Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway

  • Authors:
    • Qiong Fan
    • Mei-Ting Qiu
    • Zhu Zhu
    • Jin-Hua Zhou
    • Limo Chen
    • Ye Zhou
    • Wei Gu
    • Li‑Hua Wang
    • Zhu-Nan Li
    • Ying Xu
    • Wei-Wei Cheng
    • Dan Wu
    • Wei Bao
  • View Affiliations

  • Published online on: July 22, 2015     https://doi.org/10.3892/or.2015.4143
  • Pages: 1787-1794
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Abstract

Epithelial-mesenchymal transition (EMT) is associated with the metastasis and poor prognosis of cervical cancer. However, the underlying mechanisms are poorly defined. In the present study, we investigated whether Twist plays a direct role in human cervical cancer using immunohistochemical and western blot analyses. Immunohistochemical analysis revealed that Twist is highly expressed in cervical cancer, which correlates with poor tumor pathological differentiation or lymph node metastasis (P<0.05). Depletion of Twist by stable shRNA-mediated knockdown decreased the migratory ability of cancer cell lines in vitro. Suppression or overexpression of Twist also resulted in an altered expression of the molecular mediators of EMT. Furthermore, exogenous TGF-β promoted EMT by upregulating the expression of Twist through the TGF-β/Smad3 pathway, and this effect was eliminated by Twist depletion in cancer cells as demonstrated in the in vitro study. The use of in vivo models revealed a decreased tumor proliferation potential in Twist-depleted cancer cells. The results suggested a novel function for Twist in the promotion of EMT via TGF-β/Smad3 signaling pathway. Thus, Twist constitutes a potential therapeutic target in human cervical cancer.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fan Q, Qiu M, Zhu Z, Zhou J, Chen L, Zhou Y, Gu W, Wang LH, Li Z, Xu Y, Xu Y, et al: Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway. Oncol Rep 34: 1787-1794, 2015.
APA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y. ... Bao, W. (2015). Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway. Oncology Reports, 34, 1787-1794. https://doi.org/10.3892/or.2015.4143
MLA
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway". Oncology Reports 34.4 (2015): 1787-1794.
Chicago
Fan, Q., Qiu, M., Zhu, Z., Zhou, J., Chen, L., Zhou, Y., Gu, W., Wang, L., Li, Z., Xu, Y., Cheng, W., Wu, D., Bao, W."Twist induces epithelial-mesenchymal transition in cervical carcinogenesis by regulating the TGF-β/Smad3 signaling pathway". Oncology Reports 34, no. 4 (2015): 1787-1794. https://doi.org/10.3892/or.2015.4143