Ligularia fischeri inhibits endothelial cell proliferation, invasion and tube formation through the inactivation of mitogenic signaling pathways and regulation of vascular endothelial cadherin distribution and matrix metalloproteinase expression

  • Authors:
    • Jae Hyeon Kim
    • Hyeon-Ju Kim
    • Jin-Kyu Kim
    • Eun-Kyung Ahn
    • Hye-Jin Ko
    • Young-Rak Cho
    • Sang-Jin Lee
    • Gyu-Un Bae
    • Yong Kee Kim
    • Jong Woo Park
    • Joa Sub Oh
    • Dong-Wan Seo
  • View Affiliations

  • Published online on: May 20, 2015     https://doi.org/10.3892/or.2015.4000
  • Pages: 221-226
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Abstract

Ligularia fischeri (LF) has been used as an edible herb and traditional medicine for the treatment of inflammatory and infectious diseases. In the present study, we report the effects and molecular mechanism of the ethanolic extract of LF on cell proliferation, invasion and tube formation in human umbilical vein endothelial cells (HUVECs). LF-mediated inhibition of cell proliferation was accompanied by reduced expression of cell cycle-related proteins such as cyclin-dependent kinases (Cdks) and cyclins, leading to pRb hypophosphorylation and G1 phase cell cycle arrest. We also show that LF treatment inhibited cell invasion and tube formation in HUVECs. These anti-angiogenic activities of LF were associated with the inactivation of mitogenic signaling pathways, induction of vascular endothelial (VE)-cadherin distribution at cell-cell contacts and inhibition of matrix metalloproteinase (MMP) expression. Collectively, our findings demonstrate the pharmacological functions and molecular mechanisms of LF in regulating endothelial cell fates, and support further development as a potential therapeutic agent for the treatment and prevention of angiogenesis-related disorders including cancer.
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July-2015
Volume 34 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim JH, Kim H, Kim J, Ahn E, Ko H, Cho Y, Lee S, Bae G, Kim YK, Park JW, Park JW, et al: Ligularia fischeri inhibits endothelial cell proliferation, invasion and tube formation through the inactivation of mitogenic signaling pathways and regulation of vascular endothelial cadherin distribution and matrix metalloproteinase expression. Oncol Rep 34: 221-226, 2015
APA
Kim, J.H., Kim, H., Kim, J., Ahn, E., Ko, H., Cho, Y. ... Seo, D. (2015). Ligularia fischeri inhibits endothelial cell proliferation, invasion and tube formation through the inactivation of mitogenic signaling pathways and regulation of vascular endothelial cadherin distribution and matrix metalloproteinase expression. Oncology Reports, 34, 221-226. https://doi.org/10.3892/or.2015.4000
MLA
Kim, J. H., Kim, H., Kim, J., Ahn, E., Ko, H., Cho, Y., Lee, S., Bae, G., Kim, Y. K., Park, J. W., Oh, J. S., Seo, D."Ligularia fischeri inhibits endothelial cell proliferation, invasion and tube formation through the inactivation of mitogenic signaling pathways and regulation of vascular endothelial cadherin distribution and matrix metalloproteinase expression". Oncology Reports 34.1 (2015): 221-226.
Chicago
Kim, J. H., Kim, H., Kim, J., Ahn, E., Ko, H., Cho, Y., Lee, S., Bae, G., Kim, Y. K., Park, J. W., Oh, J. S., Seo, D."Ligularia fischeri inhibits endothelial cell proliferation, invasion and tube formation through the inactivation of mitogenic signaling pathways and regulation of vascular endothelial cadherin distribution and matrix metalloproteinase expression". Oncology Reports 34, no. 1 (2015): 221-226. https://doi.org/10.3892/or.2015.4000