Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray

  • Authors:
    • Xiang Ding
    • Yiling Hou
    • Yuanxiu Zhu
    • Panpan Wang
    • Lei Fu
    • Hongqing Zhu
    • Nan Zhang
    • Hang Qin
    • Wei Qu
    • Fang Wang
    • Wanru Hou
  • View Affiliations

  • Published online on: April 21, 2015     https://doi.org/10.3892/or.2015.3921
  • Pages: 3162-3170
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Abstract

A novel heteropolysaccharide from the fruiting bodies of Gomphus clavatus Gray was isolated through Sephadex G-200 and DEAE-cellulose columns. The Gomphus clavatus Gray polysaccharide (GCG-1) was mainly composed of β-D-glucosepyranose (β-D-Glu) and α-D-galactopyranose (α-D-Gal) in a ratio of 3:2 and had a molecular weight of ~50,000 Da. The structure of GCG-1 was investigated by a combination of total hydrolysis, gas chromatography-mass spectrometry, methylation analysis, nuclear magnetic resonance spectroscopy and infrared spectra. The results indicated that GCG-1 had a backbone of (1→4)-β-D-glucosepyranose residues with branches at O-6 and the branches consisted of two with (1→3)-α-D-galactopyranose residue. Antioxidation test in vitro showed that it possessedstrong free radical scavenging activity, which may be comparable to vitamin C and butylated hydroxytoluene. GCG-1 also induced the apoptosis of HepG-2 cells and affected the mRNA expression of various housekeeping genes in the HepG-2 cells. The results indicated that Gomphus clavatus Gray may be an ideal sources for antioxidant and anticancer agents.
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June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ding X, Hou Y, Zhu Y, Wang P, Fu L, Zhu H, Zhang N, Qin H, Qu W, Wang F, Wang F, et al: Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray. Oncol Rep 33: 3162-3170, 2015
APA
Ding, X., Hou, Y., Zhu, Y., Wang, P., Fu, L., Zhu, H. ... Hou, W. (2015). Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray. Oncology Reports, 33, 3162-3170. https://doi.org/10.3892/or.2015.3921
MLA
Ding, X., Hou, Y., Zhu, Y., Wang, P., Fu, L., Zhu, H., Zhang, N., Qin, H., Qu, W., Wang, F., Hou, W."Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray". Oncology Reports 33.6 (2015): 3162-3170.
Chicago
Ding, X., Hou, Y., Zhu, Y., Wang, P., Fu, L., Zhu, H., Zhang, N., Qin, H., Qu, W., Wang, F., Hou, W."Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray". Oncology Reports 33, no. 6 (2015): 3162-3170. https://doi.org/10.3892/or.2015.3921