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Article

miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy

  • Authors:
    • Wenjun Yin
    • Yuehua Nie
    • Zhiwei Zhang
    • Liming Xie
    • Xiusheng He
  • View Affiliations / Copyright

    Affiliations: Cancer Research Institute of Medical College, University of Southern China, University Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province, Hengyang, Hunan 421001, P.R. China, Department of Radiation Oncology, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Chemotherapy Oncology, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 368-374
    |
    Published online on: May 19, 2015
       https://doi.org/10.3892/or.2015.3996
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Abstract

Mounting evidence suggests that microRNAs (miRNAs) play important roles in the development of cancer by targeting expression of tumor-related genes. In the present study, downregulation of miR-193b was observed in hepatocellular carcinoma (HCC) tissues and HCC cell lines by quantitative RT-PCR analyses, suggesting that miR-193b is a tumor-suppressor in HCC. More importantly, miR-193b significantly enhanced the cytotoxicity of cisplatin in HepG2 cells by targeting Mcl-1. Knockdown of the Mcl-1 gene by specific siRNA exhibited a function similar to miR-193b on sensitizing HepG2 cells to cisplatin-inducing cytotoxicity. Furthermore, the miR-193b-induced sensitization of HepG2 cells to cisplatin cytotoxicity was abolished by the transfection of Mcl-1 expression plasmid that lacked the 3'-untranslated region (3'-UTR). In addition, activation of caspase-3 was needed for sensitization by miR-193b to cisplatin-mediated cell death. Thus, the present study revealed the downregulation of miR-193b in HCC cells and illustrated a synergistic effect on cisplatin-induced apoptosis by targeting Mcl-1.
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Copy and paste a formatted citation
Spandidos Publications style
Yin W, Nie Y, Zhang Z, Xie L and He X: miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy. Oncol Rep 34: 368-374, 2015.
APA
Yin, W., Nie, Y., Zhang, Z., Xie, L., & He, X. (2015). miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy. Oncology Reports, 34, 368-374. https://doi.org/10.3892/or.2015.3996
MLA
Yin, W., Nie, Y., Zhang, Z., Xie, L., He, X."miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy". Oncology Reports 34.1 (2015): 368-374.
Chicago
Yin, W., Nie, Y., Zhang, Z., Xie, L., He, X."miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy". Oncology Reports 34, no. 1 (2015): 368-374. https://doi.org/10.3892/or.2015.3996
Copy and paste a formatted citation
x
Spandidos Publications style
Yin W, Nie Y, Zhang Z, Xie L and He X: miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy. Oncol Rep 34: 368-374, 2015.
APA
Yin, W., Nie, Y., Zhang, Z., Xie, L., & He, X. (2015). miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy. Oncology Reports, 34, 368-374. https://doi.org/10.3892/or.2015.3996
MLA
Yin, W., Nie, Y., Zhang, Z., Xie, L., He, X."miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy". Oncology Reports 34.1 (2015): 368-374.
Chicago
Yin, W., Nie, Y., Zhang, Z., Xie, L., He, X."miR-193b acts as a cisplatin sensitizer via the caspase-3-dependent pathway in HCC chemotherapy". Oncology Reports 34, no. 1 (2015): 368-374. https://doi.org/10.3892/or.2015.3996
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