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Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients

  • Authors:
    • Yoshihiko Shibayama
    • Takeshi Kondo
    • Hiroki Ohya
    • Shin-Ichi Fujisawa
    • Takanori Teshima
    • Ken Iseki
  • View Affiliations / Copyright

    Affiliations: Education Research Center for Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan, Department of Hematology, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan, Laboratory of Clinical Pharmaceutics and Therapeutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan, Department of Clinical Laboratory, Hokkaido University Hospital, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan
    Copyright: © Shibayama et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2176-2182
    |
    Published online on: March 6, 2015
       https://doi.org/10.3892/or.2015.3839
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Abstract

MicroRNAs (miRs) have been shown to ne­gatively regulate gene expression by binding to mRNAs, and they play an important role in various physiological processes and ma­lignancies. A previous study identified mature miR‑126-3p as an onco-microRNA that is generated from the pre‑microRNA, miR-126. Although miR-126 also generates mature miR‑126-5p, its function is less clear. In the present study, the relationship between miR-126-5p/3p expression levels and overall survival in 109 patients with acute myeloid leukemia (AML) who received intensive therapy were evaluated. Higher expression levels above the median value of miR-126-5p/3p were correlated with a poorer overall survival. The hazard ratio and 95% confidence intervals (95% CI) for the higher expression group relative to the lower expression group of miR-126-5p/3p were 2.098 (95% CI: 1.075-4.228) and 1.958 (95% CI: 1.001-3.927), respectively. An interaction was not observed between the hazard ratios of miR-126-5p and miR‑126-3p (p=0.73). Transfection of the mimic miR-126-5p into the AML cell line, KG-1, resulted in a decrease in the sensitivity to cytarabin and the expression level of Klotho mRNA as well as the elevation in the phosphorylation of Akt. The results of the present study demonstrated that higher expression levels of miR-126-5p/3p in patients with AML resulted in a poorer prognosis. Furthermore, miR-126-5p elevated the phosphorylation of Akt.
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Copy and paste a formatted citation
Spandidos Publications style
Shibayama Y, Kondo T, Ohya H, Fujisawa S, Teshima T and Iseki K: Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncol Rep 33: 2176-2182, 2015.
APA
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., & Iseki, K. (2015). Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncology Reports, 33, 2176-2182. https://doi.org/10.3892/or.2015.3839
MLA
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., Iseki, K."Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients". Oncology Reports 33.5 (2015): 2176-2182.
Chicago
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., Iseki, K."Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients". Oncology Reports 33, no. 5 (2015): 2176-2182. https://doi.org/10.3892/or.2015.3839
Copy and paste a formatted citation
x
Spandidos Publications style
Shibayama Y, Kondo T, Ohya H, Fujisawa S, Teshima T and Iseki K: Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncol Rep 33: 2176-2182, 2015.
APA
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., & Iseki, K. (2015). Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncology Reports, 33, 2176-2182. https://doi.org/10.3892/or.2015.3839
MLA
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., Iseki, K."Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients". Oncology Reports 33.5 (2015): 2176-2182.
Chicago
Shibayama, Y., Kondo, T., Ohya, H., Fujisawa, S., Teshima, T., Iseki, K."Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients". Oncology Reports 33, no. 5 (2015): 2176-2182. https://doi.org/10.3892/or.2015.3839
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