miR-128-3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients

  • Authors:
    • Chao-Yuan Huang
    • Xin-Ping Huang
    • Ji-Ye Zhu
    • Zhi-Gang Chen
    • Xian-Jian Li
    • Xue-Hui Zhang
    • Shan Huang
    • Jian-Bo He
    • Fang Lian
    • Yin-Nong Zhao
    • Guo-Bin Wu
  • View Affiliations

  • Published online on: April 28, 2015     https://doi.org/10.3892/or.2015.3936
  • Pages: 2889-2898
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Abstract

microRNAs (miRNAs) are known to be involved in the pathogenesis of hepatocellular carcinoma (HCC). miR-128-3p was recently reported to be deregulated in several types of cancer. However, the biological function and potential mechanisms of miR-128-3p in HCC remain unknown. In the present study, we found that miR-128-3p was frequently downregulated in HCC tissues and cell lines by qRT-PCR analysis. Moreover, functional assays showed that overexpression of miR-128-3p markedly suppressed HCC cell proliferation by inducing G1 phase cell arrest and migration. Mechanistically, miR‑128-3p was confirmed to regulate PIK3R1 (p85α) expression thereby suppressing phosphatidylinositol 3-kinase (PI3K)/AKT pathway activation using qRT-PCR and western blot analysis. Furthermore, correlation analysis and Kaplan‑Meier estimates revealed an inverse correlation between miR-128-3p and p85α as well as a shorter disease‑free survival (DFS) period after HCC resection in patients with low miR-128-3p expression. Hence, we conclude that miR‑128-3p, which is frequently downregulated in HCC, inhibits HCC progression by regulating PIK3R1 and PI3K/AKT activation, and is a prognostic marker for HCC patients.
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June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang C, Huang X, Zhu J, Chen Z, Li X, Zhang X, Huang S, He J, Lian F, Zhao Y, Zhao Y, et al: miR-128-3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients. Oncol Rep 33: 2889-2898, 2015
APA
Huang, C., Huang, X., Zhu, J., Chen, Z., Li, X., Zhang, X. ... Wu, G. (2015). miR-128-3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients. Oncology Reports, 33, 2889-2898. https://doi.org/10.3892/or.2015.3936
MLA
Huang, C., Huang, X., Zhu, J., Chen, Z., Li, X., Zhang, X., Huang, S., He, J., Lian, F., Zhao, Y., Wu, G."miR-128-3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients". Oncology Reports 33.6 (2015): 2889-2898.
Chicago
Huang, C., Huang, X., Zhu, J., Chen, Z., Li, X., Zhang, X., Huang, S., He, J., Lian, F., Zhao, Y., Wu, G."miR-128-3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients". Oncology Reports 33, no. 6 (2015): 2889-2898. https://doi.org/10.3892/or.2015.3936