zhongxia; Chen,Zhaofeng; Chen,Rui; Zhao,Da; Zhou,Yongning; Qiao,Liang

doi:10.3892/or.2014.3600

Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells

Authors:
- Xiaojun Liu
- Zhongxia Yang
- Zhaofeng Chen
- Rui Chen
- Da Zhao
- Yongning Zhou
- Liang Qiao
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Affiliations: Department of Medical Oncology, The First Hospital of Lanzhou University, Lanzhou, Gansu, P.R. China, Department of Infection, The First Hospital of Lanzhou University, Lanzhou, Gansu, P.R. China, Key Laboratory for Gastrointestinal Disease of Gansu Province, Lanzhou, Gansu, P.R. China, Storr Liver Unit, Westmead Millennium Institute, Western Clinical School of the Faculty of Medicine at Westmead Hospital, The University of Sydney, Westmead, NSW, Australia
Published online on: November 12, 2014 https://doi.org/10.3892/or.2014.3600
Pages: 157-162

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Abstract

Lactate dehydrogenase A (LDH-A), which regulates glycolytic flux by catalyzing pyruvate to lactate in the cytoplasm, is believed to be one of the highly attractive therapeutic targets for cancers. Firstly, we detected the expression of LDH-A in gastric cancer (GC) cells. LDH-A inhibitor oxamate was then used to suppress the LDH-A activity in GC cells. Cell proliferation, lactic acid production, Transwell migration assay and apoptosis were assessed, respectively. The results showed that inhibition of LDH-A by oxamate decreased the lactate production. In the presence of glucose, oxamate inhibited cell proliferation in a dose-dependent manner. Flow cytometry assay further confirmed a pro-apoptotic effect of oxamate, and this was likely through increased expression of Bax, activated caspase-3, and decreased expression of Bcl-2. Therefore, we believe that oxamate inhibits cell growth, suppresses tumor invasion, and induces apoptosis in GC cells. LDH-A may be a potential therapeutic target for GC.

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