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Article Open Access

miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3

  • Authors:
    • Hong Jiang
    • Wei-Wei Yu
    • Lu-Lu Wang
    • Yang Peng
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1153-1161
    |
    Published online on: July 1, 2015
       https://doi.org/10.3892/or.2015.4099
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Abstract

MicroRNAs (miRNAs) are abnormally expressed in various types of cancer. miR-130a expression and function in gastric cancer has yet to be elucidated. The aim of the present study was to identify the miR-130a expression and function in gastric cancer. miR-130a expression was examined in gastric cancer cell lines and tissues by RT-qPCR. The diagnostic and prognostic significance of miR-130a in gastric cancer was analyzed by receiver-operating characteristic (ROC) curve and Kaplan-Meier analysis. miR130a expression was identified and the diagnostic significance in the serum of gastric cancer patients and healthy controls was analyzed using RT-qPCR and ROC curves, respectively. A target gene for miR-130a was identified using luciferase reporter assays, and gastric cancer tumorigenesis ability was examined by 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. The results showed that miR‑130a was upregulated in gastric cancer. The low-miR-130a group had significantly improved overall survival compared to the high-miR-130a group. Furthermore, the expression of miR‑130a in plasma in gastric cancer patients was upregulated and diagnostic value for gastric cancer of miR-130a is more effective than the tumor markers carcinoembryonic antigen (CEA) and CA-199. miR-130a directly targeted runt‑related transcription factor 3 (RUNX3) and promoted gastric cancer tumorigenesis by targeting RUNX3. miR-130a may therefore be a useful marker for the diagnosis and prognosis of gastric cancer. Additionally, miR-130a was identified as an oncogene that promotes gastric cancer tumorigenesis by targeting RUNX3.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Yu W, Wang L and Peng Y: miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3. Oncol Rep 34: 1153-1161, 2015.
APA
Jiang, H., Yu, W., Wang, L., & Peng, Y. (2015). miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3. Oncology Reports, 34, 1153-1161. https://doi.org/10.3892/or.2015.4099
MLA
Jiang, H., Yu, W., Wang, L., Peng, Y."miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3". Oncology Reports 34.3 (2015): 1153-1161.
Chicago
Jiang, H., Yu, W., Wang, L., Peng, Y."miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3". Oncology Reports 34, no. 3 (2015): 1153-1161. https://doi.org/10.3892/or.2015.4099
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Yu W, Wang L and Peng Y: miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3. Oncol Rep 34: 1153-1161, 2015.
APA
Jiang, H., Yu, W., Wang, L., & Peng, Y. (2015). miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3. Oncology Reports, 34, 1153-1161. https://doi.org/10.3892/or.2015.4099
MLA
Jiang, H., Yu, W., Wang, L., Peng, Y."miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3". Oncology Reports 34.3 (2015): 1153-1161.
Chicago
Jiang, H., Yu, W., Wang, L., Peng, Y."miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3". Oncology Reports 34, no. 3 (2015): 1153-1161. https://doi.org/10.3892/or.2015.4099
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