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Article Open Access

Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells

  • Authors:
    • Aiwei Yao-Borengasser
    • Behjatolah Monzavi-Karbassi
    • Rebecca A. Hedges
    • Lora J. Rogers
    • Susan A. Kadlubar
    • Thomas Kieber-Emmons
  • View Affiliations / Copyright

    Affiliations: Division of Medical Genetics, College of Medicine, and Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA, Department of Pathology, College of Medicine, and Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Copyright: © Yao-Borengasser et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2689-2694
    |
    Published online on: March 30, 2015
       https://doi.org/10.3892/or.2015.3880
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Abstract

The development of breast cancer is linked to the loss of estrogen receptor (ER) during the course of tumor progression, resulting in loss of responsiveness to hormonal treatment. The mechanisms underlying dynamic ERα gene expression change in breast cancer remain unclear. A range of physiological and biological changes, including increased adipose tissue hypoxia, accompanies obesity. Hypoxia in adipocytes can establish a pro-malignancy environment in breast tissues. Epidemiological studies have linked obesity with basal-like breast cancer risk and poor disease outcome, suggesting that obesity may affect the tumor phenotype by skewing the microenvironment toward support of more aggressive tumor phenotypes. In the present study, human SGBS adipocytes were co-cultured with ER-positive MCF7 cells for 24 h. After co-culture, HIF1α, TGF-β, and lectin-type oxidized LDL receptor 1 (LOX1) mRNA levels in the SGBS cells were increased. Expression levels of the epithelial-mesenchymal transition (EMT)-inducing transcription factors FOXC2 and TWIST1 were increased in the co-cultured MCF7 cells. In addition, the E-cadherin mRNA level was decreased, while the N-cadherin mRNA level was increased in the co-cultured MCF7 cells. ERα mRNA levels were significantly repressed in the co-cultured MCF7 cells. ERα gene expression in the MCF7 cells was decreased due to increased HIF1α in the SGBS cells. These results suggest that adipocytes can modify breast cancer cell ER gene expression through hypoxia and also can promote EMT processes in breast cancer cells, supporting an important role of obesity in aggressive breast cancer development.
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Copy and paste a formatted citation
Spandidos Publications style
Yao-Borengasser A, Monzavi-Karbassi B, Hedges RA, Rogers LJ, Kadlubar SA and Kieber-Emmons T: Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells. Oncol Rep 33: 2689-2694, 2015.
APA
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R.A., Rogers, L.J., Kadlubar, S.A., & Kieber-Emmons, T. (2015). Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells. Oncology Reports, 33, 2689-2694. https://doi.org/10.3892/or.2015.3880
MLA
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R. A., Rogers, L. J., Kadlubar, S. A., Kieber-Emmons, T."Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells". Oncology Reports 33.6 (2015): 2689-2694.
Chicago
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R. A., Rogers, L. J., Kadlubar, S. A., Kieber-Emmons, T."Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells". Oncology Reports 33, no. 6 (2015): 2689-2694. https://doi.org/10.3892/or.2015.3880
Copy and paste a formatted citation
x
Spandidos Publications style
Yao-Borengasser A, Monzavi-Karbassi B, Hedges RA, Rogers LJ, Kadlubar SA and Kieber-Emmons T: Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells. Oncol Rep 33: 2689-2694, 2015.
APA
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R.A., Rogers, L.J., Kadlubar, S.A., & Kieber-Emmons, T. (2015). Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells. Oncology Reports, 33, 2689-2694. https://doi.org/10.3892/or.2015.3880
MLA
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R. A., Rogers, L. J., Kadlubar, S. A., Kieber-Emmons, T."Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells". Oncology Reports 33.6 (2015): 2689-2694.
Chicago
Yao-Borengasser, A., Monzavi-Karbassi, B., Hedges, R. A., Rogers, L. J., Kadlubar, S. A., Kieber-Emmons, T."Adipocyte hypoxia promotes epithelial-mesenchymal transition-related gene expression and estrogen receptor-negative phenotype in breast cancer cells". Oncology Reports 33, no. 6 (2015): 2689-2694. https://doi.org/10.3892/or.2015.3880
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