High expression of RELM-α correlates with poor prognosis and promotes angiogenesis in gastric cancer Corrigendum in /10.3892/or.2018.6933

Chen,Ping; Zhao,Deshou; Wang,Weiyi; Zhang,Yongping; Yuan,Yaozong; Wang,Lifu; Wu,Yunlin

doi:10.3892/or.2015.3943

High expression of RELM-α correlates with poor prognosis and promotes angiogenesis in gastric cancer

Corrigendum in: /10.3892/or.2018.6933

Authors:
- Ping Chen
- Deshou Zhao
- Weiyi Wang
- Yongping Zhang
- Yaozong Yuan
- Lifu Wang
- Yunlin Wu
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Affiliations: Department of Gastroenterology, Ruijin Hospital North, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China, Department of Laboratory, Second Hospital Affiliated to Lanzhou University, Lanzhou, Gansu 746000, P.R. China, Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
Published online on: April 30, 2015 https://doi.org/10.3892/or.2015.3943
Pages: 77-86

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Abstract

Accumulating evidence indicates that resistin‑like molecule-α (RELM-α) is involved in angiogenesis, while the clinical significance and the exact role of RELM-α in gastric cancer remain obscure. The aim of the present study was to evaluate the clinical significance of RELM-α in gastric cancer, and to investigate its effective mechanisms in order to identify a potential therapeutic target. The expression levels of RELM-α in 92 gastric cancer and adjacent normal tissues were investigated and the relationship between RELM-α expression and the clinicopathological characteristics was explored. To investigate the potential role of RELM-α in gastric cancer cell biological behavior, the cell proliferation, migration and invasion assays were conducted using two gastric cancer cell lines (SGC7901 and MKN45). We also assessed whether RELM-α gene silencing modulates angiogenesis using small interference RNA in cancer cell lines, and investigated its effect on nuclear factor (NF)-κB activation and vascular endothelial growth factor (VEGF) and MMP-9 expression. Contrasting sharply with the strong RELM-α-positive tumors, adjacent normal tissues and cell lines exhibited negative or weakly positive expression (P<0.01). High expression level of RELM-α was associated with advanced stage and tumor size (P<0.01). The silencing of RELM-α expression by Ad5/F35-siRNA treatment significantly inhibited cell migratory and invasive ability in SGC7901 and MKN45 gastric cancer cells compared with the control and Ad5/F35 vector-transfected cell lines (P<0.01). However, the silencing of RELM-α expression also significantly blocked NF-κB activation and attenuated VEGF and MMP-9 expression. The data demonstrated that RELM-α is a promising novel biomarker of angiogenesis in patients with gastric cancer. The study identified that the silencing of RELM-α expression may regulate the proliferation, invasion and migration of gastric cancer cells by targeting VEGF/MMP-9, and the mechanism involved tissue angiogenesis via the NF-κB signaling pathway.

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