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Article

miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein

  • Authors:
    • Fuyao Li
    • Huijun Chen
    • Yibo Huang
    • Qian Zhang
    • Jisen Xue
    • Zan Liu
    • Feiyun Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of General Surgery, Zhuzhou Kind Hospital, Zhuzhou, Hunan 412000, P.R. China
  • Pages: 3069-3074
    |
    Published online on: April 2, 2015
       https://doi.org/10.3892/or.2015.3894
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Abstract

Endometrial carcinoma (EC) is a common malignancy of the female genital tract with a poor prognosis. It has been reported that miR-34c is significantly reduced in EC, but research concerning its function in EC is rare. In the present study, the expression of miR-34c was upregulated in the EC cell line, HEC-1-B, by transfecting the cells with hsa-miR-34c-5p mimics. Then, after determining the transfection efficiency by RT-qPCR, we analyzed the effects of miR-34c on the HEC-1-B cells. We found that overexpression of miR-34c significantly inhibited cell proliferation, colony formation, migration and invasion and induced cell cycle arrest and apoptosis. Finally, western blot analysis demonstrated that the expression of E2F3 was reduced after upregulation of the expression of miR-34c in the HEC-1-B cells, and the effects of miR-34c are likely associated with the reduction in E2F3 protein. In conclusion, our study demonstrated that miR-34c plays a role of tumor suppressor in HEC-1-B cells, and E2F3 protein may be a target of miR-34c.
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Copy and paste a formatted citation
Spandidos Publications style
Li F, Chen H, Huang Y, Zhang Q, Xue J, Liu Z and Zheng F: miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein. Oncol Rep 33: 3069-3074, 2015.
APA
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., & Zheng, F. (2015). miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein. Oncology Reports, 33, 3069-3074. https://doi.org/10.3892/or.2015.3894
MLA
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., Zheng, F."miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein". Oncology Reports 33.6 (2015): 3069-3074.
Chicago
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., Zheng, F."miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein". Oncology Reports 33, no. 6 (2015): 3069-3074. https://doi.org/10.3892/or.2015.3894
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Chen H, Huang Y, Zhang Q, Xue J, Liu Z and Zheng F: miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein. Oncol Rep 33: 3069-3074, 2015.
APA
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., & Zheng, F. (2015). miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein. Oncology Reports, 33, 3069-3074. https://doi.org/10.3892/or.2015.3894
MLA
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., Zheng, F."miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein". Oncology Reports 33.6 (2015): 3069-3074.
Chicago
Li, F., Chen, H., Huang, Y., Zhang, Q., Xue, J., Liu, Z., Zheng, F."miR-34c plays a role of tumor suppressor in HEC‑1-B cells by targeting E2F3 protein". Oncology Reports 33, no. 6 (2015): 3069-3074. https://doi.org/10.3892/or.2015.3894
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