MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells

  • Authors:
    • Jun Zhou
    • Ming Xie
    • Ying Shi
    • Baihua Luo
    • Guanghui Gong
    • Juanni Li
    • Junpu Wang
    • Wenjian Zhao
    • Yuan Zi
    • Xiaoying Wu
    • Jifang Wen
  • View Affiliations

  • Published online on: May 5, 2015     https://doi.org/10.3892/or.2015.3952
  • Pages: 111-120
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Abstract

The overall goal of the present study was to find and validate unidentified miRNAs that regulate epithelial-mesenchymal transition (EMT) and proliferation in ovarian cancer. Furthermore, we demonstrate that the high expression of miR-153 in human epithelial ovarian cancer (EOC) is associated with better survival. The mean expression level of miR-153 in ovarian cancer was significantly lower than in the adjacent carcinoma tissue. In the present study, we report that miR-153 are negative regulators of SET7 and ZEB2, miR-153 regulates SET7/ZEB2 expression and promotes SET7/ZEB2 mRNA degradation. Further, confirmed by reporter assays, SET7/ZEB2 are downstream targets of miR-153 directly bound to the 3' untranslated region (3'-UTR). Clone formation and wound-healing assay as well as Transwell assay proved that silencing of SET7 or ZEB2 partially abolished the enhancement of cell proliferation and invasion induced by downregulated miR-153. SET7 and ZEB2 are negatively correlated with miR-153 expression in human ovarian cancer and indicated a worse survival. Considering the role of SET7 and ZEB2 in EOC, it is important to clarify how the expression of SET7 and ZEB2 are regulated. Based on our results miR-153 inhibits proliferation and suppresses EMT and the invasive potential of ovarian cancer cells through downregulation of SET7 and ZEB2, supporting the pursuit of miR-153 as a potential target for ovarian cancer intervention.
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July-2015
Volume 34 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou J, Xie M, Shi Y, Luo B, Gong G, Li J, Wang J, Zhao W, Zi Y, Wu X, Wu X, et al: MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells. Oncol Rep 34: 111-120, 2015
APA
Zhou, J., Xie, M., Shi, Y., Luo, B., Gong, G., Li, J. ... Wen, J. (2015). MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells. Oncology Reports, 34, 111-120. https://doi.org/10.3892/or.2015.3952
MLA
Zhou, J., Xie, M., Shi, Y., Luo, B., Gong, G., Li, J., Wang, J., Zhao, W., Zi, Y., Wu, X., Wen, J."MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells". Oncology Reports 34.1 (2015): 111-120.
Chicago
Zhou, J., Xie, M., Shi, Y., Luo, B., Gong, G., Li, J., Wang, J., Zhao, W., Zi, Y., Wu, X., Wen, J."MicroRNA-153 functions as a tumor suppressor by targeting SET7 and ZEB2 in ovarian cancer cells". Oncology Reports 34, no. 1 (2015): 111-120. https://doi.org/10.3892/or.2015.3952