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Article

MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1

  • Authors:
    • Xin Sun
    • Bin Liu
    • Xu-Dong Zhao
    • Li-Yin Wang
    • Wen-Yue Ji
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China
  • Pages: 1221-1226
    |
    Published online on: January 13, 2015
       https://doi.org/10.3892/or.2015.3714
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Abstract

Laryngeal cancer is one of the most commonly occurring malignant cancers of the head and neck region. In the present study, we investigated the roles of miR-221 in laryngeal squamous cell carcinoma cell line, Hep-2. We examined the function and mechanism of miR-221 in Hep-2 cells using techniques of cell biology and molecular pathology, such as western blotting, quantitative PCR, immunohistochemical staining and flow cytometry. Using a luciferase assay, the apoptotic protease activating factor-1 (Apaf-1) mRNA 3'-UTR was shown to have complementary binding sites using bioinformatics prediction software including TargetScan, PicTar and miRanda. In conclusion, our results showed that miR-221 inhibition caused elevated expression levels of the Apaf-1 apoptotic pathway proteins caspase-3, -8 and -9. miR-221 may therefore be used as a novel therapeutic target for laryngeal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sun X, Liu B, Zhao X, Wang L and Ji W: MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1. Oncol Rep 33: 1221-1226, 2015.
APA
Sun, X., Liu, B., Zhao, X., Wang, L., & Ji, W. (2015). MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1. Oncology Reports, 33, 1221-1226. https://doi.org/10.3892/or.2015.3714
MLA
Sun, X., Liu, B., Zhao, X., Wang, L., Ji, W."MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1". Oncology Reports 33.3 (2015): 1221-1226.
Chicago
Sun, X., Liu, B., Zhao, X., Wang, L., Ji, W."MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1". Oncology Reports 33, no. 3 (2015): 1221-1226. https://doi.org/10.3892/or.2015.3714
Copy and paste a formatted citation
x
Spandidos Publications style
Sun X, Liu B, Zhao X, Wang L and Ji W: MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1. Oncol Rep 33: 1221-1226, 2015.
APA
Sun, X., Liu, B., Zhao, X., Wang, L., & Ji, W. (2015). MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1. Oncology Reports, 33, 1221-1226. https://doi.org/10.3892/or.2015.3714
MLA
Sun, X., Liu, B., Zhao, X., Wang, L., Ji, W."MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1". Oncology Reports 33.3 (2015): 1221-1226.
Chicago
Sun, X., Liu, B., Zhao, X., Wang, L., Ji, W."MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1". Oncology Reports 33, no. 3 (2015): 1221-1226. https://doi.org/10.3892/or.2015.3714
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