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Article

Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells

  • Authors:
    • Sergej Telentschak
    • Mark Soliwoda
    • Klaus Nohroudi
    • Klaus Addicks
    • Franz-Josef Klinz
  • View Affiliations / Copyright

    Affiliations: Department I of Anatomy, University of Cologne, D-50931 Cologne, Germany
  • Pages: 2001-2008
    |
    Published online on: January 27, 2015
       https://doi.org/10.3892/or.2015.3751
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Abstract

Glioblastoma (GB) is the most frequent human brain tumor and is associated with a poor prognosis. Multipolar mitosis and spindles have occasionally been observed in cultured glioblastoma cells and in glioblastoma tissues, but their mode of origin and relevance have remained unclear. In the present study, we investigated a novel GB cell line (SGB4) exhibiting mitotic aberrations and established a functional link between cytokinesis failure, centrosome amplification, multipolar mitosis and aneuploidy in glioblastoma. Long-term live cell imaging showed that >3% of mitotic SGB4 cells underwent multipolar mitosis (tripolar > tetrapolar > pentapolar). A significant amount of daugther cells generated by multipolar mitosis were viable and completed several rounds of mitosis. Pedigree analysis of mitotic events revealed that in many cases a bipolar mitosis with failed cytokinesis occurred prior to a multipolar mitosis. Additionally, we observed that SGB4 cells were also able to undergo a bipolar mitosis after failed cytokinesis. Colchicine-induced mitotic arrest and metaphase spreads demonstrated that SGB4 cells had a modal chromosome number of 58 ranging from 23 to 170. Approximately 82% of SGB4 cells were hyperdiploid (47-57 chromosomes) or hypotriploid (58-68 chromosomes). In conclusion, SGB4 cells passed through multipolar cell divisions and generated viable progeny by reductive mitoses. Our results identified cytokinesis failure occurring before and after multipolar or bipolar mitoses as important mechanisms to generate chromosomal heterogeneity in glioblastoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Telentschak S, Soliwoda M, Nohroudi K, Addicks K and Klinz F: Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells. Oncol Rep 33: 2001-2008, 2015.
APA
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., & Klinz, F. (2015). Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells. Oncology Reports, 33, 2001-2008. https://doi.org/10.3892/or.2015.3751
MLA
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., Klinz, F."Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells". Oncology Reports 33.4 (2015): 2001-2008.
Chicago
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., Klinz, F."Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells". Oncology Reports 33, no. 4 (2015): 2001-2008. https://doi.org/10.3892/or.2015.3751
Copy and paste a formatted citation
x
Spandidos Publications style
Telentschak S, Soliwoda M, Nohroudi K, Addicks K and Klinz F: Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells. Oncol Rep 33: 2001-2008, 2015.
APA
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., & Klinz, F. (2015). Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells. Oncology Reports, 33, 2001-2008. https://doi.org/10.3892/or.2015.3751
MLA
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., Klinz, F."Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells". Oncology Reports 33.4 (2015): 2001-2008.
Chicago
Telentschak, S., Soliwoda, M., Nohroudi, K., Addicks, K., Klinz, F."Cytokinesis failure and successful multipolar mitoses drive aneuploidy in glioblastoma cells". Oncology Reports 33, no. 4 (2015): 2001-2008. https://doi.org/10.3892/or.2015.3751
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