Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
October-2015 Volume 34 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2015 Volume 34 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes

  • Authors:
    • Xingli Song
    • Yi Ren
    • Jing Zhang
    • Gang Wang
    • Xuedong Han
    • Wei Zheng
    • Linlin Zhen
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China, Department of Breast and Thyroid Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, P.R. China, Department of Breast Surgery, Xuzhou Tumor Hospital, Xuzhou, Jiangsu, P.R. China, Department of General Surgery, Beijing Aerospace General Hospital, Beijing, P.R. China
  • Pages: 1953-1960
    |
    Published online on: July 17, 2015
       https://doi.org/10.3892/or.2015.4136
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Doxorubicin is used to treat numerous types of tumors including breast cancer, yet dose-associated toxicities limit its clinical application. Here, we demonstrated a novel strategy by which to deliver doxorubicin to breast cancer cells by conjugating cancer cell-specific single-strand DNA aptamers with doxorubicin-encapsulated DOTAP:DOPE nanoparticles (NPs). We utilizing a whole-cell-SELEX strategy, and 4T1 cells with high invasive and metastatic potential were used as target cells, while non-invasive and non-metastatic 67NR cells were used as subtractive cells. Ten potential aptamers were generated after multi-pool selection. Studies on the selected aptamers revealed that SRZ1 had the highest and specific binding affinity to 4T1 cells. Then we developed SRZ1 aptamer-carried DOTAP:DOPE-DOX NPs. In vitro uptake results which were conducted by FACS indicated that the aptamer significantly promoted the uptake efficiency of DOTAP:DOPE-DOX NPs by 4T1 cells. ATPlite assay was performed to test 4T1, 67NR and NMuMG cell viability after treatment with free doxorubicin, DOTAP:DOPE-DOX particles and aptamer‑loaded DOTAP:DOPE-DOX particles. As expected, the aptamers effectively enhanced accumulation of doxorubicin in the 4T1 tumor tissues as determined by in vivo mouse body images and biodistribution analysis. Consistent with the in vitro findings, aptamer-conjugated doxorubicin-loaded DOTAP:DOPE particles markedly suppressed tumor growth and significantly increased the survival rate of 4T1 tumor-bearing mice. These studies demonstrated that aptamer SRZ1 could be a promising molecule for chemotherapeutic drug targeting deliver.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Wang B, He M and Wang L, Engelgau MM, Zhao W and Wang L: Breast cancer screening among adult women in China, 2010. Prev Chronic Dis. 10:E1832013. View Article : Google Scholar : PubMed/NCBI

2 

MacDonald V: Chemotherapy: managing side effects and safe handling. Can Vet J. 50:665–668. 2009.PubMed/NCBI

3 

Lin X, Gao R, Zhang Y, Qi N, Zhang Y, Zhang K, He H and Tang X: Lipid nanoparticles for chemotherapeutic applications: strategies to improve anticancer efficacy. Expert Opin Drug Deliv. 9:767–781. 2012. View Article : Google Scholar : PubMed/NCBI

4 

De Jong WH and Borm PJ: Drug delivery and nanoparticles: applications and hazards. Int J Nanomed. 3:133–149. 2008. View Article : Google Scholar

5 

Shohdy KS and Alfaar AS: Nanoparticles targeting mechanisms in cancer therapy: current limitations and emerging solutions. Ther Deliv. 4:1197–1209. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Bastakoti BP, Torad NL and Yamauchi Y: Polymeric micelle assembly for the direct synthesis of platinum-decorated mesoporous TiO2 toward highly selective sensing of acetaldehyde. ACS Appl Mater Interfaces. 6:854–860. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Quan L, Zhang Y, Crielaard BJ, Dusad A, Lele SM, Rijcken CJ, Metselaar JM, Kostková H, Etrych T, Ulbrich K, et al: Nano-medicines for inflammatory arthritis: head-to-head comparison of glucocorticoid-containing polymers, micelles, and liposomes. ACS Nano. 8:458–466. 2014. View Article : Google Scholar :

8 

Chang DK, Li PC, Lu RM, Jane WN and Wu HC: Peptide-mediated liposomal doxorubicin enhances drug delivery efficiency and therapeutic efficacy in animal models. PLoS One. 8:e832392013. View Article : Google Scholar

9 

Wang Q, Zhuang X, Mu J, Deng ZB, Jiang H, Zhang L, Xiang X, Wang B, Yan J, Miller D, et al: Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids. Nat Commun. 4:18672013. View Article : Google Scholar : PubMed/NCBI

10 

Park K: Lessons learned from thermosensitive liposomes for improved chemotherapy. J Control Release. 174:2192014. View Article : Google Scholar : PubMed/NCBI

11 

Sabnis N and Lacko AG: Drug delivery via lipoprotein-based carriers: answering the challenges in systemic therapeutics. Ther Deliv. 3:599–608. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Lacko AG, Nair M, Prokai L and McConathy WJ: Prospects and challenges of the development of lipoprotein-based formulations for anti-cancer drugs. Expert Opin Drug Deliv. 4:665–675. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Lassalle HP, Marchal S, Guillemin F, Reinhard A and Bezdetnaya L: Aptamers as remarkable diagnostic and therapeutic agents in cancer treatment. Curr Drug Metab. 13:1130–1144. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Bunka DH, Platonova O and Stockley PG: Development of aptamer therapeutics. Curr Opin Pharmacol. 10:557–562. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Shi H, Cui W, He X, Guo Q, Wang K, Ye X and Tang J: Whole cell-SELEX aptamers for highly specific fluorescence molecular imaging of carcinomas in vivo. PLoS One. 8:e704762013. View Article : Google Scholar : PubMed/NCBI

16 

Meyer S, Maufort JP, Nie J, Stewart R, McIntosh BE, Conti LR, Ahmad KM, Soh HT and Thomson JA: Development of an efficient targeted cell-SELEX procedure for DNA aptamer reagents. PLoS One. 8:e717982013. View Article : Google Scholar : PubMed/NCBI

17 

Dassie JP and Giangrande PH: Current progress on aptamer-targeted oligonucleotide therapeutics. Ther Deliv. 4:1527–1546. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Subramanian N, Raghunathan V, Kanwar JR, Kanwar RK, Elchuri SV, Khetan V and Krishnakumar S: Target-specific delivery of doxorubicin to retinoblastoma using epithelial cell adhesion molecule aptamer. Mol Vis. 18:2783–2795. 2012.PubMed/NCBI

19 

Tacar O, Sriamornsak P and Dass CR: Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol. 65:157–170. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Carvalho C, Santos RX, Cardoso S, Correia S, Oliveira PJ, Santos MS and Moreira PI: Doxorubicin: the good, the bad and the ugly effect. Curr Med Chem. 16:3267–3285. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Speelmans G, Staffhorst RW, De Kruijff B and De Wolf FA: Transport studies of doxorubicin in model membranes indicate a difference in passive diffusion across and binding at the outer and inner leaflets of the plasma membrane. Biochemistry. 33:13761–13768. 1994. View Article : Google Scholar : PubMed/NCBI

22 

Kamba SA, Ismail M, Hussein-Al-Ali SH, Ibrahim TA and Zakaria ZA: In vitro delivery and controlled release of doxorubicin for targeting osteosarcoma bone cancer. Molecules. 18:10580–10598. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Shaikh IM, Tan KB, Chaudhury A, Liu Y, Tan BJ, Tan BM and Chiu GN: Liposome co-encapsulation of synergistic combination of irinotecan and doxorubicin for the treatment of intraperitoneally grown ovarian tumor xenograft. J Control Release. 172:852–861. 2013. View Article : Google Scholar

24 

Zhaorigetu S, Rodriguez-Aguayo C, Sood AK, Lopez-Berestein G and Walton BL: Delivery of negatively charged liposomes into the atherosclerotic plaque of apolipoprotein E-deficient mouse aortic tissue. J Liposome Res. 24:182–190. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Alavi SE, Esfahani MK, Alavi F, Movahedi F and Akbarzadeh A: Drug delivery of hydroxyurea to breast cancer using liposomes. Indian J Clin Biochem. 28:299–302. 2013. View Article : Google Scholar :

26 

Remaut K, Lucas B, Braeckmans K, Sanders NN, Demeester J and De Smedt SC: Delivery of phosphodiester oligonucleotides: can DOTAP/DOPE liposomes do the trick? Biochemistry. 45:1755–1764. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Ciani L, Casini A, Gabbiani C, Ristori S, Messori L and Martini G: DOTAP/DOPE and DC- Chol/DOPE lipoplexes for gene delivery studied by circular dichroism and other biophysical techniques. Biophys Chem. 127:213–220. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Song X, Ren Y, Zhang J, Wang G, Han X, Zheng W and Zhen L: Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes. Oncol Rep 34: 1953-1960, 2015.
APA
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., & Zhen, L. (2015). Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes. Oncology Reports, 34, 1953-1960. https://doi.org/10.3892/or.2015.4136
MLA
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., Zhen, L."Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes". Oncology Reports 34.4 (2015): 1953-1960.
Chicago
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., Zhen, L."Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes". Oncology Reports 34, no. 4 (2015): 1953-1960. https://doi.org/10.3892/or.2015.4136
Copy and paste a formatted citation
x
Spandidos Publications style
Song X, Ren Y, Zhang J, Wang G, Han X, Zheng W and Zhen L: Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes. Oncol Rep 34: 1953-1960, 2015.
APA
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., & Zhen, L. (2015). Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes. Oncology Reports, 34, 1953-1960. https://doi.org/10.3892/or.2015.4136
MLA
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., Zhen, L."Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes". Oncology Reports 34.4 (2015): 1953-1960.
Chicago
Song, X., Ren, Y., Zhang, J., Wang, G., Han, X., Zheng, W., Zhen, L."Targeted delivery of doxorubicin to breast cancer cells by aptamer functionalized DOTAP/DOPE liposomes". Oncology Reports 34, no. 4 (2015): 1953-1960. https://doi.org/10.3892/or.2015.4136
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team