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Article

Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice

  • Authors:
    • Jun Zhang
    • Jianmin Yang
    • Yuan Cai
    • Ni Jin
    • Huiju Wang
    • Tong Yu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Gastroenterology, Sir Run Run Shaw Hospital, Hangzhou, Zhejiang 310016, P.R. China, Department of Intensive Care Medicine, The Central Hospital of Jinhua City, Jinhua, Zhejiang 321001, P.R. China
  • Pages: 1248-1256
    |
    Published online on: December 18, 2014
       https://doi.org/10.3892/or.2014.3679
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Abstract

The purpose of this study was to evaluate the anti‑growth effect of the self‑designed multiple antigenic polypeptide (MAP) vaccine comprising B‑cell epitopes of heparanase (HPSE) on HCC97‑H hepatocellular carcinoma (HCC) in mice. The polyclonal antibodies against the B‑cell epitopes of HPSE were prepared by immunizing rabbits with freshly synthesized MAP vaccine. HCC‑bearing models were constructed on BALB/c nude mice. Anti‑MAP antibodies were administrered to the models to assess the effects on HPSE activity, HCC growth, the expression of VEGF/bFGF and the value of micro‑vessel density (MVD). The anti‑MAP antibodies were harvested, purified and identified. These antibodies were able to specifically bind with the dominant epitopes of the precursor protein and large subunit monomer of HPSE, decrease HPSE activity, suppress the expressions of VEGF and bFGF, reduce the MVD, and markedly shrink the HCC volume. Based on these findings, MAP vaccine based on the B‑cell epitopes of HPSE seemed to provide theoretical evidence for further study of the synthesized HPSE MAP vaccine in the treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Yang J, Cai Y, Jin N, Wang H and Yu T: Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice. Oncol Rep 33: 1248-1256, 2015.
APA
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., & Yu, T. (2015). Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice. Oncology Reports, 33, 1248-1256. https://doi.org/10.3892/or.2014.3679
MLA
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., Yu, T."Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice". Oncology Reports 33.3 (2015): 1248-1256.
Chicago
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., Yu, T."Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice". Oncology Reports 33, no. 3 (2015): 1248-1256. https://doi.org/10.3892/or.2014.3679
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Yang J, Cai Y, Jin N, Wang H and Yu T: Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice. Oncol Rep 33: 1248-1256, 2015.
APA
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., & Yu, T. (2015). Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice. Oncology Reports, 33, 1248-1256. https://doi.org/10.3892/or.2014.3679
MLA
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., Yu, T."Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice". Oncology Reports 33.3 (2015): 1248-1256.
Chicago
Zhang, J., Yang, J., Cai, Y., Jin, N., Wang, H., Yu, T."Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice". Oncology Reports 33, no. 3 (2015): 1248-1256. https://doi.org/10.3892/or.2014.3679
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