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Article

Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin

  • Authors:
    • Zhou-Jing Ji
    • Jian-Li Wang
    • Li Chen
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology and Venereology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China, Department of Pathological Anatomy, Nantong University, Nantong, Jiangsu, P.R. China
  • Pages: 811-822
    |
    Published online on: June 15, 2015
       https://doi.org/10.3892/or.2015.4062
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Abstract

The simultaneous silencing of multiple upregulated genes is an attractive and viable strategy to treat many incurable diseases including cancer. In the present study, skin squamous cell carcinoma (SSCC) tissue microarray was constructed and the expression of NET-1 and survivin was identified. The high expression of NET-1 and survivin gene in SSCC was confirmed as an important event for the formation and development of the cancer. A total of 100 primary SSCC patients were included in the present study. Expression of NET-1 and survivin in cancer cells was evaluated immunohistochemically in tissue microarrays. The interaction between NET-1 and survivin in SSCC by co-immunoprecipitation was subsequently verified by producing the siRNA sequence targeting the single gene (siRNA-NET-1 and siRNA-survivin) as well as NET-1 and survivin gene (one-chain-double-target siRNA). The levels of NET-1 and survivin mRNA and protein expression in A431 cells were detected by RT-qPCR and western blotting, and the expression of related genes including vascular endothelial growth factor (VEGF), cortactin, Bcl-2, caspase-3 and -8 was identified using RT-qPCR. The protein localization and expression of NET-1 and survivin in A431 cells were documented by immunohistochemistry and immuno­fluorescence staining. The proliferation and apoptosis of A431 cells were detected by CCK-8 assay and flow cytometry (FCM). The tissue microarray showed that NET-1 and survivin were highly expressed in SSCC, while the correlation analysis showed NET-1 expression was positively associated with survivin. In addition, we reported that using the one‑chain-double-target siRNA conjugate composed of NET-1 and survivin siRNA sequences in the same backbone inhibited proliferation and promoted apoptosis of SSCC. The one‑chain-double-target siRNA showed further downregulation on NET-1 and survivin mRNA and protein levels compared with NET-1 siRNA or survivin siRNA. It also exhibited greater suppression on proliferation and triggering of apoptosis in A431 cells than NET-1 siRNA or survivin siRNA. This result may be explained by the significant downregulation of VEGF, cortactin and Bcl-2, and upregulation of caspase-3 and -8. NET-1 and survivin were overexpressed in SSCC and an interaction between NET-1 and survivin was identified. The one-chain-double-target siRNA appears to be superior in inhibiting cell proliferation and promoting apoptosis compared with the single target siRNA. NET-1 and survivin may have correlative signaling pathways with VEGF, cortactin, Bcl-2, caspase-3 and -8. Simultaneous silencing of NET-1 and survivin using one-chain-double-target siRNA thus provides an advantageous alternative in the development of therapeutics for SSCC.
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View References

1 

Smoller BR: Squamous cell carcinoma: From precursor lesions to high-risk variants. Mod Pathol. 19(Suppl 2): S88–S92. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Bowen GM, White GL Jr and Gerwels JW: Mohs micrographic surgery. Am Fam Physician. 72:845–848. 2005.PubMed/NCBI

3 

Serru V, Dessen P, Boucheix C and Rubinstein E: Sequence and expression of seven new tetraspans. Biochim Biophys Acta. 1478:159–163. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Maecker HT, Todd SC and Levy S: The tetraspanin superfamily: Molecular facilitators. FASEB J. 11:428–442. 1997.PubMed/NCBI

5 

Yauch RL and Hemler ME: Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase. Biochem J. 351:629–637. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Chen L, Wang JL, Li H, Qin J and Wu YY: Functions of NET-1 gene in skin squamous cell carcinoma cell line (A431): A siRNA study. Zhonghua Bing Li Xue Za Zhi. 38:691–696. 2009.In Chinese.

7 

Chen L, Li X, Wang GL, Wang Y, Zhu YY and Zhu J: Clinicopathological significance of overexpression of TSPAN1, Ki67 and CD34 in gastric carcinoma. Tumori. 94:531–538. 2008.PubMed/NCBI

8 

Wollscheid V, Kühne-Heid R, Stein I, Jansen L, Köllner S, Schneider A and Dürst M: Identification of a new proliferation-associated protein NET-1/C4.8 characteristic for a subset of high-grade cervical intraepithelial neoplasia and cervical carcinomas. Int J Cancer. 99:771–775. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Chen L, Zhu YY, Zhang XJ, Wang GL, Li XY, He S, Zhang JB and Zhu JW: TSPAN1 protein expression: A significant prognostic indicator for patients with colorectal adenocarcinoma. World J Gastroenterol. 15:2270–2276. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Scholz CJ, Kurzeder C, Koretz K, Windisch J, Kreienberg R, Sauer G and Deissler H: Tspan-1 is a tetraspanin preferentially expressed by mucinous and endometrioid subtypes of human ovarian carcinomas. Cancer Lett. 275:198–203. 2009. View Article : Google Scholar

11 

Chen L, Zhu Y, Li H, Wang GL, Wu YY, Lu YX, Qin J, Tuo J, Wang JL and Zhu J: Knockdown of TSPAN1 by RNA silencing and antisense technique inhibits proliferation and infiltration of human skin squamous carcinoma cells. Tumori. 96:289–295. 2010.PubMed/NCBI

12 

Kim GY, Lim SJ and Kim YW: Expression of HuR, COX-2, and survivin in lung cancers; cytoplasmic HuR stabilizes cyclooxy-genase-2 in squamous cell carcinomas. Mod Pathol. 24:1336–1347. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Ryan BM, O’Donovan N and Duffy MJ: Survivin: A new target for anti-cancer therapy. Cancer Treat Rev. 35:553–562. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Cheng K and Mahato RI: Gene modulation for treating liver fibrosis. Crit Rev Ther Drug Carrier Syst. 24:93–146. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Mahato RI, Cheng K and Guntaka RV: Modulation of gene expression by antisense and antigene oligodeoxynucleotides and small interfering RNA. Expert Opin Drug Deliv. 2:3–28. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Chen L, Wang Z, Zhan X, Li DC, Zhu YY and Zhu J: Association of NET-1 gene expression with human hepatocellular carcinoma. Int J Surg Pathol. 15:346–353. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Zaffaroni N, Pennati M and Daidone MG: Survivin as a target for new anticancer interventions. J Cell Mol Med. 9:360–372. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Wu JB, Fu HQ, Huang LZ, Liu AW and Zhang JX: Effects of siRNA-targeting BMP-2 on the abilities of migration and invasion of human liver cancer SMMC7721 cells and its mechanism. Cancer Gene Ther. 18:20–25. 2011. View Article : Google Scholar

19 

Chen SM, Wang Y, Xiao BK and Tao ZZ: Effect of blocking VEGF, hTERT and Bcl-xl by multiple shRNA expression vectors on the human laryngeal squamous carcinoma xenograft in nude mice. Cancer Biol Ther. 7:734–739. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Takahashi H and Shibuya M: The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clin Sci. 109:227–241. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Yamada S, Yanamoto S, Kawasaki G, Mizuno A and Nemoto TK: Overexpression of cortactin increases invasion potential in oral squamous cell carcinoma. Pathol Oncol Res. 16:523–531. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Hsu KF, Lin CK, Yu CP, Tzao C, Lee SC, Lee YY, Tsai WC and Jin JS: Cortactin, fascin, and survivin expression associated with clinicopathological parameters in esophageal squamous cell carcinoma. Dis Esophagus. 22:402–408. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Thomas S, Quinn BA, Das SK, Dash R, Emdad L, Dasgupta S, Wang XY, Dent P, Reed JC, Pellecchia M, et al: Targeting the Bcl-2 family for cancer therapy. Expert Opin Ther Targets. 17:61–75. 2013. View Article : Google Scholar

24 

Ghavami S, Hashemi M, Ande SR, Yeganeh B, Xiao W, Eshraghi M, Bus CJ, Kadkhoda K, Wiechec E, Halayko AJ, et al: Apoptosis and cancer: Mutations within caspase genes. J Med Genet. 46:497–510. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Jeong SY and Seol DW: The role of mitochondria in apoptosis. BMB Rep. 41:11–22. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ji Z, Wang J and Chen L: Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin. Oncol Rep 34: 811-822, 2015.
APA
Ji, Z., Wang, J., & Chen, L. (2015). Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin. Oncology Reports, 34, 811-822. https://doi.org/10.3892/or.2015.4062
MLA
Ji, Z., Wang, J., Chen, L."Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin". Oncology Reports 34.2 (2015): 811-822.
Chicago
Ji, Z., Wang, J., Chen, L."Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin". Oncology Reports 34, no. 2 (2015): 811-822. https://doi.org/10.3892/or.2015.4062
Copy and paste a formatted citation
x
Spandidos Publications style
Ji Z, Wang J and Chen L: Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin. Oncol Rep 34: 811-822, 2015.
APA
Ji, Z., Wang, J., & Chen, L. (2015). Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin. Oncology Reports, 34, 811-822. https://doi.org/10.3892/or.2015.4062
MLA
Ji, Z., Wang, J., Chen, L."Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin". Oncology Reports 34.2 (2015): 811-822.
Chicago
Ji, Z., Wang, J., Chen, L."Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin". Oncology Reports 34, no. 2 (2015): 811-822. https://doi.org/10.3892/or.2015.4062
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