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Article

Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria

  • Authors:
    • Haiyang Jiang
    • Lei Zhao
    • Xiaoshen Dong
    • Anning He
    • Caiwei Zheng
    • Magnus Johansson
    • Anna Karlsson
    • Xinyu Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning, P.R. China, Center of Experiment Technology and Medical Research, China Medical University, Shenyang, Liaoning, P.R. China, Laboratory 1, Cancer Institute, China Medical University, Shenyang, Liaoning, P.R. China, Brandeis University, Waltham, MA, USA, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden
  • Pages: 1487-1493
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/or.2015.4102
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Abstract

Heterologous expression of the Drosophila melanogaster multi-substrate deoxyribonucleoside kinase (Dm-dNK) increases the sensitivity of cancer cells to several cytotoxic nucleoside analogs. Thus, it may be used as a suicide gene in combined gene/chemotherapy treatment of cancer. To further characterize this potential suicide gene, we constructed two retroviral vectors that enabled the expression of Dm-dNK in cancer cells. One vector harbored the wild‑type enzyme that localized to the nucleus. The other vector harbored a mitochondrial localized mutant enzyme that was constructed by deleting the nuclear localization signal and fusing it to a mitochondrial import signal of cytochrome c oxidase. A thymidine kinase-deficient osteosarcoma cell line was transduced with the recombinant viruses. The sensitivity and bystander cell killing in the presence of pyrimidine nucleoside analogs (E)-5-(2-bromovinyl)‑2'‑deoxyuridine and 1-β-D-arabinofuranosylthymine were investigated. Tanshinone IIA is a constituent of Danshen; a traditional Chinese medicine used in the treatment of cardiovascular diseases. This study also looked at the influence of Tanshinone IIA on the bystander effect and the underlying mechanisms. We showed that sensitivity of the osteosarcoma cell line to the nucleoside analogs and the efficiency of bystander cell killing were independent of the subcellular localization of Dm-dNK. The enhanced effect of tanshinone IIA on the bystander effect was related to the increased expression of Cx43 and Cx26.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Zhao L, Dong X, He A, Zheng C, Johansson M, Karlsson A and Zheng X: Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria. Oncol Rep 34: 1487-1493, 2015.
APA
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M. ... Zheng, X. (2015). Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria. Oncology Reports, 34, 1487-1493. https://doi.org/10.3892/or.2015.4102
MLA
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M., Karlsson, A., Zheng, X."Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria". Oncology Reports 34.3 (2015): 1487-1493.
Chicago
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M., Karlsson, A., Zheng, X."Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria". Oncology Reports 34, no. 3 (2015): 1487-1493. https://doi.org/10.3892/or.2015.4102
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Zhao L, Dong X, He A, Zheng C, Johansson M, Karlsson A and Zheng X: Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria. Oncol Rep 34: 1487-1493, 2015.
APA
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M. ... Zheng, X. (2015). Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria. Oncology Reports, 34, 1487-1493. https://doi.org/10.3892/or.2015.4102
MLA
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M., Karlsson, A., Zheng, X."Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria". Oncology Reports 34.3 (2015): 1487-1493.
Chicago
Jiang, H., Zhao, L., Dong, X., He, A., Zheng, C., Johansson, M., Karlsson, A., Zheng, X."Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria". Oncology Reports 34, no. 3 (2015): 1487-1493. https://doi.org/10.3892/or.2015.4102
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