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Article

CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway

  • Authors:
    • Hongbin Wang
    • Li Wei
    • Changli Li
    • Jing Zhou
    • Zhigao Li
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 1089-1096
    |
    Published online on: January 20, 2015
       https://doi.org/10.3892/or.2015.3736
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Abstract

Cyclin-dependent kinase 5 regulatory subunit associated protein 1 (CDK5RAP1) is an enzyme which post-synthetically converts the RNA modification N6-iso-pentenyladenosine (i6A) into 2-methylthio-N6-isopentenyladenosine (ms2i6A). However, the interaction between CDK5RAP1 deficiency and cell apoptosis has not been studied. Breast cancer has long been a leading cause of mortality in the world. Therefore, in the present study, CDK5RAP1 deficiency in a human breast cancer cell line was investigated. CDK5RAP1 small interfering RNA (siRNA) and negative control siRNA were transfected into MCF-7 cells, and the cells were further incubated for 48 h. CDK5RAP1 deficiency suppressed tumor growth in MCF-7 cells and arrested the cells at G2/M phase. CDK5RAP1 deficiency also induced cell apoptosis and reactive oxygen species (ROS) generation. Furthermore, western blot analysis showed that the expression of phospho-c-Jun N-terminal kinase (p-JNK), p53, caspase-9 and caspase-3 were upregulated in CDK5RAP1-deficient MCF-7 cells. Pretreatment with N-acetyl-cysteine (NAC), the inhibitor of ROS, or with SP600125, the inhibitor of JNK, prevented the apoptosis and the high expression of p-JNK, p53, caspase-9 and caspase-3 in CDK5RAP1-deficient MCF-7 cells. Taken together, these data indicated that CDK5RAP1 deficiency induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells by the ROS/JNK signaling pathway. Our findings indicated a novel therapeutic strategy for cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Wei L, Li C, Zhou J and Li Z: CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway. Oncol Rep 33: 1089-1096, 2015.
APA
Wang, H., Wei, L., Li, C., Zhou, J., & Li, Z. (2015). CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway. Oncology Reports, 33, 1089-1096. https://doi.org/10.3892/or.2015.3736
MLA
Wang, H., Wei, L., Li, C., Zhou, J., Li, Z."CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway". Oncology Reports 33.3 (2015): 1089-1096.
Chicago
Wang, H., Wei, L., Li, C., Zhou, J., Li, Z."CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway". Oncology Reports 33, no. 3 (2015): 1089-1096. https://doi.org/10.3892/or.2015.3736
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Wei L, Li C, Zhou J and Li Z: CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway. Oncol Rep 33: 1089-1096, 2015.
APA
Wang, H., Wei, L., Li, C., Zhou, J., & Li, Z. (2015). CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway. Oncology Reports, 33, 1089-1096. https://doi.org/10.3892/or.2015.3736
MLA
Wang, H., Wei, L., Li, C., Zhou, J., Li, Z."CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway". Oncology Reports 33.3 (2015): 1089-1096.
Chicago
Wang, H., Wei, L., Li, C., Zhou, J., Li, Z."CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway". Oncology Reports 33, no. 3 (2015): 1089-1096. https://doi.org/10.3892/or.2015.3736
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