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Article

MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1

Retraction in: /10.3892/or.2021.8098
  • Authors:
    • Ying Shi
    • Jianjun Huang
    • Jun Zhou
    • Ying Liu
    • Xiaodan Fu
    • Yimin Li
    • Gang Yin
    • Jifang Wen
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan 410013, P.R. China, The Second Department of Orthopedics, The First Affiliated Hospital of Jishou University, Jishou, Hunan 416000, P.R. China
  • Pages: 399-406
    |
    Published online on: May 19, 2015
       https://doi.org/10.3892/or.2015.3986
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Abstract

MicroRNAs (miRs) play crucial roles in tumorigenesis by directly suppressing the protein expression levels of their target genes. miR-204 has been suggested to act as a tumor suppressor in several types of human cancer. However, the exact role of miR-204 in osteosarcoma (OS) remains undetermined. In the present study, we aimed to investigate the effects of miR-204 on OS cell proliferation, migration and invasion, as well as the underlying molecular mechanisms. We found that the expression of miR-204 was frequently downregulated in four OS cell lines compared to the level in normal human osteoblast cells. Moreover, overexpression of miR-204 significantly inhibited the proliferation, migration and invasion of OS cells. Based on bioinformatics prediction and a luciferase reporter assay, we identified Sirtuin 1 (Sirt1) as a direct target gene of miR-204 in OS Saso-2 cells. Moreover, the protein expression of Sirt1 was negatively mediated by miR-204 in the OS cells. siRNA-mediated knockdown of Sirt1 also inhibited the proliferation, migration and invasion of the OS cells. Moreover, overexpression of Sirt1 reversed the inhibitory effect of miR-204 overexpression on the proliferation, migration and invasion of the OS cells. In addition, after miR-204 overexpression or Sirt1 knockdown in OS cells, the expression of E-cadherin was increased, while the N-cadherin protein level was reduced. Based on these findings, we suggest that miR-204 inhibits the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells by directly targeting Sirt1.
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Copy and paste a formatted citation
Spandidos Publications style
Shi Y, Huang J, Zhou J, Liu Y, Fu X, Li Y, Yin G and Wen J: MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098. Oncol Rep 34: 399-406, 2015.
APA
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y. ... Wen, J. (2015). MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098. Oncology Reports, 34, 399-406. https://doi.org/10.3892/or.2015.3986
MLA
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y., Yin, G., Wen, J."MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098". Oncology Reports 34.1 (2015): 399-406.
Chicago
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y., Yin, G., Wen, J."MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098". Oncology Reports 34, no. 1 (2015): 399-406. https://doi.org/10.3892/or.2015.3986
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Huang J, Zhou J, Liu Y, Fu X, Li Y, Yin G and Wen J: MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098. Oncol Rep 34: 399-406, 2015.
APA
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y. ... Wen, J. (2015). MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098. Oncology Reports, 34, 399-406. https://doi.org/10.3892/or.2015.3986
MLA
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y., Yin, G., Wen, J."MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098". Oncology Reports 34.1 (2015): 399-406.
Chicago
Shi, Y., Huang, J., Zhou, J., Liu, Y., Fu, X., Li, Y., Yin, G., Wen, J."MicroRNA-204 inhibits proliferation, migration, invasion and epithelial-mesenchymal transition in osteosarcoma cells via targeting Sirtuin 1 Retraction in /10.3892/or.2021.8098". Oncology Reports 34, no. 1 (2015): 399-406. https://doi.org/10.3892/or.2015.3986
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