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Article

miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer

  • Authors:
    • Yu Zhang
    • Xinying Wang
    • Zhongqiu Wang
    • Hui Tang
    • Hong Fan
    • Qiang Guo
  • View Affiliations / Copyright

    Affiliations: Yunnan Provincial Institute of Digestive Medicine, Department of Gastroenterology, The First People's Hospital of Yunnan Province, Kunming, P.R. China, Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China
  • Pages: 2592-2598
    |
    Published online on: March 4, 2015
       https://doi.org/10.3892/or.2015.3833
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Abstract

Forkhead box F2 transcription factor (FoxF2) has been described to promote organ development, extracellular matrix (ECM) synthesis and epithelial-mesenchymal interaction. Although recent studies reported decreased FoxF2 expression in several types of cancers, indicating its potential role in carcinogenesis, the mechanistic role of FoxF2 is yet to be explored. MicroRNAs (miRNAs) are strongly implicated in carcinogenesis. The oncogenetic properties of miR-182 have been described in multiple cancers. In the present study, we aimed to investigate the role of miR-182 in colorectal cancer (CRC) and identify the regulation of FoxF2 by miR-182. Bioinformatic analyses on gene expression profiling datasets showed decreased FoxF2 expression in colorectal adenomas, primary tumors compared to normal colon epithelial and a negative association between FoxF2 and β-catenin expression. Restoration of FoxF2 in CRC cells suppressed β-catenin expression and simultaneously inhibited cell growth and invasion. Furthermore, we observed that miR-182 was aberrantly upregulated in CRC. Knockdown of miR-182 in CRC cells impeded cell growth and invasion. The direct binding of miR-182 to the 3' untranslated region (3'UTR) of FoxF2 mRNA was confirmed using a luciferase reporter gene assay. Importantly, elevated FoxF2 expression was observed in miR-182-knockdown cells with a simultaneous reduction in β-catenin. In conclusion, the present study describes a potential mechanism underlying an miR-182/FoxF2 link contributing to CRC development. miR‑182-induced downregulation of FoxF2 partly accounts for increased activity of β-catenin signaling. Inhibition of miR-182 represents a potential strategy against CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Wang X, Wang Z, Tang H, Fan H and Guo Q: miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer. Oncol Rep 33: 2592-2598, 2015.
APA
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., & Guo, Q. (2015). miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer. Oncology Reports, 33, 2592-2598. https://doi.org/10.3892/or.2015.3833
MLA
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., Guo, Q."miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer". Oncology Reports 33.5 (2015): 2592-2598.
Chicago
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., Guo, Q."miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer". Oncology Reports 33, no. 5 (2015): 2592-2598. https://doi.org/10.3892/or.2015.3833
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Wang X, Wang Z, Tang H, Fan H and Guo Q: miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer. Oncol Rep 33: 2592-2598, 2015.
APA
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., & Guo, Q. (2015). miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer. Oncology Reports, 33, 2592-2598. https://doi.org/10.3892/or.2015.3833
MLA
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., Guo, Q."miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer". Oncology Reports 33.5 (2015): 2592-2598.
Chicago
Zhang, Y., Wang, X., Wang, Z., Tang, H., Fan, H., Guo, Q."miR-182 promotes cell growth and invasion by targeting forkhead box F2 transcription factor in colorectal cancer". Oncology Reports 33, no. 5 (2015): 2592-2598. https://doi.org/10.3892/or.2015.3833
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