Effects of emodin on the demethylation of tumor-suppressor genes in pancreatic cancer PANC-1 cells

  • Authors:
    • Hao Zhang
    • Liang Chen
    • He-Qi Bu
    • Qing-Jiang Yu
    • Dan-Dan Jiang
    • Feng-Ping Pan
    • Yu Wang
    • Dian-Lei Liu
    • Sheng-Zhang Lin
  • View Affiliations

  • Published online on: April 17, 2015     https://doi.org/10.3892/or.2015.3914
  • Pages: 3015-3023
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Abstract

Emodin, a natural anthraquinone derivative isolated from Rheum palmatum, has been reported to inhibit the growth of pancreatic cancer cells through different modes of action; yet, the detailed mechanism remains unclear. In the present study, we hypothesized that emodin exerts its antitumor effect by participating in the regulation of the DNA methylation level. Our research showed that emodin inhibited the growth of pancreatic cancer PANC-1 cells in a dose- and time-dependent manner. Dot-blot results showed that 40 µM emodin significantly inhibited genomic 5mC expression in the PANC-1 cells, and mRNA‑Seq showed that different concentrations of emodin could alter the gene expression profile in the PANC-1 cells. BSP confirmed that the methylation levels of P16, RASSF1A and ppENK were decreased, while concomitantly the unmethylated status was increased. RT-PCR and western blotting results confirmed that the low expression or absence of expression of mRNA and protein in the PANC-1 cells was re-expressed following treatment with emodin. In conclusion, our study for the first time suggests that emodin inhibits pancreatic cancer cell growth, which may be related to the demethylation of tumor-suppressor genes. The related mechanism may be through the inhibition of methyltransferase expression.

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June 2015
Volume 33 Issue 6

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Copy and paste a formatted citation
APA
Zhang, H., Chen, L., Bu, H., Yu, Q., Jiang, D., Pan, F. ... Lin, S. (2015). Effects of emodin on the demethylation of tumor-suppressor genes in pancreatic cancer PANC-1 cells. Oncology Reports, 33, 3015-3023. https://doi.org/10.3892/or.2015.3914
MLA
Zhang, H., Chen, L., Bu, H., Yu, Q., Jiang, D., Pan, F., Wang, Y., Liu, D., Lin, S."Effects of emodin on the demethylation of tumor-suppressor genes in pancreatic cancer PANC-1 cells". Oncology Reports 33.6 (2015): 3015-3023.
Chicago
Zhang, H., Chen, L., Bu, H., Yu, Q., Jiang, D., Pan, F., Wang, Y., Liu, D., Lin, S."Effects of emodin on the demethylation of tumor-suppressor genes in pancreatic cancer PANC-1 cells". Oncology Reports 33, no. 6 (2015): 3015-3023. https://doi.org/10.3892/or.2015.3914