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Article

Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma

  • Authors:
    • Shuang Wang
    • Shisheng Li
    • Qinglai Tang
    • Shu Yang
    • Shuhui Wang
    • Jiajia Liu
    • Mi Yang
    • Xinming Yang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1807-1814
    |
    Published online on: February 5, 2015
       https://doi.org/10.3892/or.2015.3785
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Abstract

T-lymphoma invasion and metastasis‑inducing factor 1 (Tiam1) has been reported in various types of human cancer, which play important roles in facilitating the meta­stasis of malignant tumor. However, the investigation of Tiam1 in laryngeal squamous-cell carcinoma is extremely rare. The aim of the present study was to assess Tiam1 expression and examine its function in tumorigenesis and the metastasis of laryngeal squamous cell carcinoma (LSCC) in vitro. Tiam1 expression in 98 primary LSCC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' survival. To investigate the effects of Tiam1 on the progression of LSCC, Tiam1/C1199 plasmid was transfected into LSCC, and proliferation, apoptosis, migration and invasion of transfected cells were examined using MTT, flow cytometry, wound-healing and Transwell assay, respectively. The results showed that, Tiam1 was detected in all primary LSCC samples. Additionally, Tiam1 overexpression was closely correlated with tumor progression and patient survival. Tiam1 overexpression was statistically significant, and served as an independent predictor of prognosis for patients with LSCC. The upregulation of Tiam1 by Tiam1/C1199 plasmid had no effect on the prolife­ration of transfected cells, but decreased the apoptotic rate of transfected cells, while the ability of migration and invasion was increased. These results suggested that Tiam1 overexpression in LSCC is possibly involved in the promotion of migration and invasion, and is a promising therapeutic target in the prevention of the progression of LSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Li S, Tang Q, Yang S, Wang S, Liu J, Yang M and Yang X: Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma. Oncol Rep 33: 1807-1814, 2015.
APA
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J. ... Yang, X. (2015). Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma. Oncology Reports, 33, 1807-1814. https://doi.org/10.3892/or.2015.3785
MLA
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J., Yang, M., Yang, X."Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma". Oncology Reports 33.4 (2015): 1807-1814.
Chicago
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J., Yang, M., Yang, X."Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma". Oncology Reports 33, no. 4 (2015): 1807-1814. https://doi.org/10.3892/or.2015.3785
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Li S, Tang Q, Yang S, Wang S, Liu J, Yang M and Yang X: Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma. Oncol Rep 33: 1807-1814, 2015.
APA
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J. ... Yang, X. (2015). Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma. Oncology Reports, 33, 1807-1814. https://doi.org/10.3892/or.2015.3785
MLA
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J., Yang, M., Yang, X."Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma". Oncology Reports 33.4 (2015): 1807-1814.
Chicago
Wang, S., Li, S., Tang, Q., Yang, S., Wang, S., Liu, J., Yang, M., Yang, X."Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma". Oncology Reports 33, no. 4 (2015): 1807-1814. https://doi.org/10.3892/or.2015.3785
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