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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion

  • Authors:
    • Hongjun Wang
    • Yongzhi Wang
    • Zhaoshi Bao
    • Chuanbao Zhang
    • Yanwei Liu
    • Jinquan Cai
    • Chuanlu Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, P.R. China
  • Pages: 1503-1509
    |
    Published online on: July 13, 2015
       https://doi.org/10.3892/or.2015.4125
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Abstract

In the present study, in order to analyze abnormalities in DNA methylation in glioma, we utilized a large cohort methylation microarray (119 glioma samples). Genes associated with tumor grade progression were screened through Significance Analysis of Microarrays (SAM) in the methylation microarray. We found that Rab27b was hypomethylated in high-grade glioma (anaplastic gliomas and glioblastomas) compared with low-grade glioma (astrocytoma, oligodendrocytoma and oligoastrocytoma) (p=0.02). In 52 glioma samples, we determined both the methylation status of the Rab27b promoter region and protein expression, and confirmed a negative correlation between the methylation status and expression (p<0.01). Immunohistochemistry of 91 gliomas revealed that the Rab27b expression scores of high-grade glioma were higher than scores of low-grade gliomas (p<0.01). In high‑grade gliomas, patients haboring Rab27b hypomethylation or overexpression had unfavorable survival prognosis. Transwell invasion assays identified that invasive cell number of glioma U87 and LN229 cells decreased when Rab27b was knocked down. Decreased invasion partly resulted from reduced expression and activation of MMP-9 after Rab27b knockdown. Downregulation of Rab27b also suppressed tumor growth in vivo. Hypomethylated Rab27b was identified as a progression-associated and prognostic molecular marker of glioma.
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1 

Zhang W, Yan W, You G, Bao Z, Wang Y, Liu Y, You Y and Jiang T: Genome-wide DNA methylation profiling identifies ALDH1A3 promoter methylation as a prognostic predictor in G-CIMP- primary glioblastoma. Cancer Lett. 328:120–125. 2013. View Article : Google Scholar

2 

Yan W, Zhang W, You G, Zhang J, Han L, Bao Z, Wang Y, Liu Y, Jiang C, Kang C, et al: Molecular classification of gliomas based on whole genome gene expression: A systematic report of 225 samples from the Chinese Glioma Cooperative Group. Neuro Oncol. 14:1432–1440. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Wang Y, Li S, Chen L, You G, Bao Z, Yan W, Shi Z, Chen Y, Yao K, Zhang W, et al: Glioblastoma with an oligodendroglioma component: Distinct clinical behavior, genetic alterations, and outcome. Neuro Oncol. 14:518–525. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Wang H, Wang Y and Jiang C: Stromal protein periostin identified as a progression associated and prognostic biomarker in glioma via inducing an invasive and proliferative phenotype. Int J Oncol. 42:1716–1724. 2013.PubMed/NCBI

5 

Jones PA and Baylin SB: The epigenomics of cancer. Cell. 128:683–692. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Gaspar-Maia A, Alajem A, Meshorer E and Ramalho-Santos M: Open chromatin in pluripotency and reprogramming. Nat Rev Mol Cell Biol. 12:36–47. 2011. View Article : Google Scholar

7 

Feinberg AP and Tycko B: The history of cancer epigenetics. Nat Rev Cancer. 4:143–153. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Wang H, Feng Y, Bao Z, Jiang C, Yan W, Wang Y, Zhang C, Liu Y, Zhang Q, Zhang W, et al: Epigenetic silencing of KAZALD1 confers a better prognosis and is associated with malignant transformation/progression in glioma. Oncol Rep. 30:2089–2096. 2013.PubMed/NCBI

9 

Baylin SB: DNA methylation and gene silencing in cancer. Nat Clin Pract Oncol. 2(Suppl 1): S4–S11. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Ehrlich M: DNA methylation in cancer: Too much, but also too little. Oncogene. 21:5400–5413. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Cadieux B, Ching TT, van den Berg SR and Costello JF: Genome-wide hypomethylation in human glioblastomas associated with specific copy number alteration, methylenetetrahydrofolate reductase allele status, and increased proliferation. Cancer Res. 66:8469–8476. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Hendrix A, Maynard D, Pauwels P, Braems G, Denys H, van den Broecke R, Lambert J, van Belle S, Cocquyt V, Gespach C, et al: Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis. J Natl Cancer Inst. 102:866–880. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Li S, Yan C, Huang L, Qiu X, Wang Z and Jiang T: Molecular prognostic factors of anaplastic oligodendroglial tumors and its relationship: A single institutional review of 77 patients from China. Neuro Oncol. 14:109–116. 2012. View Article : Google Scholar :

14 

Hill VK, Ricketts C, Bieche I, vacher S, Gentle D, Lewis C, Maher ER and Latif F: Genome-wide DNA methylation profiling of CpG islands in breast cancer identifies novel genes associated with tumorigenicity. Cancer Res. 71:2988–2999. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Zhang W, Qiu XG, Chen BS, Li SW, Cui Y, Ren H and Jiang T: Antiangiogenic therapy with bevacizumab in recurrent malignant gliomas: Analysis of the response and core pathway aberrations. Chin Med J (Engl). 122:1250–1254. 2009.

16 

Wang F, Yang C, Song Y, Jiang Y and Ding Z: Periostin gene polymorphisms, protein levels and risk of incident coronary artery disease. Mol Biol Rep. 39:359–367. 2012. View Article : Google Scholar

17 

Iacob G and Dinca EB: Current data and strategy in glioblastoma multiforme. J Med Life. 2:386–393. 2009.

18 

Laffaire J, Everhard S, Idbaih A, Crinière E, Marie Y, de Reyniès A, Schiappa R, Mokhtari K, Hoang-Xuan K, Sanson M, et al: Methylation profiling identifies 2 groups of gliomas according to their tumorigenesis. Neuro Oncol. 13:84–98. 2011. View Article : Google Scholar :

19 

Lebrand C, Corti M, Goodson H, Cosson P, Cavalli V, Mayran N, Fauré J and Gruenberg J: Late endosome motility depends on lipids via the small GTPase Rab7. EMBO J. 21:1289–1300. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Tomshine JC, Severson SR, Wigle DA, Sun Z, Beleford DA, Shridhar V and Horazdovsky BF: Cell proliferation and epidermal growth factor signaling in non-small cell lung adenocarcinoma cell lines are dependent on Rin1. J Biol Chem. 284:26331–26339. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Wang Y, Bao Z, Zhang C, Liu Y, Cai J and Jiang C: Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion. Oncol Rep 34: 1503-1509, 2015.
APA
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., & Jiang, C. (2015). Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion. Oncology Reports, 34, 1503-1509. https://doi.org/10.3892/or.2015.4125
MLA
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., Jiang, C."Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion". Oncology Reports 34.3 (2015): 1503-1509.
Chicago
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., Jiang, C."Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion". Oncology Reports 34, no. 3 (2015): 1503-1509. https://doi.org/10.3892/or.2015.4125
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Wang Y, Bao Z, Zhang C, Liu Y, Cai J and Jiang C: Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion. Oncol Rep 34: 1503-1509, 2015.
APA
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., & Jiang, C. (2015). Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion. Oncology Reports, 34, 1503-1509. https://doi.org/10.3892/or.2015.4125
MLA
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., Jiang, C."Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion". Oncology Reports 34.3 (2015): 1503-1509.
Chicago
Wang, H., Wang, Y., Bao, Z., Zhang, C., Liu, Y., Cai, J., Jiang, C."Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion". Oncology Reports 34, no. 3 (2015): 1503-1509. https://doi.org/10.3892/or.2015.4125
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