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Article

microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer

  • Authors:
    • Xiao-Wei Wang
    • Xue-Qin Xi
    • Jian Wu
    • Yi-Yuan Wan
    • Hong-Xia Hui
    • Xiu-Feng Cao
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Clinical Laboratory, Huai'an Hospital Affiliated to Xuzhou Medical College, Huai'an, Jiangsu 223002, P.R. China, Department of Pathology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Surgical Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China
  • Pages: 1402-1410
    |
    Published online on: January 19, 2015
       https://doi.org/10.3892/or.2015.3731
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Abstract

Colorectal cancer (CRC) is the most common cancer diagnosed worldwide, and the development of metastases is a major cause of mortality. Accumulating evidence suggests that microRNAs are important in carcinogenesis by affecting the expression of genes that regulate cancer progression. A number of studies have shown that miR-206 is frequently downregulated in many human malignancies, including CRC, and is associated with a more malignant phenotype. Previous studies involving HeLa and C2C12 cells have validated the inhibitory mechanism of miR-206 via NOTCH3 targeting. However, whether or not the interplay between miR-206 and NOTCH3 also occurs in CRC is unknown. Therefore, we investigated the tumor suppressive and metastatic effects of miR-206 and its target, NOTCH3, in CRC. Based on the inverse association between the expression of miR-206 and NOTCH3 in CRC tissues, miR-206 mimics were transiently transfected into the SW480 (and its metastatic strain) and SW620 colon cancer cell lines. Upregulation of miR-206 inhibited cancer cell prolife­ration and migration, blocked the cell cycle, and activated apoptosis. The tumor suppressive capacity of miR-206 had a similar effect on CRC cells, although with a different metastatic potential, and may be explained by direct NOTCH3 signaling inhibition and indirect cross-talk with other signaling pathways involving CDH2 and MMP-9. These results support miR-206 as a tumor suppressor in CRC and suggest a potential therapeutic target for clinical intervention.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Xi X, Wu J, Wan Y, Hui H and Cao X: microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncol Rep 33: 1402-1410, 2015.
APA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., & Cao, X. (2015). microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncology Reports, 33, 1402-1410. https://doi.org/10.3892/or.2015.3731
MLA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33.3 (2015): 1402-1410.
Chicago
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33, no. 3 (2015): 1402-1410. https://doi.org/10.3892/or.2015.3731
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Xi X, Wu J, Wan Y, Hui H and Cao X: microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncol Rep 33: 1402-1410, 2015.
APA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., & Cao, X. (2015). microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncology Reports, 33, 1402-1410. https://doi.org/10.3892/or.2015.3731
MLA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33.3 (2015): 1402-1410.
Chicago
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33, no. 3 (2015): 1402-1410. https://doi.org/10.3892/or.2015.3731
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