IL-33-induced JNK pathway activation confers gastric cancer chemotherapy resistance

  • Authors:
    • Xiao-Lei Ye
    • Ya-Rong Zhao
    • Guo-Bin Weng
    • Yi-Chen Chen
    • Xue-Ni Wei
    • Jing-Ping Shao
    • Hui Ji
  • View Affiliations

  • Published online on: April 3, 2015     https://doi.org/10.3892/or.2015.3898
  • Pages: 2746-2752
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Abstract

Inflammation is regarded as one of the major hallmarks of tumors, and has a very close relationship with gastric cancer. Interleukin-33 (IL-33), a new member of the IL-1 family, plays an important role in both inflammatory disease and tumors. The present study was designed to explore the effects of IL-33 on the proliferation, drug sensitivity, and the invasiveness of gastric cancer cells in vitro. IL-33 at concentrations lower than 100 pg/ml did not alter the inhibitory rate of gastric cancer cells. Moreover, IL-33 at these low concentrations protected against platinum-induced apoptosis in various gastric cancer cell lines, yet not in normal gastric epithelial cells. We also found that IL-33 increased the activation of the JNK pathway, and enhanced the expression of ST2. Furthermore, SP600125, a selective inhibitor of the JNK pathway, significantly blocked the protective effects of IL-33 in gastric cancer cells. In addition, Matrigel invasion assay showed that IL-33 markedly promoted gastric cancer cell invasion. In conclusion, the present study demonstrated that IL-33 protected against platinum-induced apoptosis and promoted cell invasion via activation of the JNK pathway in gastric cancer cells. In light of the prevalence of platinum-based chemotherapeutics in the treatment of gastric cancer, our results suggest that the level of IL-33 should be monitored during the treatment of gastric cancer, particularly when using platinum-based chemotherapeutics.
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June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ye X, Zhao Y, Weng G, Chen Y, Wei X, Shao J and Ji H: IL-33-induced JNK pathway activation confers gastric cancer chemotherapy resistance. Oncol Rep 33: 2746-2752, 2015
APA
Ye, X., Zhao, Y., Weng, G., Chen, Y., Wei, X., Shao, J., & Ji, H. (2015). IL-33-induced JNK pathway activation confers gastric cancer chemotherapy resistance. Oncology Reports, 33, 2746-2752. https://doi.org/10.3892/or.2015.3898
MLA
Ye, X., Zhao, Y., Weng, G., Chen, Y., Wei, X., Shao, J., Ji, H."IL-33-induced JNK pathway activation confers gastric cancer chemotherapy resistance". Oncology Reports 33.6 (2015): 2746-2752.
Chicago
Ye, X., Zhao, Y., Weng, G., Chen, Y., Wei, X., Shao, J., Ji, H."IL-33-induced JNK pathway activation confers gastric cancer chemotherapy resistance". Oncology Reports 33, no. 6 (2015): 2746-2752. https://doi.org/10.3892/or.2015.3898