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Article

Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6

  • Authors:
    • Chunhui Jin
    • Yingying Liu
    • Jianhong Zhu
    • Tingting Xia
    • Baonan Zhang
    • Yajun Fei
    • Bo Ma
    • Jianxin Ye
    • Weichang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Oncology, Wuxi Hospital of TCM, Wuxi, Jiangsu 214000, P.R. China, Department of Pathology, Wuxi Hospital of TCM, Wuxi, Jiangsu 214000, P.R. China, Department of Surgery, Huzhou Center Hospital, Huzhou, Zhejiang 313000, P.R. China
  • Pages: 2837-2844
    |
    Published online on: April 7, 2015
       https://doi.org/10.3892/or.2015.3901
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Abstract

The present study aimed to determine the effect of recombinant Salmonella (SL3261)-based CEACAM6 and 4-1BB ligand (4-1BBL) vaccine on the development of colorectal cancer in rats and the potential immune mechanisms involved. Attenuated Salmonella typhimurium (vaccine strain)‑carrying plasmids pIRES-CEACAM6, pIRES‑4‑1BBL and pIRES-CEACAM6-4-1BBL were constructed. The rats were administered subcutaneous injections of 1,2-dimethyl­hydrazine (DMH) once a week for 18 weeks. Eight weeks after the first injection, the rats were divided into the pIRES/SL3261, pIRES-4-1BBL/SL3261, pIRES-CEACAM6/SL3261 and pIRES-CEACAM6-4-1BBL/SL3261 groups, and fed with corresponding vaccine strains. The rats were then sacrificed, the number of colon tumors were recorded, and the Dukes' stage were evaluated. CD3, CD4, CD8, CD56, FOXP3 and CEACAM6 expression in tumor tissues was determined by immunohistochemical staining. Compared with the expression levels in the pIRES/SL3261 group, similar levels of CD3+, CD8+ and CD56+ expression were identified for the pIRES-CEACAM6/SL3261 group of rats. Additionally, a comparable number of tumors was detected in the pIRES-4-1BBL/SL3261 and pIRES-CEACAM6/SL3261 groups. By contrast, a significantly fewer number of tumors, albeit with a higher density of CD3+CD8+, CD56+ and a lower density of Foxp3+ tumor-infiltrating lymphocyte (TIL) cells was detected in the pIRES-CEACAM6-4-1BBL/SL3261 group of rats. The results indicated that vaccination with recombinant attenuated Salmonella harboring the CEACAM6 and 4-1BBL gene efficiently increased the number of CD3+CD8+ TIL and NK cells, decreased the number of FOXP3 cells and inhibited the development of DMH-induced colorectal cancer in rats.
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Copy and paste a formatted citation
Spandidos Publications style
Jin C, Liu Y, Zhu J, Xia T, Zhang B, Fei Y, Ma B, Ye J and Chen W: Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6. Oncol Rep 33: 2837-2844, 2015.
APA
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y. ... Chen, W. (2015). Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6. Oncology Reports, 33, 2837-2844. https://doi.org/10.3892/or.2015.3901
MLA
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y., Ma, B., Ye, J., Chen, W."Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6". Oncology Reports 33.6 (2015): 2837-2844.
Chicago
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y., Ma, B., Ye, J., Chen, W."Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6". Oncology Reports 33, no. 6 (2015): 2837-2844. https://doi.org/10.3892/or.2015.3901
Copy and paste a formatted citation
x
Spandidos Publications style
Jin C, Liu Y, Zhu J, Xia T, Zhang B, Fei Y, Ma B, Ye J and Chen W: Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6. Oncol Rep 33: 2837-2844, 2015.
APA
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y. ... Chen, W. (2015). Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6. Oncology Reports, 33, 2837-2844. https://doi.org/10.3892/or.2015.3901
MLA
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y., Ma, B., Ye, J., Chen, W."Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6". Oncology Reports 33.6 (2015): 2837-2844.
Chicago
Jin, C., Liu, Y., Zhu, J., Xia, T., Zhang, B., Fei, Y., Ma, B., Ye, J., Chen, W."Recombinant Salmonella-based CEACAM6 and 4-1BBL vaccine enhances T-cell immunity and inhibits the development of colorectal cancer in rats: In vivo effects of vaccine containing 4-1BBL and CEACAM6". Oncology Reports 33, no. 6 (2015): 2837-2844. https://doi.org/10.3892/or.2015.3901
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