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Article

Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer

  • Authors:
    • Zhaojin Yu
    • Mingli Sun
    • Feng Jin
    • Qinghuan Xiao
    • Miao He
    • Huizhe Wu
    • Jie Ren
    • Lin Zhao
    • Haishan Zhao
    • Weifan Yao
    • Fengping Shan
    • Yaming Cao
    • Minjie Wei
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning 110122, P.R. China, Department of Breast Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning 110122, P.R. China, Department of Immunology, School of Basic Medical Science, China Medical University, Shenyang, Liaoning 110122, P.R. China
  • Pages: 165-174
    |
    Published online on: May 8, 2015
       https://doi.org/10.3892/or.2015.3967
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Abstract

Ezrin and E-cadherin have been known to play a role in tumor metastasis. However, the association between the expression of ezrin and E-cadherin in breast cancer patients remains unclear. In the present study, we investigated the expression of ezrin and E-cadherin in 275 breast cancer and 80 control patients with benign hyperplasia, using tissue microarray‑based immunohistochemistry (IHC). Ezrin expression was higher, while that of E-cadherin was lower in breast cancer than in control samples. Ezrin expression was negatively correlated with E-cadherin expression in a subpopulation of breast cancer patients with a high expression of ezrin [ezrin(high)] and a low expression of E-cadherin [E-cad(low)]. The cytoplasmic expression of E-cadherin [E-cad(c)] occurred significantly more frequently in ezrin(high) breast cancer than in ezrin(low) breast cancer. The expression level of ezrin was significantly higher in breast cancer with E-cad(c) than that with a membrane expression of E-cadherin [E-cad(m)]. Ezrin(high) or E-cad(low) expression was more associated with lymph node metastasis, and shorter overall survival (OS) and disease-free survival (DFS) in breast cancer patients. E-cad(c) was more associated with lymph node metastasis and shorter OS and DFS compared with E-cad(m). The combined expression of ezrin(high) and E-cad(low) or ezrin(high) and ezrin(c) was more associated with lymph node metastasis and poor prognosis. In addition, the multivariate Cox regression analysis revealed that lymph node metastasis and ezrin(high) expression were independent prognostic factors for shorter OS and DFS in breast cancer patients. The results of the present study suggested that ezrin promotes breast cancer metastasis via the regulation of E-cadherin expression.
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1 

Lu J, Steeg PS, Price JE, Krishnamurthy S, Mani SA, Reuben J, Cristofanilli M, Dontu G, Bidaut L, Valero V, et al: Breast cancer metastasis: Challenges and opportunities. Cancer Res. 69:4951–4953. 2009. View Article : Google Scholar : PubMed/NCBI

2 

O’Shaughnessy J: Extending survival with chemotherapy in metastatic breast cancer. Oncologist. 10(Suppl 3): S20–S29. 2005. View Article : Google Scholar

3 

Friedl P and Wolf K: Tumour-cell invasion and migration: Diversity and escape mechanisms. Nat Rev Cancer. 3:362–374. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Tsukita S and Yonemura S: ERM (ezrin/radixin/moesin) family: From cytoskeleton to signal transduction. Curr Opin Cell Biol. 9:70–75. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Nestl A, Von Stein OD, Zatloukal K, Thies WG, Herrlich P, Hofmann M and Sleeman JP: Gene expression patterns associated with the metastatic phenotype in rodent and human tumors. Cancer Res. 61:1569–1577. 2001.PubMed/NCBI

6 

Yu H, Zhang Y, Ye L and Jiang WG: The FERM family proteins in cancer invasion and metastasis. Front Biosci. 16:1536–1550. 2011. View Article : Google Scholar

7 

Yu Y, Khan J, Khanna C, Helman L, Meltzer PS and Merlino G: Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat Med. 10:175–181. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Khanna C, Wan X, Bose S, Cassaday R, Olomu O, Mendoza A, Yeung C, Gorlick R, Hewitt SM and Helman LJ: The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med. 10:182–186. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Meng Y, Lu Z, Yu S, Zhang Q, Ma Y and Chen J: Ezrin promotes invasion and metastasis of pancreatic cancer cells. J Transl Med. 8:612010. View Article : Google Scholar : PubMed/NCBI

10 

Kang YK, Hong SW, Lee H and Kim WH: Prognostic implications of ezrin expression in human hepatocellular carcinoma. Mol Carcinog. 49:798–804. 2010.PubMed/NCBI

11 

Saito S, Yamamoto H, Mukaisho K, Sato S, Higo T, Hattori T, Yamamoto G and Sugihara H: Mechanisms underlying cancer progression caused by ezrin overexpression in tongue squamous cell carcinoma. PLoS One. 8:e548812013. View Article : Google Scholar : PubMed/NCBI

12 

Gschwantler-Kaulich D, Natter C, Steurer S, Walter I, Thomas A, Salama M and Singer CF: Increase in ezrin expression from benign to malignant breast tumours. Cell Oncol. 36:485–491. 2013. View Article : Google Scholar

13 

Halon A, Donizy P, Surowiak P and Matkowski R: ERM/Rho protein expression in ductal breast cancer: A 15 year follow-up. Cell Oncol. 36:181–190. 2013. View Article : Google Scholar

14 

Elliott BE, Meens JA, SenGupta SK, Louvard D and Arpin M: The membrane cytoskeletal crosslinker ezrin is required for metastasis of breast carcinoma cells. Breast Cancer Res. 7:R365–R373. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, Liu P, Song A, Gang C, Han Z, et al: Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells. Cancer Lett. 261:55–63. 2008. View Article : Google Scholar

16 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: At the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Jeanes A, Gottardi CJ and Yap AS: Cadherins and cancer: How does cadherin dysfunction promote tumor progression? Oncogene. 27:6920–6929. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Foschini MP, Leonardi E, Eusebi LH, Farnedi A, Poli T, Tarsitano A, Cocchi R, Marchetti C, Gentile L, Sesenna E, et al: Podoplanin and E-cadherin expression in preoperative incisional biopsies of oral squamous cell carcinoma is related to lymph node metastases. Int J Surg Pathol. 21:133–141. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Wang C, Jiang K, Kang X, Gao D, Sun C, Li Y, Sun L, Zhang S, Liu X, Wu W, et al: Tumor-derived secretory clusterin induces epithelial-mesenchymal transition and facilitates hepatocellular carcinoma metastasis. Int J Biochem Cell Biol. 44:2308–2320. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Wang WS, Yu SL, Yang XS, Chang SD and Hou JQ: Expression and significance of twist and E-cadherin in ovarian cancer tissues. Asian Pac J Cancer Prev. 14:669–672. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Stanczak A, Stec R, Bodnar L, Olszewski W, Cichowicz M, Kozlowski W, Szczylik C, Pietrucha T, Wieczorek M and Lamparska-Przybysz M: Prognostic significance of Wnt-1, β-catenin and E-cadherin expression in advanced colorectal carcinoma. Pathol Oncol Res. 17:955–963. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Rakha EA, Patel A, Powe DG, Benhasouna A, Green AR, Lambros MB, Reis-Filho JS and Ellis IO: Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast. Am J Surg Pathol. 34:1472–1479. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Oka H, Shiozaki H, Kobayashi K, Inoue M, Tahara H, Kobayashi T, Takatsuka Y, Matsuyoshi N, Hirano S, Takeichi M, et al: Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis. Cancer Res. 53:1696–1701. 1993.PubMed/NCBI

24 

Chen MJ, Gao XJ, Xu LN, Liu TF, Liu XH and Liu LX: Ezrin is required for epithelial-mesenchymal transition induced by TGF-β1 in A549 cells. Int J Oncol. 45:1515–1522. 2014.PubMed/NCBI

25 

Fang Y, Wei J, Cao J, Zhao H, Liao B, Qiu S, Wang D, Luo J and Chen W: Protein expression of ZEB2 in renal cell carcinoma and its prognostic significance in patient survival. PLoS One. 8:e625582013. View Article : Google Scholar : PubMed/NCBI

26 

Zhu W, Cai MY, Tong ZT, Dong SS, Mai SJ, Liao YJ, Bian XW, Lin MC, Kung HF, Zeng YX, et al: Overexpression of EIF5A2 promotes colorectal carcinoma cell aggressiveness by upregulating MTA1 through C-myc to induce epithelial-mesenchymaltransition. Gut. 61:562–575. 2012. View Article : Google Scholar

27 

Sarrió D, Rodríguez-Pinilla SM, Dotor A, Calero F, Hardisson D and Palacios J: Abnormal ezrin localization is associated with clinicopathological features in invasive breast carcinomas. Breast Cancer Res Treat. 98:71–79. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Pujuguet P, Del Maestro L, Gautreau A, Louvard D and Arpin M: Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol Biol Cell. 14:2181–2191. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Siitonen SM, Kononen JT, Helin HJ, Rantala IS, Holli KA and Isola JJ: Reduced E-cadherin expression is associated with invasiveness and unfavorable prognosis in breast cancer. Am J Clin Pathol. 105:394–402. 1996.PubMed/NCBI

30 

Yoshida R, Kimura N, Harada Y and Ohuchi N: The loss of E-cadherin, α- and β-catenin expression is associated with metastasis and poor prognosis in invasive breast cancer. Int J Oncol. 18:513–520. 2001.PubMed/NCBI

31 

Brzozowska A, Sodolski T, Duma D, Mazurkiewicz T and Mazurkiewicz M: Evaluation of prognostic parameters of E-cadherin status in breast cancer treatment. Ann Agric Environ Med. 19:541–546. 2012.PubMed/NCBI

32 

Kowalski PJ, Rubin MA and Kleer CG: E-cadherin expression in primary carcinomas of the breast and its distant metastases. Breast Cancer Res. 5:R217–R222. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Zhang Y, Zhao Y, Jiang G, Zhang X, Zhao H, Wu J, Xu K and Wang E: Impact of p120-catenin isoforms 1A and 3A on epithelial mesenchymal transition of lung cancer cells expressing E-cadherin in different subcellular locations. PLoS One. 9:e880642014. View Article : Google Scholar : PubMed/NCBI

34 

Lapyckyj L, Castillo LF, Matos ML, Gabrielli NM, Lüthy IA and Vazquez-Levin MH: Expression analysis of epithelial cadherin and related proteins in IBH-6 and IBH-4 human breast cancer cell lines. J Cell Physiol. 222:596–605. 2010.

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Copy and paste a formatted citation
Spandidos Publications style
Yu Z, Sun M, Jin F, Xiao Q, He M, Wu H, Ren J, Zhao L, Zhao H, Yao W, Yao W, et al: Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer. Oncol Rep 34: 165-174, 2015.
APA
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H. ... Wei, M. (2015). Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer. Oncology Reports, 34, 165-174. https://doi.org/10.3892/or.2015.3967
MLA
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H., Ren, J., Zhao, L., Zhao, H., Yao, W., Shan, F., Cao, Y., Wei, M."Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer". Oncology Reports 34.1 (2015): 165-174.
Chicago
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H., Ren, J., Zhao, L., Zhao, H., Yao, W., Shan, F., Cao, Y., Wei, M."Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer". Oncology Reports 34, no. 1 (2015): 165-174. https://doi.org/10.3892/or.2015.3967
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Z, Sun M, Jin F, Xiao Q, He M, Wu H, Ren J, Zhao L, Zhao H, Yao W, Yao W, et al: Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer. Oncol Rep 34: 165-174, 2015.
APA
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H. ... Wei, M. (2015). Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer. Oncology Reports, 34, 165-174. https://doi.org/10.3892/or.2015.3967
MLA
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H., Ren, J., Zhao, L., Zhao, H., Yao, W., Shan, F., Cao, Y., Wei, M."Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer". Oncology Reports 34.1 (2015): 165-174.
Chicago
Yu, Z., Sun, M., Jin, F., Xiao, Q., He, M., Wu, H., Ren, J., Zhao, L., Zhao, H., Yao, W., Shan, F., Cao, Y., Wei, M."Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer". Oncology Reports 34, no. 1 (2015): 165-174. https://doi.org/10.3892/or.2015.3967
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