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Article

Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells

  • Authors:
    • Hye-Jung Park
    • Moon-Moo Kim
  • View Affiliations / Copyright

    Affiliations: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea
  • Pages: 433-438
    |
    Published online on: November 12, 2014
       https://doi.org/10.3892/or.2014.3602
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Abstract

The aim of the present study was to examine the apoptotic effect of flavonoids in methanol extracts of Ginkgo biloba fallen leaves (MEGFL) on melanoma cells. Ginkgo biloba is a deciduous castle chaplain and its leaves include various types of flavonoids such as flavonol-O-glycosides. Ginkgo biloba is known to have therapeutic properties against a number of diseases such as cerebrovascular diseases, blood circulation disease and hypertension. In the present study MEGFL exhibited a higher cytotoxic effect on melanoma cells than Ginkgo biloba leaves (MEGL). It was also found that MEGFL induced apoptotic cell death which was characterized by DNA fragmentation. During the cell death process following treatment with MEGFL, the expression of a variety of death-associated proteins including p53, caspase-3, caspase-9, cytochrome c and Bax were analyzed in the cytosol of melanoma cells. MEGFL significantly increased the expression levels of caspase-3, caspase-9 and p53 in a dose-dependent manner. Our results indicate that MEGFL induced apoptotic cell death by increasing the expression of cell death-associated proteins in melanoma cells.
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1 

Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC and Bohr VA: Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis. 30:2–10. 2009. View Article : Google Scholar :

2 

Singhal S, Vachani A, Antin-Ozerkis D, Kaiser LR and Albelda SM: Prognostic implications of cell cycle, apoptosis, and angiogenesis biomarkers in non-small cell lung cancer: a review. Clin Cancer Res. 11:3974–3986. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Aamodt HM, Clifford A, Ellingson CC, Burnett SH, Murray BK and O’Neill KL: Apoptosis through Fas-mediated suicide gene induction in mouse melanoma. AACR. 2005:4142005.

4 

Mashima T, Naito M and Tsuruo T: Caspase-mediated cleavage of cytoskeletal actin plays a positive role in the process of morphological apoptosis. Oncogene. 18:2423–2430. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Mashima T, Naito M, Noguchi K, Miller DK, Nicholson DW and Tsuruo T: Actin cleavage by CPP-32/apopain during the development of apoptosis. Oncogene. 14:1007–1012. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Caroppi P, Sinibaldi F, Fiorucci L and Santucci R: Apoptosis and human diseases: mitochondrion damage and lethal role of released cytochrome c as proapoptotic protein. Curr Med Chem. 16:4058–4065. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Estaquier J, Vallette F, Vayssiere JL and Mignotte B: The mitochondrial pathways of apoptosis. Adv Exp Med Biol. 942:157–183. 2012. View Article : Google Scholar : PubMed/NCBI

8 

van Beek TA and Montoro P: Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J Chromatogr A. 1216:2002–2032. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Yoshikawa T, Naito Y and Kondo M: Ginkgo biloba leaf extract: review of biological actions and clinical applications. Antioxid Redox Signal. 1:469–480. 1999. View Article : Google Scholar

10 

Luo Y, Smith JV, Paramasivam V, et al: Inhibition of amyloid-beta aggregation and caspase-3 activation by the Ginkgo biloba extract EGb761. Proc Natl Acad Sci USA. 99:12197–12202. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Zhang Y, Chen AY, Li M, Chen C and Yao Q: Ginkgo biloba extract kaempferol inhibits cell proliferation and induces apoptosis in pancreatic cancer cells. J Surg Res. 148:17–23. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Waterman PG and Mole S: Analysis of Phenolic Plant Metabolites. Blackwell Scientific Publications; Oxford, UK: 1994

13 

Yen GC and Duh PD: Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J Agr Food Chem. 42:629–632. 1994. View Article : Google Scholar

14 

Imlay JA, Chin SM and Linn S: Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science. 240:640–642. 1988. View Article : Google Scholar : PubMed/NCBI

15 

Orrenius S: Apoptosis: molecular mechanisms and implications for human disease. J Intern Med. 237:529–536. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Eguchi R, Toné S, Suzuki A, et al: Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. Exp Cell Res. 315:327–335. 2009. View Article : Google Scholar

17 

Ghobrial IM, Witzig TE and Adjei AA: Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin. 55:178–194. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Martinou JC and Youle RJ: Mitochondria in apoptosis: Bcl-2 family members and mitochondrial dynamics. Dev Cell. 21:92–101. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Youle RJ and Strasser A: The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 9:47–59. 2008. View Article : Google Scholar

20 

Fan TJ, Han LH, Cong RS and Liang J: Caspase family proteases and apoptosis. Acta Biochim Biophys Sin (Shanghai). 37:719–727. 2005. View Article : Google Scholar

21 

Besson A, Dowdy SF and Roberts JM: CDK inhibitors: cell cycle regulators and beyond. Dev Cell. 14:159–169. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Levav-Cohen Y, Goldberg Z, Tan KH, Alsheich-Bartok O, Zuckerman V, Haupt S and Haupt Y: The p53-Mdm2 loop: A critical juncture of stress response. Subcell Biochem. 85:161–186. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Shieh SY, Ikeda M, Taya Y and Prives C: DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 91:325–334. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Xu J, Lian LJ, Wu C, Wang XF, Fu WY and Xu LH: Lead induces oxidative stress, DNA damage and alteration of p53, Bax and Bcl-2 expressions in mice. Food Chem Toxicol. 46:1488–1494. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Sakaguchi K, Herrera JE, Saito S, et al: DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev. 12:2831–2841. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Teufel DP, Bycroft M and Fersht AR: Regulation by phosphorylation of the relative affinities of the N-terminal transactivation domains of p53 for p300 domains and Mdm2. Oncogene. 28:2112–2118. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Fang J-Y and Lu Y-Y: Effects of histone acetylation and DNA methylation on p21WAFI regulation. World J Gastroenterol. 8:400–405. 2002.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Park H and Kim M: Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells. Oncol Rep 33: 433-438, 2015.
APA
Park, H., & Kim, M. (2015). Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells. Oncology Reports, 33, 433-438. https://doi.org/10.3892/or.2014.3602
MLA
Park, H., Kim, M."Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells". Oncology Reports 33.1 (2015): 433-438.
Chicago
Park, H., Kim, M."Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells". Oncology Reports 33, no. 1 (2015): 433-438. https://doi.org/10.3892/or.2014.3602
Copy and paste a formatted citation
x
Spandidos Publications style
Park H and Kim M: Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells. Oncol Rep 33: 433-438, 2015.
APA
Park, H., & Kim, M. (2015). Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells. Oncology Reports, 33, 433-438. https://doi.org/10.3892/or.2014.3602
MLA
Park, H., Kim, M."Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells". Oncology Reports 33.1 (2015): 433-438.
Chicago
Park, H., Kim, M."Flavonoids in Ginkgo biloba fallen leaves induce apoptosis through modulation of p53 activation in melanoma cells". Oncology Reports 33, no. 1 (2015): 433-438. https://doi.org/10.3892/or.2014.3602
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