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Article

miR-133a inhibits cervical cancer growth by targeting EGFR

Retraction in: /10.3892/or.2021.8103
  • Authors:
    • Xuesong Song
    • Bo Shi
    • Kexin Huang
    • Wenjie Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Hospital of Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China, The Experiment Center, College of Basic Medical Sciences, Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China, Department of Pathology, China‑Japan Union Hospital of Jilin University, Nanguan, Changchun, Jilin 130033, P.R. China
  • Pages: 1573-1580
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/or.2015.4101
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in cervical carcinogenesis and progression. microRNA-133a (miR-133a) has been reported to play a tumor-suppressor role in a range of cancers. However, the role and underlying molecular mechanism of miR-133a in cervical cancer have not been investigated. In the present study, we investigated the role of miR-133a in the tumorigenicity of cervical cancer cells in vivo and in vitro. The expression of miR-133a was investigated using real-time reverse transcription-polymerase chain reaction (qRT-PCR) in 30 cervical specimens and matched adjacent normal tissues and cervical cancer cell lines. We found that the expression level of miR‑133a was significantly downregulated in cervical cancer tissues and cervical cancer cell lines, and the aberrant expression of miR-133a was correlated with lymph node metastasis, histological grade and FIGO stage. The role of miR-133a in tumorigenicity of cervical cancer cells was assessed by the restoration of miR-133a. We found that restoration of miR‑133a inhibited cell proliferation, colony formation, migration and invasion, promoted cell apoptosis in vitro and suppressed tumorigenicity in vivo. The epidermal growth factor receptor (EGFR) was confirmed to be a direct target of miR-133a in cervical cancer cells using luciferase assay and western blotting. Restoration of miR-133a inhibited EGFR expression and activated the AKT and ERK signaling pathways. These results showed that miR-133a suppresses cervical cancer growth in vitro and in vivo through targeting EGFR, suggesting that miR-133a can be a potential target for the treatment of cervical cancer.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Martin-Hirsch PL and Wood NJ: Cervical cancer. BMJ Clin Evid. 2011:08182011.PubMed/NCBI

3 

Wei LH: Prevention and treatment of cervical cancer, it is a long-term and arduous task. Zhonghua Fu Chan Ke Za Zhi. 48:304–306. 2013.In Chinese. PubMed/NCBI

4 

Chaturvedi AK: Beyond cervical cancer: Burden of other HPV-related cancers among men and women. J Adolesc Health. 46(Suppl 4): S20–26. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Hildesheim A and Wang SS: Host and viral genetics and risk of cervical cancer: A review. Virus Res. 89:229–240. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Fabian MR, Sonenberg N and Filipowicz W: Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem. 79:351–379. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Guo H, Ingolia NT, Weissman JS and Bartel DP: Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature. 466:835–840. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Farazi TA, Spitzer JI, Morozov P and Tuschl T: miRNAs in human cancer. J Pathol. 223:102–115. 2011. View Article : Google Scholar :

10 

McManus MT: MicroRNAs and cancer. Semin Cancer Biol. 13:253–258. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W and Tuschl T: Identification of tissue-specific microRNAs from mouse. Curr Biol. 12:735–739. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Feng Y, Niu LL, Wei W, Zhang WY, Li XY, Cao JH and Zhao SH: A feedback circuit between miR-133 and the ERK1/2 pathway involving an exquisite mechanism for regulating myoblast proliferation and differentiation. Cell Death Dis. 4:e9342013. View Article : Google Scholar : PubMed/NCBI

13 

Kojima S, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nohata N, Fuse M, Ichikawa T, Naya Y, Nakagawa M, et al: Tumour suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer. Br J Cancer. 106:405–413. 2012. View Article : Google Scholar :

14 

Nohata N, Hanazawa T, Kikkawa N, Mutallip M, Fujimura L, Yoshino H, Kawakami K, Chiyomaru T, Enokida H, Nakagawa M, et al: Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma. Int J Oncol. 38:209–217. 2011.

15 

Cui W, Zhang S, Shan C, Zhou L and Zhou Z: microRNA-133a regulates the cell cycle and proliferation of breast cancer cells by targeting epidermal growth factor receptor through the EGFR/Akt signaling pathway. FEBS J. 280:3962–3974. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, Nishiyama K, Fujimura L, Kikkawa N, Seki N and Nakagawa M: miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer. Br J Cancer. 102:883–891. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Kano M, Seki N, Kikkawa N, Fujimura L, Hoshino I, Akutsu Y, Chiyomaru T, Enokida H, Nakagawa M and Matsubara H: miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int J Cancer. 127:2804–2814. 2010. View Article : Google Scholar

18 

Dong Y, Zhao J, Wu CW, Zhang L, Liu X, Kang W, Leung WW, Zhang N, Chan FK, Sung JJ, et al: Tumor suppressor functions of miR-133a in colorectal cancer. Mol Cancer Res. 11:1051–1060. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Nicholson RI, Gee JM and Harper ME: EGFR and cancer prognosis. Eur J Cancer. 37(Suppl 4): S9–S15. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Zhang W, Jiang Y, Yu Q, Qiang S, Liang P, Gao Y, Zhao X, Liu W and Zhang J: EGFR promoter methylation, EGFR mutation, and HPV infection in chinese cervical squamous cell carcinoma. Appl Immunohistochem Mol Morphol. Mar 16–2015.Epub ahead of print. View Article : Google Scholar

21 

Herbst RS: Review of epidermal growth factor receptor biology. Int J Radiat Oncol Biol Phys. 59(Suppl 2): S21–S26. 2004. View Article : Google Scholar

22 

Oda K, Matsuoka Y, Funahashi A and Kitano H: A comprehensive pathway map of epidermal growth factor receptor signaling. Mol Syst Biol. 1:2005.00102005. View Article : Google Scholar

23 

Schickel R, Boyerinas B, Park SM and Peter ME: MicroRNAs: Key players in the immune system, differentiation, tumorigenesis and cell death. Oncogene. 27:5959–5974. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Banno K, Iida M, Yanokura M, Kisu I, Iwata T, Tominaga E, Tanaka K and Aoki D: MicroRNA in cervical cancer: OncomiRs and tumor suppressor miRs in diagnosis and treatment. Sci World J. 2014:1780752014. View Article : Google Scholar

25 

Ji F, Zhang H, Wang Y, Li M, Xu W, Kang Y, Wang Z, Wang Z, Cheng P, Tong D, et al: MicroRNA-133a, downregulated in osteosarcoma, suppresses proliferation and promotes apoptosis by targeting Bcl-xL and Mcl-1. Bone. 56:220–226. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Moriya Y, Nohata N, Kinoshita T, Mutallip M, Okamoto T, Yoshida S, Suzuki M, Yoshino I and Seki N: Tumor suppressive microRNA-133a regulates novel molecular networks in lung squamous cell carcinoma. J Hum Genet. 57:38–45. 2012. View Article : Google Scholar

27 

Siciliano V, Garzilli I, Fracassi C, Criscuolo S, Ventre S and di Bernardo D: MiRNAs confer phenotypic robustness to gene networks by suppressing biological noise. Nat Commun. 4:23642013. View Article : Google Scholar : PubMed/NCBI

28 

Wang LK, Hsiao TH, Hong TM, Chen HY, Kao SH, Wang WL, Yu SL, Lin CW and Yang PC: MicroRNA-133a suppresses multiple oncogenic membrane receptors and cell invasion in non-small cell lung carcinoma. PLoS One. 9:e967652014. View Article : Google Scholar : PubMed/NCBI

29 

Tao J, Wu D, Xu B, Qian W, Li P, Lu Q, Yin C and Zhang W: microRNA-133 inhibits cell proliferation, migration and invasion in prostate cancer cells by targeting the epidermal growth factor receptor. Oncol Rep. 27:1967–1975. 2012.PubMed/NCBI

30 

Fukazawa EM, Baiocchi G, Soares FA, Kumagai LY, Faloppa CC, Badiglian-Filho L, Coelho FR, Gonçalves WJ, Costa RL and Góes JC: Cox-2, EGFR, and ERBB-2 expression in cervical intraepithelial neoplasia and cervical cancer using an automated imaging system. Int J Gynecol Pathol. 33:225–234. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Carcereny E, Morán T, Capdevila L, Cros S, Vilà L, de Los Llanos Gil M, Remón J and Rosell R: The epidermal growth factor receptor (EGRF) in lung cancer. Transl Respir Med. 3:12015. View Article : Google Scholar : PubMed/NCBI

32 

Xu H, Yu Y, Marciniak D, Rishi AK, Sarkar FH, Kucuk O and Majumdar AP: Epidermal growth factor receptor (EGFR)-related protein inhibits multiple members of the EGFR family in colon and breast cancer cells. Mol Cancer Ther. 4:435–442. 2005.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Song X, Shi B, Huang K and Zhang W: miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103. Oncol Rep 34: 1573-1580, 2015.
APA
Song, X., Shi, B., Huang, K., & Zhang, W. (2015). miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103. Oncology Reports, 34, 1573-1580. https://doi.org/10.3892/or.2015.4101
MLA
Song, X., Shi, B., Huang, K., Zhang, W."miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103". Oncology Reports 34.3 (2015): 1573-1580.
Chicago
Song, X., Shi, B., Huang, K., Zhang, W."miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103". Oncology Reports 34, no. 3 (2015): 1573-1580. https://doi.org/10.3892/or.2015.4101
Copy and paste a formatted citation
x
Spandidos Publications style
Song X, Shi B, Huang K and Zhang W: miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103. Oncol Rep 34: 1573-1580, 2015.
APA
Song, X., Shi, B., Huang, K., & Zhang, W. (2015). miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103. Oncology Reports, 34, 1573-1580. https://doi.org/10.3892/or.2015.4101
MLA
Song, X., Shi, B., Huang, K., Zhang, W."miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103". Oncology Reports 34.3 (2015): 1573-1580.
Chicago
Song, X., Shi, B., Huang, K., Zhang, W."miR-133a inhibits cervical cancer growth by targeting EGFR Retraction in /10.3892/or.2021.8103". Oncology Reports 34, no. 3 (2015): 1573-1580. https://doi.org/10.3892/or.2015.4101
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