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Article

The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells

  • Authors:
    • Yuan Liu
    • Wenyan Qian
    • Jiawen Zhang
    • Yu Dong
    • Can Shi
    • Zhiqiang Liu
    • Sufang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, P.R. China, Department of Gynecology and Obstetrics, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangsu, P.R. China, Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Shanghai Tongji University, Shanghai, P.R. China, Division of Cancer Medicine, Department of Lymphoma and Myeloma, Center for Cancer Immunology Research, The University of Texas MD Anderson Cancer Center, Houston, TX, USA
  • Pages: 639-650
    |
    Published online on: November 24, 2014
       https://doi.org/10.3892/or.2014.3620
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Abstract

High-risk human papillomavirus (HR-HPV) infections are among the most important factors for cervical carcinogenesis. However, whether patients infected with HR-HPV eventually develop a malignant tumor, largely depends on epithelial-mesenchymal transition (EMT), which plays an extraordinary role in the process of carcinogenesis and metastasis. Therefore, we evaluated the protein levels of EMT-related genes in normal cervical squamous epithelium, cervical intraepithelial neoplasia (CIN), and cervical squamous cell carcinoma (SCC) by tissue microarray and immunohistochemical staining. By comparing the expression of EMT-related proteins in 31 cases of cervical tumors and tumor adjacent tissues and exploring the relationship between HPV16 oncogenes and EMT in vitro, we found that Twist2 protein levels were significantly higher in CIN and cervical cancer than in normal cervical squamous epithelial samples (p<0.01 and p<0.001, respectively). This finding corresponded with the decreased expression of E-cadherin in cervical cancer. The difference in the expression of Twist2 and E-cadherin between 31 cases of cervical tumors and tumor adjacent tissues was statistically significant (p<0.01). HPV16 oncogenes were able to induce morphological alterations in the SiHa cell line, upregulate the expression of Twist2 and vimentin, downregulate E-cadherin in vitro, and exert an effect on invasion. Thus, joint detection of Twist2 and E-cadherin expression can help evaluate and provide greater insight into cervical carcinogenesis and progression.
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View References

1 

zur Hausen H: Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2:342–350. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Faridi R, Zahra A, Khan K and Idrees M: Oncogenic potential of human papillomavirus (HPV) and its relation with cervical cancer. Virol J. 8:2692011. View Article : Google Scholar : PubMed/NCBI

3 

Panjković M and Ivković-Kapicl T: Etiology and pathogenesis of precancerous lesions and invasive cervical carcinoma. Med Pregl. 61:364–368. 2008.(In Serbian). View Article : Google Scholar

4 

Snijders PJ, Steenbergen RD, Heideman DA and Meijer CJ: HPV-mediated cervical carcinogenesis: concepts and clinical implications. J Pathol. 208:152–164. 2006. View Article : Google Scholar

5 

Javier MG and Sastre-Garau X: Uterine cervix carcinoma: recent biological data and update for improving follow-up and treatment. Isr Med Assoc J. 14:700–704. 2012.PubMed/NCBI

6 

Ghittoni R, Accardi R, Hasan U, Gheit T, Sylla B and Tommasino M: The biological properties of E6 and E7 oncoproteins from human papillomaviruses. Virus Genes. 40:1–13. 2010. View Article : Google Scholar

7 

De Craene B and Berx G: Regulatory networks defining EMT during cancer initiation and progression. Nat Rev Cancer. 13:97–110. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Stewart CJ and McCluggage WG: Epithelial-mesenchymal transition in carcinomas of the female genital tract. Histopathology. 62:31–43. 2013. View Article : Google Scholar

9 

Lim J and Thiery JP: Epithelial-mesenchymal transitions: insights from development. Development. 139:3471–3486. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Le Bras GF, Taubenslag KJ and Andl CD: The regulation of cell-cell adhesion during epithelial-mesenchymal transition, motility and tumor progression. Cell Adh Migr. 6:365–373. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Rodriguez FJ, Lewis-Tuffin LJ and Anastasiadis PZ: E-cadherin’s dark side: possible role in tumor progression. Biochim Biophys Acta. 1826:23–31. 2012.PubMed/NCBI

12 

Iwatsuki M, Mimori K, Yokobori T, et al: Epithelial-mesenchymal transition in cancer development and its clinical significance. Cancer Sci. 101:293–299. 2010. View Article : Google Scholar

13 

Sanchez-Tillo E, Liu Y, de Barrios O, et al: EMT-activating transcription factors in cancer: beyond EMT and tumor invasiveness. Cell Mol Life Sci. 69:3429–3456. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lee MY and Shen MR: Epithelial-mesenchymal transition in cervical carcinoma. Am J Transl Res. 4:1–13. 2012.PubMed/NCBI

15 

Li Y, Wang W, Wang W, et al: Correlation of TWIST2 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma. Gynecol Oncol. 124:112–118. 2012. View Article : Google Scholar

16 

Chamulitrat W, Schmidt R, Chunglok W, Kohl A and Tomakidi P: Epithelium and fibroblast-like phenotypes derived from HPV16 E6/E7-immortalized human gingival keratinocytes following chronic ethanol treatment. Eur J Cell Biol. 82:313–322. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Hellner K, Mar J, Fang F, Quackenbush J and Munger K: HPV16 E7 oncogene expression in normal human epithelial cells causes molecular changes indicative of an epithelial to mesenchymal transition. Virology. 391:57–63. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Cheng YM, Chou CY, Hsu YC, Chen MJ and Wing LY: The role of human papillomavirus type 16 E6/E7 oncoproteins in cervical epithelial-mesenchymal transition and carcinogenesis. Oncol Lett. 3:667–671. 2012.PubMed/NCBI

19 

Caberg JH, Hubert PM, Begon DY, et al: Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes. Carcinogenesis. 29:1441–1447. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Mühlen S, Behren A, Iftner T and Simon C: Influence of HPV16 E2 and its localisation on the expression of matrix metalloproteinase-9. Int J Oncol. 37:337–345. 2010.PubMed/NCBI

21 

Zhang J, Yang Y, Zhang Z, et al: Gankyrin plays an essential role in estrogen-driven and GPR30-mediated endometrial carcinoma cell proliferation via the PTEN/PI3K/AKT signaling pathway. Cancer Lett. 339:279–287. 2013. View Article : Google Scholar

22 

Wang YX, Zhang XY, Zhang BF, Yang CQ and Gao HJ: Study on the clinical significance of Argonaute2 expression in colonic carcinoma by tissue microarray. Int J Clin Exp Pathol. 6:476–484. 2013.PubMed/NCBI

23 

Franco HL, Casasnovas J, Rodriguez-Medina JR and Cadilla CL: Redundant or separate entities? - roles of Twist1 and Twist2 as molecular switches during gene transcription. Nucleic Acids Res. 39:1177–1186. 2011. View Article : Google Scholar :

24 

Zeisberg M and Neilson EG: Biomarkers for epithelial-mesenchymal transitions. J Clin Invest. 119:1429–1437. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial-mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Gasparotto D, Polesel J, Marzotto A, et al: Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas. Oncotarget. 2:1165–1175. 2011.PubMed/NCBI

27 

Mao Y, Zhang N, Xu J, Ding Z, Zong R and Liu Z: Significance of heterogeneous Twist2 expression in human breast cancers. PloS One. 7:e481782012. View Article : Google Scholar : PubMed/NCBI

28 

Myong NH: Loss of E-cadherin and acquisition of vimentin in epithelial-mesenchymal transition are noble indicators of uterine cervix cancer progression. Korean J Pathol. 46:341–348. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Thiery JP and Sleeman JP: Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol. 7:131–142. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Reik W: Stability and flexibility of epigenetic gene regulation in mammalian development. Nature. 447:425–432. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Lee MY, Chou CY, Tang MJ and Shen MR: Epithelial-mesenchymal transition in cervical cancer: correlation with tumor progression, epidermal growth factor receptor overexpression, and snail up-regulation. Clin Cancer Res. 14:4743–4750. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Hsu YM, Chen YF, Chou CY, et al: KCl cotransporter-3 down-regulates E-cadherin/beta-catenin complex to promote epithelial-mesenchymal transition. Cancer Res. 67:11064–11073. 2007. View Article : Google Scholar : PubMed/NCBI

33 

van Roy F and Berx G: The cell-cell adhesion molecule E-cadherin. Cell Mol Life Sci. 65:3756–3788. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Jung YS, Kato I and Kim HR: A novel function of HPV16-E6/E7 in epithelial-mesenchymal transition. Biochem Biophys Res Commun. 435:339–344. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Watson RA, Thomas M, Banks L and Roberts S: Activity of the human papillomavirus E6 PDZ-binding motif correlates with an enhanced morphological transformation of immortalized human keratinocytes. J Cell Sci. 116:4925–4934. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Duffy CL, Phillips SL and Klingelhutz AJ: Microarray analysis identifies differentiation-associated genes regulated by human papillomavirus type 16 E6. Virology. 314:196–205. 2003. View Article : Google Scholar : PubMed/NCBI

37 

Thierry F: Transcriptional regulation of the papillomavirus oncogenes by cellular and viral transcription factors in cervical carcinoma. Virology. 384:375–379. 2009. View Article : Google Scholar

38 

Forma E, Wójcik-Krowiranda K, Bieńkiewicz A and Bryś M: Molecular basis of gynecological oncology - TopBP1 protein and its participation in the transcription process. Ginekol Pol. 83:363–367. 2012.(In Polish). PubMed/NCBI

39 

Bodily JM, Hennigan C, Wrobel GA and Rodriguez CM: Regulation of the human papillomavirus type 16 late promoter by E7 and the cell cycle. Virology. 443:11–19. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Xue Y, Lim D, Zhi L, He P, Abastado JP and Thierry F: Loss of HPV16 E2 protein expression without disruption of the E2 ORF correlates with carcinogenic progression. Open Virol J. 6:163–172. 2012. View Article : Google Scholar

41 

Ramirez-Salazar E, Centeno F, Nieto K, Valencia-Hernandez A, Salcedo M and Garrido E: HPV16 E2 could act as down-regulator in cellular genes implicated in apoptosis, proliferation and cell differentiation. Virol J. 8:2472011. View Article : Google Scholar : PubMed/NCBI

42 

Bellanger S, Tan CL, Xue YZ, Teissier S and Thierry F: Tumor suppressor or oncogene? A critical role of the human papillomavirus (HPV) E2 protein in cervical cancer progression. Am J Cancer Res. 1:373–389. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Qian W, Zhang J, Dong Y, Shi C, Liu Z and Wu S: The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells. Oncol Rep 33: 639-650, 2015.
APA
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., & Wu, S. (2015). The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells. Oncology Reports, 33, 639-650. https://doi.org/10.3892/or.2014.3620
MLA
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., Wu, S."The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells". Oncology Reports 33.2 (2015): 639-650.
Chicago
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., Wu, S."The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells". Oncology Reports 33, no. 2 (2015): 639-650. https://doi.org/10.3892/or.2014.3620
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Qian W, Zhang J, Dong Y, Shi C, Liu Z and Wu S: The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells. Oncol Rep 33: 639-650, 2015.
APA
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., & Wu, S. (2015). The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells. Oncology Reports, 33, 639-650. https://doi.org/10.3892/or.2014.3620
MLA
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., Wu, S."The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells". Oncology Reports 33.2 (2015): 639-650.
Chicago
Liu, Y., Qian, W., Zhang, J., Dong, Y., Shi, C., Liu, Z., Wu, S."The indicative function of Twist2 and E-cadherin in HPV oncogene-induced epithelial-mesenchymal transition of cervical cancer cells". Oncology Reports 33, no. 2 (2015): 639-650. https://doi.org/10.3892/or.2014.3620
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