miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1

Nie,Jihua; Ge,Xin; Geng,Yangyang; Cao,Han; Zhu,Wei; Jiao,Yang; Wu,Jinchang; Zhou,Jundong; Cao,Jianping

doi:10.3892/or.2015.3962

miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1

Authors:
- Jihua Nie
- Xin Ge
- Yangyang Geng
- Han Cao
- Wei Zhu
- Yang Jiao
- Jinchang Wu
- Jundong Zhou
- Jianping Cao
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Affiliations: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China, The Core Laboratory of Suzhou Cancer Center and Department of Radiotherapy of Suzhou Municipal Hospital, Suzhou, Jiangsu 215001, P.R. China
Published online on: May 7, 2015 https://doi.org/10.3892/or.2015.3962
Pages: 311-317

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Abstract

Esophageal squamous cell carcinoma (ESCC), one of the most common gastrointestinal tumors, is known for its high mortality rate. microRNAs (miRNAs) have been reported to play important regulatory roles in cancer metastasis and progression. miR-34a has been demonstrated to be associated with the development of and metastasis in certain types of cancer via various target genes, but its function and targets in ESCC are unknown. The aim of this study was to examine whether the expression of miR-34a was significantly decreased in ESCC tissues, compared with normal esophageal tissues using RT-PCR and western blot analysis. The results showed that miR-34a overexpression increased apoptosis and decreased clonogenic formation, but inhibited invasion and migration in ESCC cells by suppressing MMP-2 and -9 expression. Yin Yang-1 (YY1), a widely distributed transcription factor that belongs to the GLI-Kruppel class of zinc finger proteins, was found to be a direct target of miR-34a in ESCC cell lines. Rescue experiments indicated that the suppressive effect of miR-34a on invasion and migration was mediated by activating YY1 expression. Results of the present study showed that miR-34a is associated with ESCC migration and provides a potential therapeutic and diagnostic target for ESCC.

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