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Article

Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma

  • Authors:
    • Mei Liu
    • Armando Varela-Ramirez
    • Jitian Li
    • Liping Dai
    • Renato J. Aguilera
    • Jian-Ying Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences and Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA
  • Pages: 2245-2252
    |
    Published online on: March 16, 2015
       https://doi.org/10.3892/or.2015.3854
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Abstract

Hepatocellular carcinoma (HCC) is a worldwide prevalent cancer with an exremely poor prognosis. Detection of serum α-fetoprotein (AFP) and liver imaging techniques are the conventional methods used clinically for the identification of this malignancy. However, these techniques are not reliable for early diagnosis, and particularly the sensitivity and specificity of AFP in HCC diagnosis are not optimal. Therefore, there is an urgent need for the development of more sensitive and specific methods that can improve AFP quantification in the early detection of HCC. In the present study, autoantibody responses to nucleophosmin (NPM1) in HCC patients were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting and indirect immunofluorescence. Immunohistochemistry (IHC) with tissue array slides was also performed to analyze protein expression of NPM1 in HCC and control tissues. The prevalence of autoantibodies against NPM1 was 22.4% (17/76) in HCC, which was significantly higher than that in sera from patients with liver cirrhosis (LC), chronic hepatitis (CH) and systemic lupus erythematosus (SLE) (P<0.01). The average titer of autoantibodies against NPM1 in HCC sera was higher compared to that in LC, CH, SLE and normal human sera (NHS) (P<0.01). In addition, anti-NMP1 autoantibodies were detected in sera from several HCC patients with serial bleeding samples. A stronger reactive band corresponding to NMP1 was visualized in the western blot analyses, utilizing sera from patients 3-6 months before the clinical diagnosis of HCC. Our data indicate that NPM1 and the anti-NPM1 system may have potential as an early-stage biomarker for HCC screening and diagnosis.
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View References

1 

Borer RA, Lehner CF, Eppenberger HM and Nigg EA: Major nucleolar proteins shuttle between nucleus and cytoplasm. Cell. 56:379–390. 1989. View Article : Google Scholar : PubMed/NCBI

2 

Wang D, Umekawa H and Olson MO: Expression and subcellular locations of two forms of nucleolar protein B23 in rat tissues and cells. Cell Mol Biol Res. 39:33–42. 1993.PubMed/NCBI

3 

Grisendi S, Bernardi R, Rossi M, Cheng K, Khandker L, Manova K and Pandolfi PP: Role of nucleophosmin in embryonic development and tumorigenesis. Nature. 437:147–153. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Wu MH and Yung BY: UV stimulation of nucleophosmin/B23 expression is an immediate early gene response induced by damaged DNA. J Biol Chem. 277:48234–48240. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Verheggen C, Almouzni G and Hernandez-Verdun D: The ribosomal RNA processing machinery is recruited to the nucleolar domain before RNA polymerase I during Xenopus laevis sdevelopment. J Cell Biol. 149:293–306. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Huang N, Negi S, Szebeni A and Olson MO: Protein NPM3 interacts with the multifunctional nucleolar protein B23/nucleophosmin and inhibits ribosome biogenesis. J Biol Chem. 280:5496–5502. 2005. View Article : Google Scholar

7 

Okuda M: The role of nucleophosmin in centrosome duplication. Oncogene. 21:6170–6174. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Okuda M, Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, et al: Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication. Cell. 103:127–140. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Okuwaki M, Matsumoto K, Tsujimoto M and Nagata K: Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone. FEBS Lett. 506:272–276. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Lindström MS and Zhang Y: Ribosomal protein S9 is a novel B23/NPM-binding protein required for normal cell proliferation. J Biol Chem. 283:15568–15576. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Maggi LB Jr, Kuchenruether M, Dadey DY, Schwope RM, Grisendi S, Townsend RR, Pandolfi PP and Weber JD: Nucleophosmin serves as a rate-limiting nuclear export chaperone for the mammalian ribosome. Mol Cell Biol. 28:7050–7065. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Avitabile D, Bailey B, Cottage CT, Sundararaman B, Joyo A, McGregor M, Gude N, et al: Nucleolar stress is an early response to myocardial damage involving nucleolar proteins nucleostemin and nucleophosmin. Proc Natl Acad Sci USA. 108:6145–6150. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Khandelwal N, Simpson J, Taylor G, Rafique S, Whitehouse A, Hiscox J and Stark LA: Nucleolar NF-κB/RelA mediates apoptosis by causing cytoplasmic relocalization of nucleophosmin. Cell Death Differ. 18:1889–1903. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Li J, Zhang X, Sejas DP, Bagby GC and Pang Q: Hypoxia-induced nucleophosmin protects cell death through inhibition of p53. J Biol Chem. 279:41275–41279. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Colombo E, Alcalay M and Pelicci PG: Nucleophosmin and its complex network: a possible therapeutic target in hematological diseases. Oncogene. 30:2595–2609. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Colombo E, Marine JC, Danovi D, Falini B and Pelicci PG: Nucleophosmin regulates the stability and transcriptional activity of p53. Nat Cell Biol. 4:529–533. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Kurki S, Peltonen K, Latonen L, Kiviharju TM, Ojala PM, Meek D and Laiho M: Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation. Cancer Cell. 5:465–475. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Maiguel DA, Jones L, Chakravarty D, Yang C and Carrier F: Nucleophosmin sets a threshold for p53 response to UV radiation. Mol Cell Biol. 24:3703–3711. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Bertwistle D, Sugimoto M and Sherr CJ: Physical and functional interactions of the Arf tumor suppressor protein with nucleophosmin/B23. Mol Cell Biol. 24:985–996. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Falini B, Nicoletti I, Martelli MF and Mecucci C: Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 109:874–885. 2007. View Article : Google Scholar

21 

Meani N and Alcalay M: Role of nucleophosmin in acute myeloid leukemia. Expert Rev Anticancer Ther. 9:1283–1294. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Grisendi S, Mecucci C, Falini B and Pandolfi PP: Nucleophosmin and cancer. Nat Rev Cancer. 6:493–505. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Liu X, Liu D, Qian D, Dai J, An Y, Jiang S, Stanley B, et al: Nucleophosmin (NPM1/B23) interacts with activating transcription factor 5 (ATF5) protein and promotes proteasome- and caspase-dependent ATF5 degradation in hepatocellular carcinoma cells. J Biol Chem. 287:19599–19609. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Johnson PJ, Leung N, Cheng P, Welby C, Leung WT, Lau WY, Yu S and Ho S: ‘Hepatoma-specific’ alphafetoprotein may permit preclinical diagnosis of malignant change in patients with chronic liver disease. Br J Cancer. 75:236–240. 1997. View Article : Google Scholar

25 

Zhang JY, Casiano CA, Peng XX, Koziol JA, Chan EK and Tan EM: Enhancement of antibody detection in cancer using panel of recombinant tumor-associated antigens. Cancer Epidemiol Biomarkers Prev. 12:136–143. 2003.PubMed/NCBI

26 

Zhang JY, Megliorino R, Peng XX, Tan EM, Chen Y and Chan EK: Antibody detection using tumor-associated antigen mini-array in diagnosing human hepatocellular carcinoma. J Hepatol. 46:107–114. 2007. View Article : Google Scholar

27 

Looi KS, Nakayasu ES, Diaz RA, Tan EM, Almeida IC and Zhang JY: Using proteomic approach to identify tumor-associated antigens as markers in hepatocellular carcinoma. J Proteome Res. 7:4004–4012. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Zhang J, Wang K, Zhang J, Liu SS, Dai L and Zhang JY: Using proteomic approach to identify tumor-associated proteins as biomarkers in human esophageal squamous cell carcinoma. J Proteome Res. 10:2863–2872. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Ersvaer E, Zhang JY, McCormack E, Olsnes A, Anensen N, Tan EM, Gjertsen BT and Bruserud O: Cyclin B1 is commonly expressed in the cytoplasm of primary human acute myelogenous leukemia cells and serves as a leukemia-associated antigen associated with autoantibody response in a subset of patients. Eur J Haematol. 79:210–225. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Looi K, Megliorino R, Shi FD, Peng XX, Chen Y and Zhang JY: Humoral immune response to p16, a cyclin-dependent kinase inhibitor in human malignancies. Oncol Rep. 16:1105–1110. 2006.PubMed/NCBI

31 

Liu W, Wang P, Li Z, Xu W, Dai L, Wang K and Zhang J: Evaluation of tumour-associated antigen (TAA) miniarray in immunodiagnosis of colon cancer. Scand J Immunol. 69:57–63. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Vogelstein B and Kinzler W: The multistep nature of cancer. Trends Genet. 9:138–141. 1993. View Article : Google Scholar : PubMed/NCBI

33 

Hanahan D and Weinberg RA: Hallmarks of cancer: the next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Chen Y, Zhou Y, Qiu S, Wang K, Liu S, Peng XX, Li J, et al: Autoantibodies to tumor-associated antigens combined with abnormal alpha-fetoprotein enhance immunodiagnosis of hepatocellular carcinoma. Cancer Lett. 289:32–39. 2010. View Article : Google Scholar :

35 

Zhang JY: Mini-array of multiple tumor-associated antigens to enhance autoantibody detection for immunodiagnosis of hepatocellular carcinoma. Autoimmun Rev. 6:143–148. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Himoto T, Kuriyama S, Zhang JY, Chan EK, Nishioka M and Tan EM: Significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins in patients with hepatocellular carcinoma. Int J Oncol. 26:311–317. 2005.PubMed/NCBI

37 

Zhang JY and Tan EM: Autoantibodies to tumor-associated antigens as diagnostic biomarkers in hepatocellular carcinoma and other solid tumors. Expert Rev Mol Diagn. 10:321–328. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Chen W, Rassidakis GZ and Medeiros LJ: Nucleophosmin gene mutations in acute myeloid leukemia. Arch Pathol Lab Med. 130:1687–1692. 2006.PubMed/NCBI

39 

Chou SH, Ko BS, Chiou JS, Hsu YC, Tsai MH, Chiu YC, Yu IS, et al: A knock-in Npm1 mutation in mice results in myeloproliferation and implies a perturbation in hematopoietic microenvironment. PLoS One. 7:e497692012. View Article : Google Scholar : PubMed/NCBI

40 

Qin FX, Shao HY, Chen XC, Tan S, Zhang HJ, Miao ZY, Wang L, et al: Knockdown of NPM1 by RNA interference inhibits cells proliferation and induces apoptosis in leukemic cell line. Int J Med Sci. 8:287–294. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Tsui KH, Juang HH, Lee TH, Chang PL, Chen CL and Yung BY: Association of nucleophosmin/B23 with bladder cancer recurrence based on immunohistochemical assessment in clinical samples. Acta Pharmacol Sin. 29:364–370. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Montazeri A, Vahdaninia M, Harirchi I, et al: Breast cancer in Iran: need for greater women awareness of warning signs and effective screening methods. Asia Pac Fam Med. 7(6)2008. View Article : Google Scholar : PubMed/NCBI

43 

Pianta A, Puppin C, Passon N, Franzoni A, Romanello M, Tell G, Di Loreto C, et al: Nucleophosmin delocalization in thyroid tumour cells. Endocr Pathol. 22:18–23. 2011. View Article : Google Scholar : PubMed/NCBI

44 

Liu Y, Zhang F, Zhang XF, Qi LS, Yang L, Guo H and Zhang N: Expression of nucleophosmin/NPM1 correlates with migration and invasiveness of colon cancer cells. J Biomed Sci. 19(53)2012. View Article : Google Scholar

45 

Karhemo PR, Rivinoja A, Lundin J, Hyvönen M, Chernenko A, Lammi J, Sihto H, et al: An extensive tumor array analysis supports tumor suppressive role for nucleophosmin in breast cancer. Am J Pathol. 179:1004–1014. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Tsui KH, Cheng AJ, Chang Pe, Pan TL and Yung BY: Association of nucleophosmin/B23 mRNA expression with clinical outcome in patients with bladder carcinoma. Urology. 64:839–844. 2004. View Article : Google Scholar : PubMed/NCBI

47 

Skaar TC, Prasad SC, Sharareh S, Lippman ME, Brünner N and Clarke R: Two-dimensional gel electrophoresis analyses identify nucleophosmin as an estrogen regulated protein associated with acquired estrogen-independence in human breast cancer cells. J Steroid Biochem Mol Biol. 67:391–402. 1998. View Article : Google Scholar

48 

Yun JP, Miao J, Chen GG, Tian QH, Zhang CQ, Xiang J, Fu J and Lai PB: Increased expression of nucleophosmin/B23 in hepatocellular carcinoma and correlation with clinicopathological parameters. Br J Cancer. 96:477–484. 2007. View Article : Google Scholar : PubMed/NCBI

49 

Falini B, Mecucci C, Tiacci E, Alcalay M, Rosati R, Pasqualucci L, La Starza R, et al: ; GIMEMA Acute Leukemia Working Party: Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. N Engl J Med. 352:254–266. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu M, Varela-Ramirez A, Li J, Dai L, Aguilera RJ and Zhang J: Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma. Oncol Rep 33: 2245-2252, 2015.
APA
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R.J., & Zhang, J. (2015). Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma. Oncology Reports, 33, 2245-2252. https://doi.org/10.3892/or.2015.3854
MLA
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R. J., Zhang, J."Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma". Oncology Reports 33.5 (2015): 2245-2252.
Chicago
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R. J., Zhang, J."Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma". Oncology Reports 33, no. 5 (2015): 2245-2252. https://doi.org/10.3892/or.2015.3854
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Varela-Ramirez A, Li J, Dai L, Aguilera RJ and Zhang J: Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma. Oncol Rep 33: 2245-2252, 2015.
APA
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R.J., & Zhang, J. (2015). Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma. Oncology Reports, 33, 2245-2252. https://doi.org/10.3892/or.2015.3854
MLA
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R. J., Zhang, J."Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma". Oncology Reports 33.5 (2015): 2245-2252.
Chicago
Liu, M., Varela-Ramirez, A., Li, J., Dai, L., Aguilera, R. J., Zhang, J."Humoral autoimmune response to nucleophosmin in the immunodiagnosis of hepatocellular carcinoma". Oncology Reports 33, no. 5 (2015): 2245-2252. https://doi.org/10.3892/or.2015.3854
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