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Article

Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures

  • Authors:
    • Lydia Rossow
    • Iris Eke
    • Ellen Dickreuter
    • Nils Cordes
  • View Affiliations / Copyright

    Affiliations: OncoRay-National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01307 Dresden, and Helmholtz-Zentrum Dresden-Rossendorf, D-01328 Dresden, Germany
  • Pages: 469-476
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    Published online on: May 25, 2015
       https://doi.org/10.3892/or.2015.4006
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Abstract

Epidermal growth factor receptor (EGFR) signaling plays an important role in tumor cell resistance to therapy. In addition to ligand binding, mutual and cooperative interactions of EGFR with integrin cell adhesion receptors critically influence proper downstream signaling through a number of bridging adapter proteins. In the present study, we analyzed the role of two of these adapter proteins, called PINCH1 and Nck2, for cellular radioresistance in combination with EGFR-targeting using the monoclonal antibody cetuximab. siRNA-mediated knockdown of PINCH1 or Nck2 resulted in enhanced radiosensitivity of 3D grown human squamous cell carcinoma cell lines FaDu (head and neck) and A431 (epidermis) comparable with effects seen after cetuximab treatment. Combination of knockdown and cetuximab did not result in additive nor synergistic effects regarding clonogenic radiation survival. Modifications in MAPK, Akt and FAK phosphorylation occurred upon cetuximab treatment as well as PINCH1 or Nck2 depletion. We further found this tumor cell radiosensitization to be due to attenuated repair of DNA double strand breaks and altered Rad50 and Nbs1 expression but without changes in other DNA repair proteins such as ATM, DNA-PK and Mre11. Our data suggest that the adaptor proteins PINCH1 and Nck2 critically contribute to cellular radioresistance and proper EGFR signaling in 3D lrECM grown human squamous cell carcinoma cells. Further investigations are warranted to identify the intracellular signaling network controlled by EGFR, PINCH1 and Nck2.
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1 

Mendelsohn J and Baselga J: Status of epidermal growth factor receptor antagonists in the biology and treatment of cancer. J Clin Oncol. 21:2787–2799. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Chong CR and Jänne PA: The quest to overcome resistance to EGFR-targeted therapies in cancer. Nat Med. 19:1389–1400. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Nyati MK, Morgan MA, Feng FY and Lawrence TS: Integration of EGFR inhibitors with radiochemotherapy. Nat Rev Cancer. 6:876–885. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Bonner JA, Harari PM, Giralt J, Cohen RB, Jones CU, Sur RK, Raben D, Baselga J, Spencer SA, Zhu J, et al: Radiotherapy plus cetuximab for locoregionally advanced head and neck cancer: 5-year survival data from a phase 3 randomised trial, and relation between cetuximab-induced rash and survival. Lancet Oncol. 11:21–28. 2010. View Article : Google Scholar

5 

Caudell JJ, Sawrie SM, Spencer SA, Desmond RA, Carroll WR, Peters GE, Nabell LM, Meredith RF and Bonner JA: Locoregionally advanced head and neck cancer treated with primary radiotherapy: A comparison of the addition of cetuximab or chemotherapy and the impact of protocol treatment. Int J Radiat Oncol Biol Phys. 71:676–681. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Yamada KM and Even-Ram S: Integrin regulation of growth factor receptors. Nat Cell Biol. 4:E75–E76. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Cabodi S, Moro L, Bergatto E, Boeri Erba E, Di Stefano P, Turco E, Tarone G and Defilippi P: Integrin regulation of epidermal growth factor (EGF) receptor and of EGF-dependent responses. Biochem Soc Trans. 32:438–442. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Eke I, Storch K, Krause M and Cordes N: Cetuximab attenuates its cytotoxic and radiosensitizing potential by inducing fibronectin biosynthesis. Cancer Res. 73:5869–5879. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Hehlgans S, Haase M and Cordes N: Signalling via integrins: Implications for cell survival and anticancer strategies. Biochim Biophys Acta. 1775:163–180. 2007.

10 

Braverman LE and Quilliam LA: Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck. J Biol Chem. 274:5542–5549. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Chiswell BP, Zhang R, Murphy JW, Boggon TJ and Calderwood DA: The structural basis of integrin-linked kinase-PINCH interactions. Proc Natl Acad Sci USA. 105:20677–20682. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Tu Y, Li F and Wu C: Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways. Mol Biol Cell. 9:3367–3382. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Vaynberg J, Fukuda T, Chen K, Vinogradova O, Velyvis A, Tu Y, Ng L, Wu C and Qin J: Structure of an ultraweak protein-protein complex and its crucial role in regulation of cell morphology and motility. Mol Cell. 17:513–523. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Legate KR, Montañez E, Kudlacek O and Fässler R: ILK, PINCH and parvin: The tIPP of integrin signalling. Nat Rev Mol Cell Biol. 7:20–31. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Xu Z, Fukuda T, Li Y, Zha X, Qin J and Wu C: Molecular dissection of PINCH-1 reveals a mechanism of coupling and uncoupling of cell shape modulation and survival. J Biol Chem. 280:27631–27637. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Eke I and Cordes N: Dual targeting of EGFR and focal adhesion kinase in 3D grown HNSCC cell cultures. Radiother Oncol. 99:279–286. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Eke I, Sandfort V, Storch K, Baumann M, Röper B and Cordes N: Pharmacological inhibition of EGFR tyrosine kinase affects ILK-mediated cellular radiosensitization in vitro. Int J Radiat Biol. 83:793–802. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Morello V, Cabodi S, Sigismund S, Camacho-Leal MP, Repetto D, Volante M, Papotti M, Turco E and Defilippi P: β1 integrin controls EGFR signaling and tumorigenic properties of lung cancer cells. Oncogene. 30:4087–4096. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Wang F, Weaver VM, Petersen OW, Larabell CA, Dedhar S, Briand P, Lupu R and Bissell MJ: Reciprocal interactions between beta1-integrin and epidermal growth factor receptor in three-dimensional basement membrane breast cultures: A different perspective in epithelial biology. Proc Natl Acad Sci USA. 95:14821–14826. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Eke I, Sandfort V, Mischkus A, Baumann M and Cordes N: Antiproliferative effects of EGFR tyrosine kinase inhibition and radiation-induced genotoxic injury are attenuated by adhesion to fibronectin. Radiother Oncol. 80:178–184. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Rodemann HP, Dittmann K and Toulany M: Radiation-induced EGFR-signaling and control of DNA-damage repair. Int J Radiat Biol. 83:781–791. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Huang S, Peet CR, Saker J, Li C, Armstrong EA, Kragh M, Pedersen MW and Harari PM: Sym004, a novel anti-EGFR antibody mixture, augments radiation response in human lung and head and neck cancers. Mol Cancer Ther. 12:2772–2781. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Eke I, Zscheppang K, Dickreuter E, Hickmann L, Mazzeo E, Unger K, Krause M and Cordes N: Simultaneous β1 integrin-EGFR targeting and radiosensitization of human head and neck cancer. J Natl Cancer Inst. 107:1072015. View Article : Google Scholar

24 

Eke I, Deuse Y, Hehlgans S, Gurtner K, Krause M, Baumann M, Shevchenko A, Sandfort V and Cordes N: β1Integrin/FAK/cortactin signaling is essential for human head and neck cancer resistance to radiotherapy. J Clin Invest. 122:1529–1540. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Storch K and Cordes N: Focal adhesion-chromatin linkage controls tumor cell resistance to radio- and chemotherapy. Chemother Res Pract. 2012:3192872012.PubMed/NCBI

26 

Eke I, Schneider L, Förster C, Zips D, Kunz-Schughart LA and Cordes N: EGFR/JIP-4/JNK2 signaling attenuates cetuximab-mediated radiosensitization of squamous cell carcinoma cells. Cancer Res. 73:297–306. 2013. View Article : Google Scholar

27 

Cordes N and Meineke V: Cell adhesion-mediated radioresistance (CAM-RR). Extracellular matrix-dependent improvement of cell survival in human tumor and normal cells in vitro. Strahlenther Onkol. 179:337–344. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Cordes N, Seidler J, Durzok R, Geinitz H and Brakebusch C: beta1-integrin-mediated signaling essentially contributes to cell survival after radiation-induced genotoxic injury. Oncogene. 25:1378–1390. 2006. View Article : Google Scholar

29 

Storch K, Eke I, Borgmann K, Krause M, Richter C, Becker K, Schröck E and Cordes N: Three-dimensional cell growth confers radioresistance by chromatin density modification. Cancer Res. 70:3925–3934. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Sandfort V, Eke I and Cordes N: The role of the focal adhesion protein PINCH1 for the radiosensitivity of adhesion and suspension cell cultures. PLoS One. 5:e130562010. View Article : Google Scholar : PubMed/NCBI

31 

Estrugo D, Fischer A, Hess F, Scherthan H, Belka C and Cordes N: Ligand bound beta1 integrins inhibit procaspase-8 for mediating cell adhesion-mediated drug and radiation resistance in human leukemia cells. PLoS One. 2:e2692007. View Article : Google Scholar : PubMed/NCBI

32 

Ito S, Takahara Y, Hyodo T, Hasegawa H, Asano E, Hamaguchi M and Senga T: The roles of two distinct regions of PINCH-1 in the regulation of cell attachment and spreading. Mol Biol Cell. 21:4120–4129. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Fukuda T, Chen K, Shi X and Wu C: PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival. J Biol Chem. 278:51324–51333. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Eke I, Koch U, Hehlgans S, Sandfort V, Stanchi F, Zips D, Baumann M, Shevchenko A, Pilarsky C, Haase M, et al: PINCH1 regulates Akt1 activation and enhances radioresistance by inhibiting PP1alpha. J Clin Invest. 120:2516–2527. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Funasaka K, Ito S, Hasegawa H, Goldberg GS, Hirooka Y, Goto H, Hamaguchi M and Senga T: Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing. FEBS J. 277:3502–3513. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Labelle-Côté M, Dusseault J, Ismaïl S, Picard-Cloutier A, Siegel PM and Larose L: Nck2 promotes human melanoma cell proliferation, migration and invasion in vitro and primary melanoma-derived tumor growth in vivo. BMC Cancer. 11:4432011. View Article : Google Scholar : PubMed/NCBI

37 

Errington TM and Macara IG: Depletion of the adaptor protein NCK increases uV-induced p53 phosphorylation and promotes apoptosis. PLoS One. 8:e762042013. View Article : Google Scholar : PubMed/NCBI

38 

Chen K, Tu Y, Zhang Y, Blair HC, Zhang L and Wu C: PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells. J Biol Chem. 283:2508–2517. 2008. View Article : Google Scholar

39 

Gonzalez-Nieves R, Desantis AI and Cutler ML: Rsu1 contributes to regulation of cell adhesion and spreading by PINCH1-dependent and -independent mechanisms. J Cell Commun Signal. 7:279–293. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Stanchi F, Grashoff C, Nguemeni Yonga CF, Grall D, Fässler R and Van Obberghen-Schilling E: Molecular dissection of the ILK-PINCH-parvin triad reveals a fundamental role for the ILK kinase domain in the late stages of focal-adhesion maturation. J Cell Sci. 122:1800–1811. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Montanez E, Karaköse E, Tischner D, Villunger A and Fässler R: PINCH-1 promotes Bcl-2-dependent survival signalling and inhibits JNK-mediated apoptosis in the primitive endoderm. J Cell Sci. 125:5233–5240. 2012. View Article : Google Scholar : PubMed/NCBI

42 

Rea K, Sensi M, Anichini A, Canevari S and Tomassetti A: EGFR/MEK/ERK/CDK5-dependent integrin-independent FAK phosphorylated on serine 732 contributes to microtubule depolymerization and mitosis in tumor cells. Cell Death Dis. 4:e8152013. View Article : Google Scholar : PubMed/NCBI

43 

Wang D, Li Y, Wu C and Liu Y: PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression. PLoS One. 6:e170482011. View Article : Google Scholar : PubMed/NCBI

44 

Kremer BE, Adang LA and Macara IG: Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell. 130:837–850. 2007. View Article : Google Scholar : PubMed/NCBI

45 

Jahn T, Seipel P, Coutinho S, Miething C, Peschel C and Duyster J: Grb4/Nckbeta acts as a nuclear repressor of v-Abl-induced transcription from c-jun/c-fos promoter elements. J Biol Chem. 276:43419–43427. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Rossow L, Eke I, Dickreuter E and Cordes N: Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures. Oncol Rep 34: 469-476, 2015.
APA
Rossow, L., Eke, I., Dickreuter, E., & Cordes, N. (2015). Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures. Oncology Reports, 34, 469-476. https://doi.org/10.3892/or.2015.4006
MLA
Rossow, L., Eke, I., Dickreuter, E., Cordes, N."Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures". Oncology Reports 34.1 (2015): 469-476.
Chicago
Rossow, L., Eke, I., Dickreuter, E., Cordes, N."Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures". Oncology Reports 34, no. 1 (2015): 469-476. https://doi.org/10.3892/or.2015.4006
Copy and paste a formatted citation
x
Spandidos Publications style
Rossow L, Eke I, Dickreuter E and Cordes N: Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures. Oncol Rep 34: 469-476, 2015.
APA
Rossow, L., Eke, I., Dickreuter, E., & Cordes, N. (2015). Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures. Oncology Reports, 34, 469-476. https://doi.org/10.3892/or.2015.4006
MLA
Rossow, L., Eke, I., Dickreuter, E., Cordes, N."Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures". Oncology Reports 34.1 (2015): 469-476.
Chicago
Rossow, L., Eke, I., Dickreuter, E., Cordes, N."Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures". Oncology Reports 34, no. 1 (2015): 469-476. https://doi.org/10.3892/or.2015.4006
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