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August-2015 Volume 34 Issue 2

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo

  • Authors:
    • Ka Woon Karen Chan
    • Wing Shing Ho
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, P.R. China
  • Pages: 673-680
    |
    Published online on: June 16, 2015
       https://doi.org/10.3892/or.2015.4068
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Abstract

Accumulation of an excess amount of reactive oxygen species (ROS) can cause hepatotoxicity that may result in liver damage. Therefore, development of anti-oxidative agents is needed for reducing liver toxicity. This study investigated the anti-oxidative and hepatoprotective activity of lithospermic acid, a plant-derived polycyclic phenolic carboxylic acid isolated from Salvia miltiorrhiza, on carbon tetrachloride (CCl4)-induced acute liver damage in vitro and in vivo. The results of the DPPH assay indicated that lithospermic acid was a good anti-oxidant. the CCl4-exposed Huh7 cell line exhibited decreased cell viability, increased necrosis and elevated ROS and caspase-3/7 activity. Lithospermic acid significantly attenuated the CCl4-induced oxidative damage in a concentration-dependent manner. The result of an in vivo study with BALB/c mice corresponded with the anti-oxidative activity noted in the in vitro study. Exposure of mice to CCl4 resulted in a greater than 2-fold elevation in serum aspartate transaminase (AST) and alanine transaminase (ALT). levels In addition, CCl4-intoxication led to an over 20% decrease in the level of intracellular hepatic enzymes including superoxide dismutase (SOD) and catalase (CAT) as well as increased lipid peroxidation. Upon histological examination of the CCl4-exposed mice, the mouse livers showed severe hepatic damage with a huge section of necrosis and structural destruction. Pretreatment of mice with lithospermic acid for six days significantly reduced CCl4-induced hepatic oxidative damage, serum AST and ALT. The pretreatment also increased SOD and CAT. The findings suggest that the health status of the liver was improved comparable to the control group after a high-dose treatment with lithospermic acid (100 mg/kg weight). The potential applicability of lithospermic acid as a hepatoprotective agent was demonstrated.
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View References

1 

Parsons CJ, Takashima M and Rippe RA: Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol. 22(Suppl 1): S79–S84. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Zhang C, Lu X, Tan Y, Li B, Miao X, Jin L, Shi X, Zhang X, Miao L, Li X and Cai L: Diabetes-induced hepatic pathogenic damage, inflammation, oxidative stress, and insulin resistance was exacerbated in zinc deficient mouse model. PLoS One. 7:e492572012. View Article : Google Scholar : PubMed/NCBI

3 

Johnston DE and Kroening C: Mechanism of early carbon tetrachloride toxicity in cultured rat hepatocytes. Pharmacol Toxicol. 83:231–239. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Vuda M, D’Souza R, Upadhya S, Kumar V, Rao N, Kumar V, Boillat C and Mungli P: Hepatoprotective and antioxidant activity of aqueous extract of Hybanthus enneaspermus against CCl4-induced liver injury in rats. Exp Toxicol Pathol. 64:855–859. 2012. View Article : Google Scholar

5 

Wang R, Yu XY, Guo ZY, Wang YJ, Wu Y and Yuan YF: Inhibitory effects of salvianolic acid B on CCl(4)-induced hepatic fibrosis through regulating NF-κB/IκBα signaling. J Ethnopharmacol. 144:592–598. 2012. View Article : Google Scholar : PubMed/NCBI

6 

She GM, Xu C, Liu B and Shi RB: Polyphenolic acids from mint (the aerial of Mentha haplocalyx Briq.) with DPPH radical scavenging activity. J Food Sci. 75:C359–C362. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Lattanzio V, Cardinali A, Ruta C, Fortunato IM, Lattanzio VMT, Linsalata V and Cicco N: Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress. Environ Exp Bot. 65:54–62. 2009. View Article : Google Scholar

8 

Liu X, Chen R, Shang Y, Jiao B and Huang C: Lithospermic acid as a novel xanthine oxidase inhibitor has anti-inflammatory and hypouricemic effects in rats. Chem Biol Interact. 176:137–142. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Matkowski A: Plant in vitro culture for the production of antioxidants: a review. Biotechnol Adv. 26:548–560. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Kang DG, Oh H, Sohn EJ, Hur TY, Lee KC, Kim KJ, Kim TY and Lee HS: Lithospermic acid B isolated from Salvia miltiorrhiza ameliorates ischemia/reperfusion-induced renal injury in rats. Life Sci. 75:1801–1816. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Li X, Yu C, Lu Y, Gu Y, Lu J, Xu W, Xuan L and Wang Y: Pharmacokinetics, tissue distribution, metabolism, and excretion of depside salts from Salvia miltiorrhiza in rats. Drug Metab Dispos. 35:234–239. 2007. View Article : Google Scholar

12 

Zhang Y, Akao T, Nakamura N, Hattori M, Yang XW, Duan CL and Liu JX: Magnesium lithospermate B is excreted rapidly into rat bile mostly as methylated metabolites, which are potent antioxidants. Drug Metab Dispos. 32:752–757. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Chen L, Wang WY and Wang YP: Inhibitory effects of litho-spermic acid on proliferation and migration of rat vascular smooth muscle cells. Acta Pharmacol Sin. 30:1245–1252. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Jie M, Cheung WM, Yu V, Zhou Y, Tong PH and Ho JW: Anti-proliferative activities of sinigrin on carcinogen-induced hepatotoxicity in rats. PLoS One. 9:e1101452014. View Article : Google Scholar : PubMed/NCBI

15 

Brito NJ, López JA, do Nascimento MA, Macêdo JB, Silva GA, Oliveira CN, de Rezende AA, Brandão-Neto J, Schwarz A and Almeida M: Antioxidant activity and protective effect of Turnera ulmifolia Linn. var elegans against carbon tetrachloride-induced oxidative damage in rats. Food Chem Toxicol. 50:4340–4347. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Brand-Williams W, Cuvelier ME and Berset C: Use of a free radical method to evaluate antioxidant activity. Food Sci Technol (Campinas). 28:25–30. 1995.

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Copy and paste a formatted citation
Spandidos Publications style
Chan KW and Ho WS: Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo. Oncol Rep 34: 673-680, 2015.
APA
Chan, K.W., & Ho, W.S. (2015). Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo. Oncology Reports, 34, 673-680. https://doi.org/10.3892/or.2015.4068
MLA
Chan, K. W., Ho, W. S."Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo". Oncology Reports 34.2 (2015): 673-680.
Chicago
Chan, K. W., Ho, W. S."Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo". Oncology Reports 34, no. 2 (2015): 673-680. https://doi.org/10.3892/or.2015.4068
Copy and paste a formatted citation
x
Spandidos Publications style
Chan KW and Ho WS: Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo. Oncol Rep 34: 673-680, 2015.
APA
Chan, K.W., & Ho, W.S. (2015). Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo. Oncology Reports, 34, 673-680. https://doi.org/10.3892/or.2015.4068
MLA
Chan, K. W., Ho, W. S."Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo". Oncology Reports 34.2 (2015): 673-680.
Chicago
Chan, K. W., Ho, W. S."Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo". Oncology Reports 34, no. 2 (2015): 673-680. https://doi.org/10.3892/or.2015.4068
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