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Article

P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway

  • Authors:
    • Jiyi Xia
    • Xiaolan Yu
    • Li Tang
    • Gang Li
    • Tao He
  • View Affiliations / Copyright

    Affiliations: Research Center for Drug and Functional Food, Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Department of Obstetrics and Gynecology, The Affiliated TCM Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Experimental Medicine Center, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China, Department of Biochemistry, Luzhou Medical College, Luzhou, Sichuan 646000, P.R. China
  • Pages: 103-110
    |
    Published online on: May 13, 2015
       https://doi.org/10.3892/or.2015.3979
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Abstract

Purinergic signaling has been implicated in the regulation of many cellular processes. A high concentration of ATP has been observed in the tumor microenvironment, suggesting a possible role of extracellular ATP in tumor progression. The P2X7 receptor, which belongs to the ligand-gated ion channel receptor family, is involved in tumor development and metastasis. In the present study, we found that extracellular ATP stimulated the invasion and migration of human T47D breast cancer cells, in a dose-dependent manner. BzATP (ATP analogue), but not ADP, also promoted invasion and migration. We further found that the P2X7 receptor was highly expressed in the T47D cells. After knockdown of the P2X7 receptor, ATP-stimulated invasion and migration were markedly inhibited. Moreover, activation of the P2X7 receptor by ATP downregulated the protein level of E-cadherin and upregulated the production of MMP-13. In addition, ATP time-dependently induced the activation of AKT via the P2X7 receptor, and the AKT pathway was required for the ATP-mediated invasion and migration. Taken together, our results revealed that activation of the P2X7 receptor by ATP promotes breast cancer cell invasion and migration, possibly via activation of the AKT pathway and regulation of E-cadherin and MMP-13 expression. Therefore, the P2X7 receptor may be a useful therapeutic target for the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xia J, Yu X, Tang L, Li G and He T: P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway. Oncol Rep 34: 103-110, 2015.
APA
Xia, J., Yu, X., Tang, L., Li, G., & He, T. (2015). P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway. Oncology Reports, 34, 103-110. https://doi.org/10.3892/or.2015.3979
MLA
Xia, J., Yu, X., Tang, L., Li, G., He, T."P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway". Oncology Reports 34.1 (2015): 103-110.
Chicago
Xia, J., Yu, X., Tang, L., Li, G., He, T."P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway". Oncology Reports 34, no. 1 (2015): 103-110. https://doi.org/10.3892/or.2015.3979
Copy and paste a formatted citation
x
Spandidos Publications style
Xia J, Yu X, Tang L, Li G and He T: P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway. Oncol Rep 34: 103-110, 2015.
APA
Xia, J., Yu, X., Tang, L., Li, G., & He, T. (2015). P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway. Oncology Reports, 34, 103-110. https://doi.org/10.3892/or.2015.3979
MLA
Xia, J., Yu, X., Tang, L., Li, G., He, T."P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway". Oncology Reports 34.1 (2015): 103-110.
Chicago
Xia, J., Yu, X., Tang, L., Li, G., He, T."P2X7 receptor stimulates breast cancer cell invasion and migration via the AKT pathway". Oncology Reports 34, no. 1 (2015): 103-110. https://doi.org/10.3892/or.2015.3979
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