PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway

Xu,Jinye; Cai,Junbo; Jin,Xiaoyan; Yang,Jingjin; Shen,Qinyan; Ding,Xiaofei; Liang,Yong

doi:10.3892/or.2015.4096

PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway

Authors:
- Jinye Xu
- Junbo Cai
- Xiaoyan Jin
- Jingjin Yang
- Qinyan Shen
- Xiaofei Ding
- Yong Liang
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Affiliations: Department of Oncologic Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Oncologic Surgery, The Affiliated Municipal Hospital, Taizhou University, Taizhou, Zhejiang 318000, P.R. China, Department of Clinical Laboratory, Medical School, Taizhou University, Taizhou, Zhejiang 318000, P.R. China
Published online on: June 30, 2015 https://doi.org/10.3892/or.2015.4096
Pages: 1424-1430

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Abstract

The p53-inducible gene 3 (PIG3 or TP53I3) is a downstream gene of p53, which can be involved in the process of apoptosis induced by p53 via the production of reactive oxygen species (ROS). However, the functional significance of PIG3 in cancer remains to be determined. This aim of this study was to examine the mRNA and protein expression of PIG3 in papillary thyroid carcinoma (PTC) and normal thyroid tissues, assess the relationship between PIG3 expression and clinicopathological parameters in PTC and examine its role in the proliferation of PTC cell lines. The results showed that PIG3 was aberrantly overexpressed in the majority of specimens of PTC while the expression of p53 was lower in PTC compared with normal thyroid tissues. Anti-PIG3 immunoreactivity positively correlated with TNM grade. In the PTC cell lines, PIG3 silencing using small interfering RNA (siRNAs) impaired their ability of proliferation and decreased the activity of the PI3K/AKT/PTEN pathway. The results suggested that PIG3 plays an oncogenic role in PTC via the regulation of the PI3K/AKT/PTEN pathway and support the exploration of PIG3 as a novel biomarker for patients with PTC.

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