microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer

  • Authors:
    • Xiao-Wei Wang
    • Xue-Qin Xi
    • Jian Wu
    • Yi-Yuan Wan
    • Hong-Xia Hui
    • Xiu-Feng Cao
  • View Affiliations

  • Published online on: January 19, 2015     https://doi.org/10.3892/or.2015.3731
  • Pages: 1402-1410
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Abstract

Colorectal cancer (CRC) is the most common cancer diagnosed worldwide, and the development of metastases is a major cause of mortality. Accumulating evidence suggests that microRNAs are important in carcinogenesis by affecting the expression of genes that regulate cancer progression. A number of studies have shown that miR-206 is frequently downregulated in many human malignancies, including CRC, and is associated with a more malignant phenotype. Previous studies involving HeLa and C2C12 cells have validated the inhibitory mechanism of miR-206 via NOTCH3 targeting. However, whether or not the interplay between miR-206 and NOTCH3 also occurs in CRC is unknown. Therefore, we investigated the tumor suppressive and metastatic effects of miR-206 and its target, NOTCH3, in CRC. Based on the inverse association between the expression of miR-206 and NOTCH3 in CRC tissues, miR-206 mimics were transiently transfected into the SW480 (and its metastatic strain) and SW620 colon cancer cell lines. Upregulation of miR-206 inhibited cancer cell prolife­ration and migration, blocked the cell cycle, and activated apoptosis. The tumor suppressive capacity of miR-206 had a similar effect on CRC cells, although with a different metastatic potential, and may be explained by direct NOTCH3 signaling inhibition and indirect cross-talk with other signaling pathways involving CDH2 and MMP-9. These results support miR-206 as a tumor suppressor in CRC and suggest a potential therapeutic target for clinical intervention.
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March-2015
Volume 33 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang X, Xi X, Wu J, Wan Y, Hui H and Cao X: microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncol Rep 33: 1402-1410, 2015
APA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., & Cao, X. (2015). microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer. Oncology Reports, 33, 1402-1410. https://doi.org/10.3892/or.2015.3731
MLA
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33.3 (2015): 1402-1410.
Chicago
Wang, X., Xi, X., Wu, J., Wan, Y., Hui, H., Cao, X."microRNA-206 attenuates tumor proliferation and migration involving the downregulation of NOTCH3 in colorectal cancer". Oncology Reports 33, no. 3 (2015): 1402-1410. https://doi.org/10.3892/or.2015.3731