Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
February-2015 Volume 33 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 33 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway

  • Authors:
    • Brandon M. Schickling
    • Sarah K. England
    • Nukhet Aykin-Burns
    • Lyse A. Norian
    • Kimberly K. Leslie
    • Victoria P. Frieden-Korovkina
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, University of Iowa, Iowa City, IA, USA, Division of Basic Science Research, Washington University, St. Louis, MO, USA, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, AR, USA, Department of Urology, University of Iowa, Iowa City, IA, USA
    Copyright: © Schickling et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 533-538
    |
    Published online on: November 24, 2014
       https://doi.org/10.3892/or.2014.3617
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In breast cancers, the large conductance Ca2+ and voltage sensitive K+ (BKCa) channels have been hypothesized to function as oncoproteins, yet it remains unclear how inhibition of channel activity impacts oncogenesis. We demonstrated herein that iberiotoxin (IbTX), an inhibitor of BKCa channels, differentially modulated the in vitro tumorigenic activities of hormone-independent breast cancer cells. Specifically, in HER-2/neu-overexpressing UACC893 cells and triple‑negative MDA-MB-231 cells, IbTX selectively attenuated anchorage-independent growth with concomitant downregulation of β-catenin as well as total and phosphorylated Akt and HER-2/neu. By contrast, HER-2/neu-overexpressing SK-BR-3 cells were insensitive to IbTX. Molecular analyses showed an absence of β-catenin and a dose-dependent upregulation of total and phosphorylated Akt and HER-2/neu in these cells. Taken together, these studies identify β-catenin as a putative modulator of the inhibitory actions of IbTX in sensitive breast cancer cells.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Khaitan D, Sankpal UT, Weksler B, Meister EA, Romero IA, Couraud PO and Ningaraj NS: Role of KCNMA1 gene in breast cancer invasion and metastasis to brain. BMC Cancer. 9:2582009. View Article : Google Scholar : PubMed/NCBI

2 

Tajima N, Itokazu Y, Korpi ER, Somerharju P and Käkelä R: Activity of BKCa channel is modulated by membrane cholesterol content and association with Na+/K+-ATPase in human melanoma IGR39 cells. J Biol Chem. 286:5624–5638. 2011. View Article : Google Scholar :

3 

Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, Ray A and Perera RJ: The regulation of miRNA-211 expression and its role in melanoma cell invasiveness. PLoS One. 5:e137792010. View Article : Google Scholar : PubMed/NCBI

4 

Bloch M, Ousingsawat J, Simon R, Schraml P, Gasser TC, Mihatsch MJ, Kunzelmann K and Bubendorf L: KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer. Oncogene. 26:2525–2534. 2007. View Article : Google Scholar

5 

Liu X and Chang Y, Reinhart PH, Sontheimer H and Chang Y: Cloning and characterization of glioma BK, a novel BK channel isoform highly expressed in human glioma cells. J Neurosci. 22:1840–1849. 2002.PubMed/NCBI

6 

Lu R, Alioua A, Kumar Y, Eghbali M, Stefani E and Toro L: MaxiK channel partners: physiological impact. J Physiol. 570:65–72. 2006. View Article : Google Scholar

7 

Tian L, McClafferty H, Chen L and Shipston MJ: Reversible tyrosine protein phosphorylation regulates large conductance voltage- and calcium-activated potassium channels via cortactin. J Biol Chem. 283:3067–3076. 2008. View Article : Google Scholar

8 

So EC, Wu KC, Liang CH, Chen JY and Wu SN: Evidence for activation of BKCa channels by a known inhibitor of focal adhesion kinase, PF573228. Life Sci. 89:691–701. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Sundelacruz S, Levin M and Kaplan DL: Role of membrane potential in the regulation of cell proliferation and differentiation. Stem Cell Rev. 5:231–246. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Wondergem R and Bartley JW: Menthol increases human glioblastoma intracellular Ca2+, BK channel activity and cell migration. J Biomed Sci. 16:902009. View Article : Google Scholar

11 

Arribas J, Baselga J, Pedersen K and Parra-Palau JL: p95HER2 and breast cancer. Cancer Res. 71:1515–1519. 2011. View Article : Google Scholar : PubMed/NCBI

12 

He B, You L, Uematsu K, Xu Z, Lee AY, Matsangou M, McCormick F and Jablons DM: A monoclonal antibody against Wnt-1 induces apoptosis in human cancer cells. Neoplasia. 6:7–14. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Miyoshi K and Hennighausen L: β-Catenin: a transforming actor on many stages. Breast Cancer Res. 5:63–68. 2003. View Article : Google Scholar

14 

Fang D, Hawke D, Zheng Y, Xia Y, Meisenhelder J, Nika H, Mills GB, Kobayashi R, Hunter T and Lu Z: Phosphorylation of β-catenin by AKT promotes β-catenin transcriptional activity. J Biol Chem. 282:11221–11229. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Park JH, Park SJ, Chung MK, Jung KH, Choi MR, Kim Y, Chai YG, Kim SJ and Park KS: High expression of large-conductance Ca2+-activated K+ channel in the CD133+ subpopulation of SH-SY5Y neuroblastoma cells. Biochem Biophys Res Commun. 396:637–642. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Candia S, Garcia ML and Latorre R: Mode of action of iberiotoxin, a potent blocker of the large conductance Ca(2+)-activated K+ channel. Biophys J. 63:583–590. 1992. View Article : Google Scholar : PubMed/NCBI

17 

Shimazu K, Takeda K, Yu ZX, Jiang H, Liu XW, Nelson PG and Guroff G: Multiple acute effects on the membrane potential of PC12 cells produced by nerve growth factor (NGF). J Cell Physiol. 203:501–509. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Schickling BM, Aykin-Burns N, Leslie KK, Spitz DR and Korovkina VP: An inhibitor of K+ channels modulates human endometrial tumor-initiating cells. Cancer Cell Int. 11:252011. View Article : Google Scholar

19 

Bian S, Bai JP, Chapin H, Le Moellic C, Dong H, Caplan M, Sigworth FJ and Navaratnam DS: Interactions between β-catenin and the HSlo potassium channel regulates HSlo surface expression. PLoS One. 6:e282642011. View Article : Google Scholar

20 

Schroeder JA, Adriance MC, McConnell EJ, Thompson MC, Pockaj B and Gendler SJ: ErbB-β-catenin complexes are associated with human infiltrating ductal breast and murine mammary tumor virus (MMTV)-Wnt-1 and MMTV-c-Neu transgenic carcinomas. J Biol Chem. 77:22692–22698. 2002. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Schickling BM, England SK, Aykin-Burns N, Norian LA, Leslie KK and Frieden-Korovkina VP: BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway. Oncol Rep 33: 533-538, 2015.
APA
Schickling, B.M., England, S.K., Aykin-Burns, N., Norian, L.A., Leslie, K.K., & Frieden-Korovkina, V.P. (2015). BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway. Oncology Reports, 33, 533-538. https://doi.org/10.3892/or.2014.3617
MLA
Schickling, B. M., England, S. K., Aykin-Burns, N., Norian, L. A., Leslie, K. K., Frieden-Korovkina, V. P."BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway". Oncology Reports 33.2 (2015): 533-538.
Chicago
Schickling, B. M., England, S. K., Aykin-Burns, N., Norian, L. A., Leslie, K. K., Frieden-Korovkina, V. P."BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway". Oncology Reports 33, no. 2 (2015): 533-538. https://doi.org/10.3892/or.2014.3617
Copy and paste a formatted citation
x
Spandidos Publications style
Schickling BM, England SK, Aykin-Burns N, Norian LA, Leslie KK and Frieden-Korovkina VP: BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway. Oncol Rep 33: 533-538, 2015.
APA
Schickling, B.M., England, S.K., Aykin-Burns, N., Norian, L.A., Leslie, K.K., & Frieden-Korovkina, V.P. (2015). BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway. Oncology Reports, 33, 533-538. https://doi.org/10.3892/or.2014.3617
MLA
Schickling, B. M., England, S. K., Aykin-Burns, N., Norian, L. A., Leslie, K. K., Frieden-Korovkina, V. P."BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway". Oncology Reports 33.2 (2015): 533-538.
Chicago
Schickling, B. M., England, S. K., Aykin-Burns, N., Norian, L. A., Leslie, K. K., Frieden-Korovkina, V. P."BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway". Oncology Reports 33, no. 2 (2015): 533-538. https://doi.org/10.3892/or.2014.3617
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team