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Article

Sister chromatid exchange: A possible approach to characterize familial breast cancer patients

  • Authors:
    • Ivana De Pascalis
    • Brunella Pilato
    • Annalisa Mazzotta
    • Teresa Stefania Dell'Endice
    • Vincenza Rubini
    • Giovanni Simone
    • Angelo Paradiso
    • Vincenzo Aiello
    • Anita Mangia
  • View Affiliations / Copyright

    Affiliations: Functional Biomorphology Laboratory, NCRC, IRCSS Istituto Tumori ‘Giovanni Paolo II’, Bari, Italy, Molecular Genetics Laboratory, NCRC, IRCSS Istituto Tumori ‘Giovanni Paolo II’, Bari, Italy, Pathology Department, NCRC, IRCSS Istituto Tumori ‘Giovanni Paolo II’, Bari, Italy, Experimental Medical Oncology, NCRC, IRCSS Istituto Tumori ‘Giovanni Paolo II’, Bari, Italy, Medical Genetics, University of Ferrara, Ferrara, Italy
  • Pages: 930-934
    |
    Published online on: November 26, 2014
       https://doi.org/10.3892/or.2014.3628
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Abstract

Sister chromatid exchange (SCE) frequency is widely used as an indicator of spontaneous chromosome instability. We investigated SCE frequency in the peripheral blood lymphocytes of familial and sporadic breast cancer (BC) patients from the Apulian Caucasian Population. Eighty-one patients were enrolled: 22 with familial history and 59 sporadic patients. Eleven familial patients had an ‘increased risk’ of BRCA gene mutation (BRCAPro ≥10%) and were candidates for BRCA1 and BRCA2 mutation analysis. For these reasons, we stratified the 22 familial BC patients in two group: ‘low-risk’ (n=11) and ‘high-risk’ (n=11) patients for BRCA gene mutations. Two of these 11 ‘high-risk’ patients (18%) had pathogenic mutations in the BRCA2 gene. The subjects were not cigarette smokers or alcohol or drug users, and had no genetic disorders or chronic diseases affecting the family. Our results showed a significant increase in SCE frequency in the familial (5.305±1.088/metaphase) (P<0.0001) and the sporadic patients (3.943±0.552) (P<0.0001) compared to the controls (3.197±0.649). We found that the SCE frequency was always significantly higher in familial than in sporadic patients, regardless of their clinicopathological characteristics. Moreover, we observed that the frequency of SCE in BRCA2 mutation carrier patients was higher compared to patients without mutations in BRCA1/2 genes. These findings highlight an intrinsic genomic instability in familial patients, and we suggest that SCE frequency may be used as a biomarker to better characterize familial BC.
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Copy and paste a formatted citation
Spandidos Publications style
De Pascalis I, Pilato B, Mazzotta A, Dell'Endice TS, Rubini V, Simone G, Paradiso A, Aiello V and Mangia A: Sister chromatid exchange: A possible approach to characterize familial breast cancer patients. Oncol Rep 33: 930-934, 2015.
APA
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T.S., Rubini, V., Simone, G. ... Mangia, A. (2015). Sister chromatid exchange: A possible approach to characterize familial breast cancer patients. Oncology Reports, 33, 930-934. https://doi.org/10.3892/or.2014.3628
MLA
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T. S., Rubini, V., Simone, G., Paradiso, A., Aiello, V., Mangia, A."Sister chromatid exchange: A possible approach to characterize familial breast cancer patients". Oncology Reports 33.2 (2015): 930-934.
Chicago
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T. S., Rubini, V., Simone, G., Paradiso, A., Aiello, V., Mangia, A."Sister chromatid exchange: A possible approach to characterize familial breast cancer patients". Oncology Reports 33, no. 2 (2015): 930-934. https://doi.org/10.3892/or.2014.3628
Copy and paste a formatted citation
x
Spandidos Publications style
De Pascalis I, Pilato B, Mazzotta A, Dell'Endice TS, Rubini V, Simone G, Paradiso A, Aiello V and Mangia A: Sister chromatid exchange: A possible approach to characterize familial breast cancer patients. Oncol Rep 33: 930-934, 2015.
APA
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T.S., Rubini, V., Simone, G. ... Mangia, A. (2015). Sister chromatid exchange: A possible approach to characterize familial breast cancer patients. Oncology Reports, 33, 930-934. https://doi.org/10.3892/or.2014.3628
MLA
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T. S., Rubini, V., Simone, G., Paradiso, A., Aiello, V., Mangia, A."Sister chromatid exchange: A possible approach to characterize familial breast cancer patients". Oncology Reports 33.2 (2015): 930-934.
Chicago
De Pascalis, I., Pilato, B., Mazzotta, A., Dell'Endice, T. S., Rubini, V., Simone, G., Paradiso, A., Aiello, V., Mangia, A."Sister chromatid exchange: A possible approach to characterize familial breast cancer patients". Oncology Reports 33, no. 2 (2015): 930-934. https://doi.org/10.3892/or.2014.3628
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