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Article

Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β

  • Authors:
    • Xiao-Jing Wang
    • You-Hua Xu
    • Gui-Cun Yang
    • Hong-Xia Chen
    • Ping Zhang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Developmental Diseases in Childhood, Chongqing, P.R. China
  • Pages: 2368-2374
    |
    Published online on: March 18, 2015
       https://doi.org/10.3892/or.2015.3860
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Abstract

Tetramethylpyrazine (TMP) has been proven to be an anticancer agent in many studies. However, its effectiveness in acute lymphoblastic leukemia (ALL) and its molecular mechanisms are still unclear. The present study aimed to evaluate the effect of TMP against Jurkat and SUP-B15 ALL cell lines and to investigate the possible detailed mechanism of action of TMP. A Cell Counting Kit-8 (CCK-8) assay was employed to examine the proliferation of Jurkat and SUP-B15 cells. Flow cytometric analysis was conducted to detect the cell cycle distribution and apoptotic rate. The expression of total glycogen synthase kinase-3β (GSK-3β), cox-2, survivin, bcl-2 and p27 RNA and protein levels was detected by quantitative real-time PCR and western blot assay, respectively. Additionally, western blot analysis was used to determine the whole-cell and nuclear protein levels of GSK-3β downstream transcription factors, NF-κB (p65) and c-myc. TMP inhibited the proliferation of Jurkat and SUP-B15 cells in a dose- and time-dependent manner, with IC50 values of 120 and 200 µg/ml, respectively at 48 h. TMP induced the apoptosis of Jurkat and SUP-B15 cells and synergistically blocked cell cycle progression at the G0/G1 phase. Cells treated with TMP exhibited significantly attenuated GSK-3β, NF-κB (p65) and c-myc expression, followed by downregulation of bcl-2, cox-2 and survivin and an upregulation of p27. The results showed that TMP induced apoptosis and caused cell cycle arrest in Jurkat and SUP-B15 cells through the downregulation of GSK-3β, which may have further prevented the induced translocation of NF-κB and c-myc from the cytoplasm to the nucleus.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Xu Y, Yang G, Chen H and Zhang P: Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β. Oncol Rep 33: 2368-2374, 2015.
APA
Wang, X., Xu, Y., Yang, G., Chen, H., & Zhang, P. (2015). Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β. Oncology Reports, 33, 2368-2374. https://doi.org/10.3892/or.2015.3860
MLA
Wang, X., Xu, Y., Yang, G., Chen, H., Zhang, P."Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β". Oncology Reports 33.5 (2015): 2368-2374.
Chicago
Wang, X., Xu, Y., Yang, G., Chen, H., Zhang, P."Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β". Oncology Reports 33, no. 5 (2015): 2368-2374. https://doi.org/10.3892/or.2015.3860
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Xu Y, Yang G, Chen H and Zhang P: Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β. Oncol Rep 33: 2368-2374, 2015.
APA
Wang, X., Xu, Y., Yang, G., Chen, H., & Zhang, P. (2015). Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β. Oncology Reports, 33, 2368-2374. https://doi.org/10.3892/or.2015.3860
MLA
Wang, X., Xu, Y., Yang, G., Chen, H., Zhang, P."Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β". Oncology Reports 33.5 (2015): 2368-2374.
Chicago
Wang, X., Xu, Y., Yang, G., Chen, H., Zhang, P."Tetramethylpyrazine inhibits the proliferation of acute lymphocytic leukemia cell lines via decrease in GSK-3β". Oncology Reports 33, no. 5 (2015): 2368-2374. https://doi.org/10.3892/or.2015.3860
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