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Article

Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway

Retraction in: /10.3892/or.2025.8960
  • Authors:
    • Qiong Fan
    • Mei-Ting Qiu
    • Zhu Zhu
    • Jin-Hua Zhou
    • Limo Chen
    • Ye Zhou
    • Wei Gu
    • Li‑Hua Wang
    • Zhu-Nan Li
    • Ying Xu
    • Wei-Wei Cheng
    • Dan Wu
    • Wei Bao
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200030, P.R. China, Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
  • Pages: 1787-1794
    |
    Published online on: July 22, 2015
       https://doi.org/10.3892/or.2015.4143
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Abstract

Epithelial-mesenchymal transition (EMT) is associated with the metastasis and poor prognosis of cervical cancer. However, the underlying mechanisms are poorly defined. In the present study, we investigated whether Twist plays a direct role in human cervical cancer using immunohistochemical and western blot analyses. Immunohistochemical analysis revealed that Twist is highly expressed in cervical cancer, which correlates with poor tumor pathological differentiation or lymph node metastasis (P<0.05). Depletion of Twist by stable shRNA-mediated knockdown decreased the migratory ability of cancer cell lines in vitro. Suppression or overexpression of Twist also resulted in an altered expression of the molecular mediators of EMT. Furthermore, exogenous TGF-β promoted EMT by upregulating the expression of Twist through the TGF-β/Smad3 pathway, and this effect was eliminated by Twist depletion in cancer cells as demonstrated in the in vitro study. The use of in vivo models revealed a decreased tumor proliferation potential in Twist-depleted cancer cells. The results suggested a novel function for Twist in the promotion of EMT via TGF-β/Smad3 signaling pathway. Thus, Twist constitutes a potential therapeutic target in human cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fan Q, Qiu M, Zhu Z, Zhou J, Chen L, Zhou Y, Gu W, Wang LH, Li Z, Xu Y, Xu Y, et al: Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960. Oncol Rep 34: 1787-1794, 2015.
APA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y. ... Bao, W. (2015). Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960. Oncology Reports, 34, 1787-1794. https://doi.org/10.3892/or.2015.4143
MLA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960". Oncology Reports 34.4 (2015): 1787-1794.
Chicago
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960". Oncology Reports 34, no. 4 (2015): 1787-1794. https://doi.org/10.3892/or.2015.4143
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Q, Qiu M, Zhu Z, Zhou J, Chen L, Zhou Y, Gu W, Wang LH, Li Z, Xu Y, Xu Y, et al: Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960. Oncol Rep 34: 1787-1794, 2015.
APA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y. ... Bao, W. (2015). Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960. Oncology Reports, 34, 1787-1794. https://doi.org/10.3892/or.2015.4143
MLA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960". Oncology Reports 34.4 (2015): 1787-1794.
Chicago
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway Retraction in /10.3892/or.2025.8960". Oncology Reports 34, no. 4 (2015): 1787-1794. https://doi.org/10.3892/or.2015.4143
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