A secretomic study on human hepatocellular carcinoma multiple drug-resistant cell lines

  • Authors:
    • Yi Xiang
    • Yi Liu
    • Yixuan Yang
    • Huaidong Hu
    • Peng Hu
    • Hong Ren
    • Dazhi Zhang
  • View Affiliations

  • Published online on: July 3, 2015     https://doi.org/10.3892/or.2015.4106
  • Pages: 1249-1260
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Abstract

The aim of this study was to identify pivotal dysregulated proteins that are biomarkers for multiple drug resistance (MDR) of human hepatocellular carcinoma (HCC). The secretome profiles of the human HCC MDR cell line BEL7402/5-FU and its parental cell line BEL7402 were comparatively analyzed using isobaric tags for the relative and absolute quantification (iTRAQ)-coupled 2D LC-MS/MS. In total, 279 differentially expressed proteins were identified, of which, with a consistent result in the duplex test, 131 proteins were overexpressed in BEL7402/5-FU compared to its parental cell line, and 56 proteins were underexpressed. Several differentially expressed proteins determined by western blot analysis were also validated. The association of MDR with one of the highly regulated proteins, α-2-HS-glycoprotein (AHSG) was determined. This study detailed the application of iTRAQ technology to MDR biomarkers in the HCC cell secretome. The results showed that differentially expressed proteins that may be associated with MDR of HCC provide valuable additional information with regard to understanding the role of MDR.
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September-2015
Volume 34 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xiang Y, Liu Y, Yang Y, Hu H, Hu P, Ren H and Zhang D: A secretomic study on human hepatocellular carcinoma multiple drug-resistant cell lines. Oncol Rep 34: 1249-1260, 2015
APA
Xiang, Y., Liu, Y., Yang, Y., Hu, H., Hu, P., Ren, H., & Zhang, D. (2015). A secretomic study on human hepatocellular carcinoma multiple drug-resistant cell lines. Oncology Reports, 34, 1249-1260. https://doi.org/10.3892/or.2015.4106
MLA
Xiang, Y., Liu, Y., Yang, Y., Hu, H., Hu, P., Ren, H., Zhang, D."A secretomic study on human hepatocellular carcinoma multiple drug-resistant cell lines". Oncology Reports 34.3 (2015): 1249-1260.
Chicago
Xiang, Y., Liu, Y., Yang, Y., Hu, H., Hu, P., Ren, H., Zhang, D."A secretomic study on human hepatocellular carcinoma multiple drug-resistant cell lines". Oncology Reports 34, no. 3 (2015): 1249-1260. https://doi.org/10.3892/or.2015.4106