Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
February-2015 Volume 33 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 33 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

  • Authors:
    • Zbynek Heger
    • Jaromir Gumulec
    • Natalia Cernei
    • Katerina Tmejova
    • Pavel Kopel
    • Jan Balvan
    • Michal Masarik
    • Ondrej Zitka
    • Miroslava Beklova
    • Vojtech Adam
    • Rene Kizek
  • View Affiliations / Copyright

    Affiliations: Department of Chemistry and Biochemistry, Mendel University in Brno, CZ-613 00 Brno, Czech Republic, Central European Institute of Technology, Brno University of Technology, CZ-616 00 Brno, Czech Republic, Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, CZ-612 42 Brno, Czech Republic
  • Pages: 921-929
    |
    Published online on: November 26, 2014
       https://doi.org/10.3892/or.2014.3627
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins α and β. The delivery system is composed of a cationic liposome envelope containing 17β-estradiol (E2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ERα was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ERα and β exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MT1A, NF-κB1 and K-ras genes, but not TFF1, were downregulated using E2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-κB1, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Zamecnik PC and Stephenson ML: Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligode-oxynucleotide. Proc Natl Acad Sci USA. 75:280–284. 1978. View Article : Google Scholar

2 

Warzocha K: Antisense strategy in hematological malignancies. Cytokines Cell Mol Ther. 5:15–23. 1999.PubMed/NCBI

3 

Ruden M and Puri N: Novel anticancer therapeutics targeting telomerase. Cancer Treat Rev. 39:444–456. 2013. View Article : Google Scholar

4 

Wu Y, Zhang Y, Wang M, et al: Downregulation of HER3 by a novel antisense oligonucleotide, EZN-3920, improves the antitumor activity of EGFR and HER2 tyrosine kinase inhibitors in animal models. Mol Cancer Ther. 12:427–437. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Panyam J and Labhasetwar V: Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev. 55:329–347. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Jones SE: Metastatic breast cancer: the treatment challenge. Clin Breast Cancer. 8:224–233. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Xie QC, Hu YD, Wang LL, et al: The co-transfection of p16(INK4a) and p14(ARF) genes into human lung cancer cell line A549 and the effects on cell growth and chemosensitivity. Colloids Surf B Biointerfaces. 46:188–196. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Stein CA: Two problems in antisense biotechnology: in vitro delivery and the design of antisense experiments. Biochim Biophys Acta. 1489:45–52. 1999. View Article : Google Scholar

9 

Balbino TA, Azzoni AR and de la Torre LG: Microfluidic devices for continuous production of pDNA/cationic liposome complexes for gene delivery and vaccine therapy. Colloid Surf B Biointerfaces. 111:203–210. 2013. View Article : Google Scholar

10 

Skoblov Mlu: Prospects of antisense therapy technologies. Mol Biol. 43:984–998. 2009.(In Russian). View Article : Google Scholar

11 

Zhong J, Yao X, Li DL, et al: Large scale preparation of midkine antisense oligonucleotides nanoliposomes by a cross-flow injection technique combined with ultrafiltration and high-pressure extrusion procedures. Int J Pharm. 441:712–720. 2013. View Article : Google Scholar

12 

Ye S, Yang W, Wang Y, et al: Cationic liposome-mediated nitric oxide synthase gene therapy enhances the antitumor effects of cisplatin in lung cancer. Int J Mol Med. 31:33–42. 2013.

13 

Kim TI and Kim SW: Bioreducible polymers for gene delivery. React Funct Polym. 71:344–349. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Xu M, Kumar D, Srinivas S, et al: Parenteral gene therapy with p53 inhibits human breast tumors in vivo through a bystander mechanism without evidence of toxicity. Hum Gene Ther. 8:177–185. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Nakase M, Inui M, Okumura K, Kamei T, Nakamura S and Tagawa T: p53 gene therapy of human osteosarcoma using a transferrin-modified cationic liposome. Mol Cancer Ther. 4:625–631. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Krejcova L, Hynek D, Kopel P, et al: Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene. Viruses. 5:1719–1739. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Kensova R, Kremplova M, Smerkova K, et al: Interactions of platinum-based cytostatics with metallothionein revealed by electrochemistry. Int J Electrochem Sci. 8:4472–4484. 2013.

18 

Krystofova O, Sochor J, Zitka O, et al: Effect of magnetic nanoparticles on tobacco BY-2 cell suspension culture. Int J Environ Res Public Health. 10:47–71. 2012. View Article : Google Scholar

19 

Misra SK, Naz S, Kondaiah P and Bhattacharya S: A cationic cholesterol based nanocarrier for the delivery of p53-EGFP-C3 plasmid to cancer cells. Biomaterials. 35:1334–1346. 2014. View Article : Google Scholar

20 

Taylor AH, Pringle JH, Bell SC and Al-Azzawi F: Specific inhibition of estrogen receptor alpha function by antisense oligodeoxyribonucleotides. Antisense Nucleic Acid Drug Dev. 11:219–231. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Ruyra A, Cano-Sarabia M, Mackenzie SA, Maspoch D and Roher N: A novel liposome-based nanocarrier loaded with an LPS-dsRNA cocktail for fish innate immune system stimulation. PLoS One. 8:e763382013. View Article : Google Scholar : PubMed/NCBI

22 

Dinu-Pirvu C, Ferdes M, Butu A, Ortan A and Ghica MV: Physicochemical investigation of low soluble biocompounds entrapped in lipid carriers. Farmacia. 61:182–192. 2013.

23 

Lacroix M and Leclercq G: Relevance of breast cancer cell lines as models for breast tumours: an update. Breast Cancer Res Treat. 83:249–289. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Resende FA, de Oliveira AP, de Camargo MS, Vilegas W and Varanda EA: Evaluation of estrogenic potential of flavonoids using a recombinant yeast strain and MCF7/BUS cell proliferation assay. PLoS One. 8:e748812013. View Article : Google Scholar : PubMed/NCBI

25 

Hu ZZ, Kagan BL, Ariazi EA, et al: Proteomic analysis of pathways involved in estrogen-induced growth and apoptosis of breast cancer cells. PLoS One. 6:e204102011. View Article : Google Scholar : PubMed/NCBI

26 

Yakimchuk K, Jondal M and Okret S: Estrogen receptor α and β in the normal immune system and in lymphoid malignancies. Mol Cell Endocrinol. 375:121–129. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Tu ZZ, Ma Y, Tian J, et al: Estrogen receptor β potentiates the antiproliferative effect of raloxifene and affects the cell migration and invasion in HCT-116 colon cancer cells. J Cancer Res Clin Oncol. 138:1091–1103. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Li H, Tu Z, An L, Qian Z, Achilefu S and Gu Y: Inhibitory effects of ERβ on proliferation, invasion, and tumor formation of MCF-7 breast cancer cells - prognostication for the use of ERβ-selective therapy. Pharm Biol. 50:839–849. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Hartman J, Lindberg K, Morani A, Inzunza J, Strom A and Gustafsson JA: Estrogen receptor beta inhibits angiogenesis and growth of T47D breast cancer xenografts. Cancer Res. 66:11207–11213. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Costa DD, Neto FF, Costa MD, et al: Vitellogenesis and other physiological responses induced by 17-beta-estradiol in males of freshwater fish Rhamdia quelen. Comp Biochem Physiol C Toxicol Pharmacol. 151:248–257. 2010. View Article : Google Scholar

31 

Woo S, Won H, Lee A and Yum S: Oxidative stress and gene expression in diverse tissues of Oryzias javanicus exposed to 17β-estradiol. Mol Cell Toxicol. 8:263–269. 2012. View Article : Google Scholar

32 

Bhat HK, Calaf G, Hei TK, Loya T and Vadgama JV: Critical role of oxidative stress in estrogen-induced carcinogenesis. Proc Natl Acad Sci USA. 100:3913–3918. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Li W, Jia M, Qin X, Hu J, Zhang X and Zhou G: Harmful effect of ERβ on BCRP-mediated drug resistance and cell proliferation in ERα/PR-negative breast cancer. FEBS J. 280:6128–6140. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Garg A and Aggarwal BB: Nuclear transcription factor-kappaB as a target for cancer drug development. Leukemia. 16:1053–1068. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Chen F, Castranova V, Shi X and Demers LM: New insights into the role of nuclear factor-kappaB, a ubiquitous transcription factor in the initiation of diseases. Clin Chem. 45:7–17. 1999.PubMed/NCBI

36 

Kato K, Horiuchi S, Takahashi A, et al: Contribution of estrogen receptor alpha to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway. J Biol Chem. 277:11217–11224. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Tu Z, Gui L, Wang J, Li X, Sun P and Wei L: Tumorigenesis of K-ras mutation in human endometrial carcinoma via upregulation of estrogen receptor. Gynecol Oncol. 101:274–279. 2006. View Article : Google Scholar

38 

Buache E, Etique N, Alpy F, et al: Deficiency in trefoil factor 1 (TFF1) increases tumorigenicity of human breast cancer cells and mammary tumor development in TFF1-knockout mice. Oncogene. 30:3261–3273. 2011. View Article : Google Scholar : PubMed/NCBI

39 

May FE and Westley BR: Trefoil proteins: their role in normal and malignant cells. J Pathol. 183:4–7. 1997. View Article : Google Scholar : PubMed/NCBI

40 

Corte MD, Tamargo F, Alvarez A, et al: Cytosolic levels of TFF1/pS2 in breast cancer: their relationship with clinical-pathological parameters and their prognostic significance. Breast Cancer Res Treat. 96:63–72. 2006. View Article : Google Scholar

41 

Surowiak P, Matkowski R, Materna V, et al: Elevated metallothionein (MT) expression in invasive ductal breast cancers predicts tamoxifen resistance. Histol Histopathol. 20:1037–1044. 2005.PubMed/NCBI

42 

Markicevic M, Petrovic A, Kanjer K, Neskovic-Konstantinovic Z and Nikolic-Vukosavujevic D: Estrogen-regulated cut-off values of pS2 and cathepsin D expression in breast carcinomas. Adv Exp Med Biol. 617:341–348. 2008. View Article : Google Scholar : PubMed/NCBI

43 

Gorrini C, Harris IS and Mak TW: Modulation of oxidative stress as an anticancer strategy. Nat Rev Drug Discov. 12:931–947. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Traverso N, Ricciarelli R, Nitti M, et al: Role of glutathione in cancer progression and chemoresistance. Oxid Med Cell Longev. 2013:9729132013. View Article : Google Scholar : PubMed/NCBI

45 

Hishikawa Y, Abe S, Kinugasa S, et al: Overexpression of metallothionein correlates with chemoresistance to cisplatin and prognosis in esophageal cancer. Oncology. 54:342–347. 1997. View Article : Google Scholar : PubMed/NCBI

46 

Endo T, Yoshikawa M, Ebara M, et al: Immunohistochemical metallothionein expression in hepatocellular carcinoma: relation to tumor progression and chemoresistance to platinum agents. J Gastroenterol. 39:1196–1201. 2004. View Article : Google Scholar : PubMed/NCBI

47 

Yap X, Tan HY, Huang J, et al: Over-expression of metallothionein predicts chemoresistance in breast cancer. J Pathol. 217:563–570. 2009. View Article : Google Scholar : PubMed/NCBI

48 

Kizek R, Adam V, Hrabeta J, et al: Anthracyclines and ellipticines as DNA-damaging anticancer drugs: recent advances. Pharmacol Ther. 133:26–39. 2012. View Article : Google Scholar

49 

Klein S, Dell’Arciprete ML, Wegmann M, et al: Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells. Biochem Biophys Res Commun. 434:217–222. 2013. View Article : Google Scholar : PubMed/NCBI

50 

Marnett LJ: Oxy radicals, lipid peroxidation and DNA damage. Toxicology. 181–182:219–222. 2002. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Heger Z, Gumulec J, Cernei N, Tmejova K, Kopel P, Balvan J, Masarik M, Zitka O, Beklova M, Adam V, Adam V, et al: 17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. Oncol Rep 33: 921-929, 2015.
APA
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J. ... Kizek, R. (2015). 17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. Oncology Reports, 33, 921-929. https://doi.org/10.3892/or.2014.3627
MLA
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J., Masarik, M., Zitka, O., Beklova, M., Adam, V., Kizek, R."17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line". Oncology Reports 33.2 (2015): 921-929.
Chicago
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J., Masarik, M., Zitka, O., Beklova, M., Adam, V., Kizek, R."17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line". Oncology Reports 33, no. 2 (2015): 921-929. https://doi.org/10.3892/or.2014.3627
Copy and paste a formatted citation
x
Spandidos Publications style
Heger Z, Gumulec J, Cernei N, Tmejova K, Kopel P, Balvan J, Masarik M, Zitka O, Beklova M, Adam V, Adam V, et al: 17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. Oncol Rep 33: 921-929, 2015.
APA
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J. ... Kizek, R. (2015). 17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. Oncology Reports, 33, 921-929. https://doi.org/10.3892/or.2014.3627
MLA
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J., Masarik, M., Zitka, O., Beklova, M., Adam, V., Kizek, R."17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line". Oncology Reports 33.2 (2015): 921-929.
Chicago
Heger, Z., Gumulec, J., Cernei, N., Tmejova, K., Kopel, P., Balvan, J., Masarik, M., Zitka, O., Beklova, M., Adam, V., Kizek, R."17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line". Oncology Reports 33, no. 2 (2015): 921-929. https://doi.org/10.3892/or.2014.3627
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team