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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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July-2015 Volume 34 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells

  • Authors:
    • Qiheng Gou
    • Yuxin Xie
    • Lei Liu
    • Keqi Xie
    • Yanxia Wu
    • Qianqian Wang
    • Zhu Wang
    • Ping Li

  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Anesthesiology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China, Laboratory of Molecular Diagnosis of Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 251-257
    |
    Published online on: May 14, 2015
       https://doi.org/10.3892/or.2015.3980
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Abstract

DNA double-strand breaks (DSBs) induced by ionizing radiation (IR) are among the most cytotoxic types of DNA damage. The DNA damage response (DDR) may be a reason for the cancer cell resistance to radiotherapy using IR. Identified as critical upstream mediators of the phosphorylation of ataxia telangiectasia‑mutated (ATM) pathway, mediator of DNA damage checkpoint 1 (MDC1) and p53-binding proteins 1 (53BP1) may affect the radiosensitivity of tumor cells. In the present study, we generated two HEP-2 cell lines with a stable knockdown of MDC1 or 53BP1 with short hairpin RNA (shRNA), respectively, and investigated the effect of MDC1 and 53BP1 on cell radiosensitivity, cell cycle distribution and the formation of cell foci. Downregulation of the two proteins reduced the number of clonogenic cells that treated with IR. Accumulation of G2/M phase cells was detected after the MDC1 and 53BP1 downregulation. These results indicated that the expression of MDC1 or 53BP1 limited tumor cell sensitivity to radiotherapy and may play an important role in the DNA repair progression. Furthermore, the MDC1 foci was identified and presented in the 53BP1-inhibited cells. By contrast, the 53BP1 foci was absent from the MDC1‑inhibited cells. The results confirmed that the recruitment of 53BP1 into the foci occurred in an MDC1-dependent manner.

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Copy and paste a formatted citation
Spandidos Publications style
Gou Q, Xie Y, Liu L, Xie K, Wu Y, Wang Q, Wang Z and Li P: Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells. Oncol Rep 34: 251-257, 2015.
APA
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q. ... Li, P. (2015). Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells. Oncology Reports, 34, 251-257. https://doi.org/10.3892/or.2015.3980
MLA
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q., Wang, Z., Li, P."Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells". Oncology Reports 34.1 (2015): 251-257.
Chicago
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q., Wang, Z., Li, P."Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells". Oncology Reports 34, no. 1 (2015): 251-257. https://doi.org/10.3892/or.2015.3980
Copy and paste a formatted citation
x
Spandidos Publications style
Gou Q, Xie Y, Liu L, Xie K, Wu Y, Wang Q, Wang Z and Li P: Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells. Oncol Rep 34: 251-257, 2015.
APA
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q. ... Li, P. (2015). Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells. Oncology Reports, 34, 251-257. https://doi.org/10.3892/or.2015.3980
MLA
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q., Wang, Z., Li, P."Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells". Oncology Reports 34.1 (2015): 251-257.
Chicago
Gou, Q., Xie, Y., Liu, L., Xie, K., Wu, Y., Wang, Q., Wang, Z., Li, P."Downregulation of MDC1 and 53BP1 by short hairpin RNA enhances radiosensitivity in laryngeal carcinoma cells". Oncology Reports 34, no. 1 (2015): 251-257. https://doi.org/10.3892/or.2015.3980
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