Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2015 Volume 33 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2015 Volume 33 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis

  • Authors:
    • Blendi Ura
    • Federica Scrimin
    • Fabrizio Zanconati
    • Giorgio Arrigoni
    • Lorenzo Monasta
    • Andrea Romano
    • Rubina Banco
    • Marina Zweyer
    • Daniela Milani
    • Giuseppe Ricci
  • View Affiliations / Copyright

    Affiliations: Institute for Maternal and Child Health - IRCCS ‘Burlo Garofolo’, Trieste, Italy, Department of Medical, Surgery and Health Sciences, University of Trieste, Trieste, Italy, Department of Biomedical Sciences, University of Padua, Padua, Italy, UCO Pathological Anatomy and Histology, Azienda Ospedaliera-Universitaria Ospedali Riuniti, Trieste, Italy, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
  • Pages: 2219-2226
    |
    Published online on: March 3, 2015
       https://doi.org/10.3892/or.2015.3827
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Uterine leiomyoma is the most common smooth benign neoplasm. In the present study, we analyzed the global interstitial fluid (IF) profile of leiomyoma vs. normal myometrium to identify protein dysregulation involved in leiomyoma pathogenesis. Two-dimensional gel electrophoresis and mass spectrometry were used to generate and compare the global interstitial fluid profiles of the leiomyoma and of the normal tissue. Two proteins were validated by immunohistochemistry. By comparing the interstitial fluid profile of the leiomyoma with that of the normal myometrium, the levels of seven proteins were found to be significantly different: four structural organization proteins (desmin, prelamin-A/C, transgelin and α-actinin-1), an inflammatory response (α1-antitrypsin), a response to oxidative stress (peroxiredoxin-2), and a folding protein (heat shock 70 kDa protein 1A/1B). Desmin, α1-antitrypsin and peroxiredoxin-2 were upregulated in the leiomyoma, whereas heat shock 70 kDa protein 1A/1B, α-actinin-1, prelamin-A/C and transgelin were downregulated. Desmin and α1-antitrypsin were further validated by immunohistochemistry. By identifying proteins with altered expression levels compared to the myometrium from several pathways of the leiomyoma pathogenesis, we found the leiomyoma interstitial fluid to have a characteristic proteomic profile. A better appreciation of the pathophysiology of the disease can be useful in the development of conservative treatments that serve as viable alternatives to hysterectomy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jacobson GF, Shaber RE, Armstrong MA and Hung YY: Hysterectomy rates for benign indications. Obstet Gynecol. 107:1278–1283. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Lepine LA, Hillis SD, Marchbanks PA, Koonin LM, Morrow B, Kieke BA and Wilcox LS: Hysterectomy surveillance - United States, 1980–1993. MMWR CDC Surveill Summ. 46:1–15. 1997.PubMed/NCBI

3 

Bernstein SJ, McGlynn EA, Siu AL, Roth CP, Sherwood MJ, Keesey JW, Kosecoff J, Hicks NR and Brook RH: The appropriateness of hysterectomy. A comparison of care in seven health plans Health Maintenance Organization Quality of Care Consortium. JAMA. 269:2398–2402. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Okolo S: Incidence, aetiology and epidemiology of uterine fibroids. Best Pract Res Clin Obstet Gynaecol. 22:571–588. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Chegini N, Ma C, Tang XM and Williams RS: Effects of GnRH analogues, ‘add-back’ steroid therapy, antiestrogen and anti-progestins on leiomyoma and myometrial smooth muscle cell growth and transforming growth factor-beta expression. Mol Hum Reprod. 8:1071–1078. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Wei T, Geiser AG, Qian HR, Su C, Helvering LM, Kulkarini NH, Shou J, N’Cho M, Bryant HU and Onyia JE: DNA microarray data integration by ortholog gene analysis reveals potential molecular mechanisms of estrogen-dependent growth of human uterine fibroids. BMC Womens Health. 7:52007. View Article : Google Scholar : PubMed/NCBI

7 

Kim JJ and Sefton EC: The role of progesterone signaling in the pathogenesis of uterine leiomyoma. Mol Cell Endocrinol. 358:223–231. 2012. View Article : Google Scholar

8 

Maruo T, Ohara N, Matsuo H, Xu Q, Chen W, Sitruk-Ware R and Johansson ED: Effects of levonorgestrel-releasing IUS and progesterone receptor modulator PRM CDB-2914 on uterine leiomyomas. Contraception. 75:S99–103. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Ciarmela P, Bloise E, Gray PC, Carrarelli P, Islam MS, De Pascalis F, Severi FM, Vale W, Castellucci M and Petraglia F: Activin-A and myostatin response and steroid regulation in human myometrium: disruption of their signalling in uterine fibroid. J Clin Endocrinol Metab. 96:755–765. 2011. View Article : Google Scholar :

10 

Ciavattini A, Di Giuseppe J, Stortoni P, Montik N, Giannubilo SR, Litta P, Islam MS, Tranquilli AL, Reis FM and Ciarmela P: Uterine fibroids: pathogenesis and interactions with endometrium and endomyometrial junction. Obstet Gynecol Int. 2013:1731842013.PubMed/NCBI

11 

Norian JM, Owen CM, Taboas J, Korecki C, Tuan R, Malik M, Catherino WH and Segars JH: Characterization of tissue biomechanics and mechanical signaling in uterine leiomyoma. Matrix Biol. 31:57–65. 2012. View Article : Google Scholar

12 

Alenghat FJ and Ingber DE: Mechanotransduction: all signals point to cytoskeleton, matrix, and integrins. Sci STKE. 2002:pe62002.PubMed/NCBI

13 

Paszek MJ, Zahir N, Johnson KR, Lakins JN, Rozenberg GI, Gefen A, Reinhart-King CA, Margulies SS, Dembo M, Boettiger D, Hammer DA and Weaver VM: Tensional homeostasis and the malignant phenotype. Cancer Cell. 8:241–254. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Butcher DT, Alliston T and Weaver VM: A tense situation: forcing tumour progression. Nat Rev Cancer. 9:108–122. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Baronzio G, Schwartz L, Kiselevsky M, Guais A, Sanders E, Milanesi G, Baronzio M and Freitas I: Tumor interstitial fluid as modulator of cancer inflammation, thrombosis, immunity and angiogenesis. Anticancer Res. 32:405–414. 2012.PubMed/NCBI

16 

Gromov P, Gromova I, Bunkenborg J, Cabezon T, Moreira JM, Timmermans-Wielenga V, Roepstorff P, Rank F and Celis JE: Up-regulated proteins in the fluid bathing the tumour cell micro-environment as potential serological markers for early detection of cancer of the breast. Mol Oncol. 4:65–89. 2010. View Article : Google Scholar

17 

Lv J, Zhu X, Dong K, Lin Y, Hu Y and Zhu C: Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics. Fertil Steril. 90:1892–1898. 2008. View Article : Google Scholar

18 

Lin CP, Chen YW, Liu WH, Chou HC, Chang YP, Lin ST, Li JM, Jian SF, Lee YR and Chan HL: Proteomic identification of plasma biomarkers in uterine leiomyoma. Mol Biosyst. 8:1136–1145. 2012. View Article : Google Scholar

19 

Bertacco E, Millioni R, Arrigoni G, Faggin E, Iop L, Puato M, Pinna LA, Tessari P, Pauletto P and Rattazzi M: Proteomic analysis of clonal interstitial aortic valve cells acquiring a pro-calcific profile. J Proteome Res. 9:5913–5921. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Gugliotta L, Bagnara GP, Catani L, Gaggioli L, Guarini A, Zauli G, Belmonte MM, Lauria F, Macchi S and Tura S: In vivo and in vitro inhibitory effect of alpha-interferon on mega-karyocyte colony growth in essential thrombocythaemia. Br J Haematol. 71:177–181. 1989. View Article : Google Scholar : PubMed/NCBI

21 

Secchiero P, Sun D, De Vico AL, Crowley RW, Reitz MS Jr, Zauli G, Lusso P and Gallo RC: Role of the extracellular domain of human herpesvirus 7 glycoprotein B in virus binding to cell surface heparan sulfate proteoglycans. J Virol. 71:4571–4580. 1997.PubMed/NCBI

22 

Zauli G, Secchiero P, Rodella L, Gibellini D, Mirandola P, Mazzoni M, Milani D, Dowd DR, Capitani S and Vitale M: HIV-1 Tat-mediated inhibition of the tyrosine hydroxylase gene expression in dopaminergic neuronal cells. J Biol Chem. 275:4159–4165. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Pavlou MP and Diamandis EP: The cancer cell secretome: a good source for discovering biomarkers? J Proteomics. 73:1896–1906. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Suehara Y, Kondo T, Fujii K, Hasegawa T, Kawai A, Seki K, Beppu Y, Nishimura T, Kurosawa H and Hirohashi S: Proteomic signatures corresponding to histological classification and grading of soft-tissue sarcomas. Proteomics. 6:4402–4409. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Bär H, Strelkov SV, Sjöberg G, Aebi U and Herrmann H: The biology of desmin filaments: how do mutations affect their structure, assembly, and organisation? J Struct Biol. 148:137–152. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Peddada SD, Laughlin SK, Miner K, Guyon JP, Haneke K, Vahdat HL, Semelka RC, Kowalik A, Armao D, Davis B and Baird DD: Growth of uterine leiomyomata among premenopausal black and white women. Proc Natl Acad Sci USA. 105:19887–19892. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Wettschureck N and Offermanns S: Rho/Rho-kinase mediated signaling in physiology and pathophysiology. J Mol Med (Berl). 80:629–638. 2002. View Article : Google Scholar

28 

Ren XD, Kiosses WB and Schwartz MA: Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 18:578–585. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Kubota D, Mukaihara K, Yoshida A, Tsuda H, Kawai A and Kondo T: Proteomics study of open biopsy samples identifies peroxiredoxin 2 as a predictive biomarker of response to induction chemotherapy in osteosarcoma. J Proteomics. 91:393–404. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Bae SM, Kim YW, Lee JM, Namkoong SE, Kim CK and Ahn WS: Expression profiling of the cellular processes in uterine leiomyomas: omic approaches and IGF-2 association with leiomyosarcomas. Cancer Res Treat. 36:31–42. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Mesquita FS, Dyer SN, Heinrich DA, Bulun SE, Marsh EE and Nowak RA: Reactive oxygen species mediate mitogenic growth factor signaling pathways in human leiomyoma smooth muscle cells. Biol Reprod. 82:341–351. 2010. View Article : Google Scholar :

32 

Secchiero P and Zauli G: Tumor-necrosis-factor-related apoptosis-inducing ligand and the regulation of hematopoiesis. Curr Opin Hematol. 15:42–48. 2008. View Article : Google Scholar

33 

Zauli G, Melloni E, Capitani S and Secchiero P: Role of full-length osteoprotegerin in tumor cell biology. Cell Mol Life Sci. 66:841–851. 2009. View Article : Google Scholar

34 

Karlisch C, Harati K, Chromik AM, Bulut D, Klein-Hitpass L, Goertz O, Hirsch T, Lehnhardt M, Uhl W and Daigeler A: Effects of TRAIL and taurolidine on apoptosis and proliferation in human rhabdomyosarcoma, leiomyosarcoma and epithelioid cell sarcoma. Int J Oncol. 42:945–956. 2013.PubMed/NCBI

35 

De Vos WH, Houben F, Hoebe RA, Hennekam R, van Engelen B, Manders EM, Ramaekers FC, Broers JL and Van Oostveldt P: Increased plasticity of the nuclear envelope and hypermobility of telomeres due to the loss of A-type lamins. Biochim Biophys Acta. 1800:448–458. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Ragnauth CD, Warren DT, Liu Y, Mcnair R, Tajsic T, Figg N, Shroff R, Skepper J and Shanahan CM: Prelamin A acts to accelerate smooth muscle cell senescence and is a novel biomarker of human vascular aging. Circulation. 121:2200–2210. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Kong L, Schäfer G, Bu H, Zhang Y, Zhang Y and Klocker H: Lamin A/C protein is overexpressed in tissue-invading prostate cancer and promotes prostate cancer cell growth, migration and invasion through the PI3K/AKT/PTEN pathway. Carcinogenesis. 33:751–759. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Li Q, Shi R, Wang Y and Niu X: TAGLN suppresses proliferation and invasion, and induces apoptosis of colorectal carcinoma cells. Tumour Biol. 34:505–513. 2013. View Article : Google Scholar

39 

Robin YM1, Penel N, Pérot G, Neuville A, Vélasco V, Ranchère-Vince D, Terrier P and Coindre JM: Transgelin is a novel marker of smooth muscle differentiation that improves diagnostic accuracy of leiomyosarcomas: a comparative immunohistochemical reappraisal of myogenic markers in 900 soft tissue tumors. Mod Pathol. 26:502–510. 2013. View Article : Google Scholar

40 

Yang C and Glass WF II: Expression of alpha-actinin-1 in human glomerular mesangial cells in vivo and in vitro. Exp Biol Med (Maywood). 233:689–693. 2008. View Article : Google Scholar

41 

Otey CA and Carpen O: Alpha-actinin revisited: a fresh look at an old player. Cell Motil Cytoskeleton. 58:104–111. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ura B, Scrimin F, Zanconati F, Arrigoni G, Monasta L, Romano A, Banco R, Zweyer M, Milani D, Ricci G, Ricci G, et al: Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis. Oncol Rep 33: 2219-2226, 2015.
APA
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A. ... Ricci, G. (2015). Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis. Oncology Reports, 33, 2219-2226. https://doi.org/10.3892/or.2015.3827
MLA
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A., Banco, R., Zweyer, M., Milani, D., Ricci, G."Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis". Oncology Reports 33.5 (2015): 2219-2226.
Chicago
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A., Banco, R., Zweyer, M., Milani, D., Ricci, G."Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis". Oncology Reports 33, no. 5 (2015): 2219-2226. https://doi.org/10.3892/or.2015.3827
Copy and paste a formatted citation
x
Spandidos Publications style
Ura B, Scrimin F, Zanconati F, Arrigoni G, Monasta L, Romano A, Banco R, Zweyer M, Milani D, Ricci G, Ricci G, et al: Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis. Oncol Rep 33: 2219-2226, 2015.
APA
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A. ... Ricci, G. (2015). Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis. Oncology Reports, 33, 2219-2226. https://doi.org/10.3892/or.2015.3827
MLA
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A., Banco, R., Zweyer, M., Milani, D., Ricci, G."Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis". Oncology Reports 33.5 (2015): 2219-2226.
Chicago
Ura, B., Scrimin, F., Zanconati, F., Arrigoni, G., Monasta, L., Romano, A., Banco, R., Zweyer, M., Milani, D., Ricci, G."Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis". Oncology Reports 33, no. 5 (2015): 2219-2226. https://doi.org/10.3892/or.2015.3827
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team