Effect of Raf kinase inhibitor protein expression on malignant biological behavior and progression of colorectal cancer

  • Authors:
    • Yun Wang
    • Ling-Yun Wang
    • Fen Feng
    • Yang Zhao
    • Ma-Yan Huang
    • Qiong Shao
    • Cui Chen
    • Hui Sheng
    • Dong-Liang Chen
    • Zhao-Lei Zeng
    • Rui-Hua Xu
    • Yu-Hong Li
  • View Affiliations

  • Published online on: July 28, 2015     https://doi.org/10.3892/or.2015.4157
  • Pages: 2106-2114
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The Raf kinase inhibitor protein (RKIP) is a novel metastasis suppressor. RKIP was previously found to have low expression in a colorectal cancer (CRC) patient cohort by immunohistochemistry. However, the role of RKIP in CRC remains undetermined. In the present study, immunohistochemistry was performed to compare RKIP expression between 129 paired stage II CRC and adjacent non-tumorous tissues. The correlations between clinical parameters, prognosis and RKIP expression were evaluated. To investigate the effect of RKIP on proliferation and metastasis, RKIP was overexpressed and knocked down in colon cancer cell lines. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Transwell and wound-healing assays were performed. Murine models were established to confirm the influence of RKIP on malignant tumor phenotypes in vivo. Our results showed that RKIP expression was significantly decreased in the CRC tissues compared to the adjacent non‑cancerous tissues (p<0.001) and was correlated with the risk of relapse in stage II CRC (p<0.05). Overexpression of RKIP suppressed HCT116 cell metastasis in vitro and in vivo, whereas knockdown of RKIP expression in SW480 cells and its murine model increased metastatic ability (p<0.05). No effect of RKIP on cell proliferation in CRC was observed. These data suggest that RKIP is an important metastasis-suppressor gene in CRC. The re-expression of RKIP could be a potential therapeutic target for antimetastatic strategies for CRC.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang L, Feng F, Zhao Y, Huang M, Shao Q, Chen C, Sheng H, Chen D, Zeng Z, Zeng Z, et al: Effect of Raf kinase inhibitor protein expression on malignant biological behavior and progression of colorectal cancer. Oncol Rep 34: 2106-2114, 2015
APA
Wang, Y., Wang, L., Feng, F., Zhao, Y., Huang, M., Shao, Q. ... Li, Y. (2015). Effect of Raf kinase inhibitor protein expression on malignant biological behavior and progression of colorectal cancer. Oncology Reports, 34, 2106-2114. https://doi.org/10.3892/or.2015.4157
MLA
Wang, Y., Wang, L., Feng, F., Zhao, Y., Huang, M., Shao, Q., Chen, C., Sheng, H., Chen, D., Zeng, Z., Xu, R., Li, Y."Effect of Raf kinase inhibitor protein expression on malignant biological behavior and progression of colorectal cancer". Oncology Reports 34.4 (2015): 2106-2114.
Chicago
Wang, Y., Wang, L., Feng, F., Zhao, Y., Huang, M., Shao, Q., Chen, C., Sheng, H., Chen, D., Zeng, Z., Xu, R., Li, Y."Effect of Raf kinase inhibitor protein expression on malignant biological behavior and progression of colorectal cancer". Oncology Reports 34, no. 4 (2015): 2106-2114. https://doi.org/10.3892/or.2015.4157