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Article

Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin

Retraction in: /10.3892/or.2021.8213
  • Authors:
    • Yang Qu
    • Peng Xia
    • Shanyong Zhang
    • Su Pan
    • Jianwu Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130042, P.R. China
  • Pages: 1177-1184
    |
    Published online on: December 23, 2014
       https://doi.org/10.3892/or.2014.3698
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Abstract

X-chromosome-linked inhibitor of apoptosis protein (XIAP) is an important member of the inhibitors of apoptosis (IAP) family. It has been shown that XIAP promotes the invasion, metastasis, growth and survival of malignant cells, and confers resistance to some chemotherapeutic drugs in various types of cancer. However, little is known regarding its detailed role in osteosarcoma (OS). In the present study, we first investigated the expression of XIAP in OS tissues, and an increased expression of XIAP in OS tissues compared to adjacent non-tumor tissue was identified. Additionally, its expression level correlated with key pathological characteristics including clinical stage, tumor size and metastasis. Subsequently, small interfering RNA (siRNA) was used to block XIAP expression to evaluate the effect of XIAP siRNA on cell proliferation, colony formation, cell cycle, apoptosis, tumorigenicity, and the combined effects of doxorubicin or cisplatin in OS cell lines (MG63 cells). Downregulation of XIAP expression using the RNA silencing approach efficiently decreased cell proliferation and colony formation, and induced cell apoptosis and cell cycle in the G0/G1 stage. In addition, this downregulation inhibited tumor growth in a nude murine model. The resuls also showed that treatment with XIAP-shRNA in combination with doxorubicin or cisplatin enhanced chemosensitivity. These results suggested that XIAP may aid the diagnosis of OS and may be an effective strategy for gene therapy of this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Qu Y, Xia P, Zhang S, Pan S and Zhao J: Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213. Oncol Rep 33: 1177-1184, 2015.
APA
Qu, Y., Xia, P., Zhang, S., Pan, S., & Zhao, J. (2015). Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213. Oncology Reports, 33, 1177-1184. https://doi.org/10.3892/or.2014.3698
MLA
Qu, Y., Xia, P., Zhang, S., Pan, S., Zhao, J."Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213". Oncology Reports 33.3 (2015): 1177-1184.
Chicago
Qu, Y., Xia, P., Zhang, S., Pan, S., Zhao, J."Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213". Oncology Reports 33, no. 3 (2015): 1177-1184. https://doi.org/10.3892/or.2014.3698
Copy and paste a formatted citation
x
Spandidos Publications style
Qu Y, Xia P, Zhang S, Pan S and Zhao J: Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213. Oncol Rep 33: 1177-1184, 2015.
APA
Qu, Y., Xia, P., Zhang, S., Pan, S., & Zhao, J. (2015). Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213. Oncology Reports, 33, 1177-1184. https://doi.org/10.3892/or.2014.3698
MLA
Qu, Y., Xia, P., Zhang, S., Pan, S., Zhao, J."Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213". Oncology Reports 33.3 (2015): 1177-1184.
Chicago
Qu, Y., Xia, P., Zhang, S., Pan, S., Zhao, J."Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin Retraction in /10.3892/or.2021.8213". Oncology Reports 33, no. 3 (2015): 1177-1184. https://doi.org/10.3892/or.2014.3698
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