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Article

Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model

  • Authors:
    • Tatsuya Ando
    • Hiroyasu Ito
    • Yuko Arioka
    • Hideyuki Ogiso
    • Mitsuru Seishima
  • View Affiliations / Copyright

    Affiliations: Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
  • Pages: 826-832
    |
    Published online on: November 28, 2014
       https://doi.org/10.3892/or.2014.3634
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Abstract

α-galactosylceramide (GalCer), which is a natural killer T (NKT) cell ligand, has been reported to exert therapeutic effects against cancer in humans and mice. Toll-like receptor (TLR) agonists systemically or locally boost antitumor efficacy in mouse cancer models. In our previous study, the co-administration of GalCer and a TLR agonist synergistically enhanced interferon-γ (IFN-γ) production in mouse splenocytes in vitro and in vivo. The increased IFN-γ production promoted a tumor antigen-specific Th1 response. Therefore, co-treatment with GalCer and a TLR agonist is expected to exert an enhanced antitumor effect. In the present study, we examined the effect of GalCer and lipopolysaccharide (LPS) combination therapy in a mouse lung-metastasis model. GalCer and LPS combination therapy markedly decreased the number of lung metastatic tumor nodes. Co-treatment with GalCer and LPS enhanced the mRNA expression of CXCL9 and CXCL10 in mediastinal lymph nodes (MLNs) and increased the number of CD8+ cells in the MLNs. Furthermore, the depletion of CD8+ T cells canceled the antitumor effect of GalCer and LPS combination therapy. Thus, GalCer and LPS combination therapy significantly enhanced tumor antigen-specific immune responses and suppressed tumor growth in a mouse lung-metastasis model.
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Copy and paste a formatted citation
Spandidos Publications style
Ando T, Ito H, Arioka Y, Ogiso H and Seishima M: Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model. Oncol Rep 33: 826-832, 2015.
APA
Ando, T., Ito, H., Arioka, Y., Ogiso, H., & Seishima, M. (2015). Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model. Oncology Reports, 33, 826-832. https://doi.org/10.3892/or.2014.3634
MLA
Ando, T., Ito, H., Arioka, Y., Ogiso, H., Seishima, M."Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model". Oncology Reports 33.2 (2015): 826-832.
Chicago
Ando, T., Ito, H., Arioka, Y., Ogiso, H., Seishima, M."Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model". Oncology Reports 33, no. 2 (2015): 826-832. https://doi.org/10.3892/or.2014.3634
Copy and paste a formatted citation
x
Spandidos Publications style
Ando T, Ito H, Arioka Y, Ogiso H and Seishima M: Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model. Oncol Rep 33: 826-832, 2015.
APA
Ando, T., Ito, H., Arioka, Y., Ogiso, H., & Seishima, M. (2015). Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model. Oncology Reports, 33, 826-832. https://doi.org/10.3892/or.2014.3634
MLA
Ando, T., Ito, H., Arioka, Y., Ogiso, H., Seishima, M."Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model". Oncology Reports 33.2 (2015): 826-832.
Chicago
Ando, T., Ito, H., Arioka, Y., Ogiso, H., Seishima, M."Combination therapy with α-galactosylceramide and a Toll-like receptor agonist exerts an augmented suppressive effect on lung tumor metastasis in a mouse model". Oncology Reports 33, no. 2 (2015): 826-832. https://doi.org/10.3892/or.2014.3634
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